Publications related to Focus stacking

Laser engraving device for multiple laser beams

The present invention concerns a laser engraving device (1) for engraving a target surface (17). The laser engraving device (1) comprises: a beam splitter (7) for dividing a processing laser beam (25) into a plurality of engraving laser beams (27); a first ...

2020

Assessing microlens quality based on 3D irradiance measurement at the focal spot area

Toralf Scharf, Wilfried Noell, Jeremy Béguelin, Michail Symeonidis

During the fabrication process of microlenses, characterization is essential for two purposes: evaluate the optical quality of the element and provide surface information feedback for process optimization. However, no technique can fulfill these two object ...

2018

A Point-Spread-Function-Aware Filtered Backprojection Algorithm for Focal-Plane-Scanning Optical Projection Tomography

Michael Stefan Daniel Liebling

Traditional optical projection tomography requires the use of low numerical aperture objectives in order to achieve a large depth of field and approximate parallel projection geometry. However, low NA objectives suffer from poor resolution, resulting in bl ...

2016

Functional and Structural Imaging with Optical Coherence Microscopy : Principles & Applications

Arno Pino Bouwens

Fluorescence microscopy techniques are well established research tools and have proven their use in a large variety of biomedical applications. Microscopic molecular contrast is achieved by imaging fluorescent dyes that bind specifically to a molecule of i ...

EPFL2013

Focused light delivery and all optical scanning from a multimode optical fiber using digital phase conjugation

Demetri Psaltis, Christophe Moser, Salma Farahi

We demonstrate a digital phase conjugation technique for generating a sharp focus point at the end of a multimode optical fiber. A sharp focus is experimentally obtained at the distal end of a 200 mu m core multimode fiber. By recording the digital hologra ...

Spie-Int Soc Optical Engineering2013

Extended depth-of-focus by digital holographic microscopy

Christian Depeursinge, Tristan Colomb, Etienne Cuche, Jonas Kühn, Nicolas Pavillon

A recurrent problem in microscopy is the finite depth-of-focus linked to the NA of microscope objectives. Digital holographic microscopy (DHM) has the unique feature of being able to numerically change the focus from a single hologram without the need of m ...

Optical Society of America2010

Extended Focus Optical Coherence Microscopy : Theory and Application

Martin Villiger

Optical coherence tomography (OCT) is an interferometric imaging technique that can provide depth resolved cross-sectional views of biological tissue. OCT employs light with low temporal and yet high spatial coherence. The sample is illuminated point by po ...

EPFL2010

High Resolution Interference Microscopy: 3D measurement of focused light at 405nm applied to optical disc

Hans Peter Herzig, Toralf Scharf, Myun Sik Kim

High capacity data storage on DVD and optical discs is based on focused light beams. The characterization of light fields within the focus point is a very difficult task, which requires a high-resolution method like scanning near field optical microscopy ( ...

2009

Steerable filters for orientation estimation and localization of fluorescent dipoles

Michaël Unser, Theo Lasser, Iwan Märki, Stefan Geissbühler, François Aguet

Fluorescence localization microscopy (i.e., PALM, STORM) has enabled optical imaging at nanometer-scale resolutions. The localization algorithms used in these techniques rely on fitting a 2-D Gaussian to the in-focus image of individual fluorophores. For f ...

Ieee Service Center, 445 Hoes Lane, Po Box 1331, Piscataway, Nj 08855-1331 Usa2009

On the complex three-dimensional amplitude point spread function of lenses and microscope objectives: theoretical aspects, simulations and measurements by digital holography

Christian Depeursinge, Tristan Colomb, Frédéric Montfort, Pierre Marquet, Florian Charrière, Jonas Kühn, Mihaela Marian

The point spread function is widely used to characterize the three-dimensional imaging capabilities of an optical system. Usually, attention is paid only to the intensity point spread function, whereas the phase point spread function is most often neglecte ...

2007

Single molecule detection on surfaces

Marcel Leutenegger

Monitoring biological relevant reactions on the single molecule level based on fluorescence spectroscopy techniques has become one of the most promising approaches for understanding a variety of phenomena in biophysics, biochemistry and life science. By ap ...

EPFL2007

Fast focus field calculations

Theo Lasser, Marcel Leutenegger, Ramachandra Rao, Rainer Leitgeb

We present a fast calculation of the electromagnetic field near the focus of an objective with a high numerical aperture (NA). Instead of direct integration, the vectorial Debye diffraction integral is evaluated with the fast Fourier transform for calculat ...

2006

Fluorescence correlation spectroscopy : simulations and bio-chemical applications based on solid immersion lens concept

Ramachandra Rao

Fluorescence Correlation Spectroscopy (FCS) has become an important tool for measuring diffusion, concentration and molecular interactions in biomolecular systems. Two major requirements for FCS are good collection efficiency and a small and well defined e ...

EPFL2006