Publications related to Microtome

Controlling crystal cleavage in focused ion beam shaped specimens for surface spectroscopy

Philip Johannes Walter Moll, Matthias Carsten Putzke, Andrew Scott Hunter

Our understanding of quantum materials is commonly based on precise determinations of their electronic spectrum by spectroscopic means, most notably angle-resolved photoemission spectroscopy (ARPES) and scanning tunneling microscopy. Both require atomicall ...

Aip Publishing2024

Deep Learning Enables Individual Xenograft Cell Classification in Histological Images by Analysis of Contextual Features

Michaël Unser, Cathrin Brisken, Daniel Sage, Olivier Burri, Quentin Juppet, Fabio De Martino, Martin Weigert

Patient-Derived Xenografts (PDXs) are the preclinical models which best recapitulate inter- and intra-patient complexity of human breast malignancies, and are also emerging as useful tools to study the normal breast epithelium. However, data analysis gener ...

2021

Adaptation and Optimization of an Intraneural Electrode to Interface with the Cervical Vagus Nerve

Silvestro Micera, Ivo Strauss

The modulation of the cervical vagus nerve (VN) using neural electrodes has shown great potential to treat cardiovascular, inflammatory, intestinal, or respiratory dysfunctions. Intraneural electrodes have shown great potentials for other neuroprosthetic a ...

IEEE2021

Synchrotron-based phase-contrast micro-CT as a tool for understanding pulmonary vascular pathobiology and the 3-D microanatomy of alveolar capillary dysplasia

Goran Lovric

This study aimed to explore the value of synchrotron-based phase-contrast microcomputed tomography (micro-CT) in pulmonary vascular pathobiology. The microanatomy of the lung is complex with intricate branching patterns. Tissue sections are therefore diffi ...

AMER PHYSIOLOGICAL SOC2020

High-throughput and adaptive super-resolution fluorescence microscopy of organelles

Dora Mahecic

Fluorescence super-resolution microscopy has allowed unprecedented insight into the workings of biological systems below the diffraction limit of light. Over the past decade, it has overcome several challenges to deliver 3D, multi-color and faster imaging ...

EPFL2020

Cellular Uptake and Intracellular Trafficking of Poly(N-(2-Hydroxypropyl) Methacrylamide)

Anders Meibom, Harm-Anton Klok, Graham Knott, Stéphane Laurent Escrig, Olivier Burri, Arne Seitz, Claudia Battistella, Romain Guiet

Cellular uptake and intracellular trafficking of polymer conjugates or polymer nanoparticles is typically monitored using fluorescence-based techniques such as confocal microscopy. While these methods have provided a wealth of insight into the internalizat ...

American Chemical Society2019

Development of Sample-Adaptable Holders for Lightsheet Microscopy

Thierry Laroche, Olivier Burri, Arne Seitz, Lalit Kumar Dubey

Multi-user core microscopy facilities are often faced with the challenge to adapt or modify existing instruments. This is essential in order to fulfill the requirements of the user community, who wants to image a wide range of model organisms with varying ...

2019

Block Face Scanning Electron Microscopy of Fluorescently Labeled Axons Without Using Near Infra-Red Branding

Graham Knott, Catherine Maclachlan

In this article, we describe the method that allows fluorescently tagged structures such as axons to be targeted for electron microscopy (EM) analysis without the need to convert their labels into electron dense stains, introduce any fiducial marks, or ima ...

2018

Microfluidic network device

Martinus Gijs, Ata Tuna Ciftlik, Diego Gabriel Dupouy, Pierre Joris

Microfluidic network device (2) configured to supply reagents to a biological tissue sampling device (1), comprising a plurality of microfluidic inlet channels (12) connected to respective sources of said reagents, at least one common outlet channel (22), ...

2017

Nanotoxicology: 2D analysis in a 3D matrix

Anders Meibom, Louise Helene Soegaard Jensen

Engineered nanoparticles are the fastest growing nanotechnology product, and of increasing environmental concern. Examples include antimicrobial silver nanoparticles, iron oxide particles used in bulk scale for environmental remediation, and micro- and nan ...

2016

Effects of large scale bridging in load controlled fatigue delamination of unidirectional carbon-epoxy specimens

John Botsis, Joël Cugnoni, Ebrahim Farmand Ashtiani

Fatigue delamination growth in composites is accompanied by large scale bridging (LSB) that yields important toughening effects. However, the extent of this mechanism depends on the laminate geometry rendering its modeling a challenging task. This work pre ...

Elsevier2016

Investigation of resins suitable for the preparation of biological sample for 3-D electron microscopy

Giovanni Longo

In the last two decades, the third-dimension has become a focus of attention in electron microscopy to better understand the interactions within subcellular compartments. Initially, transmission electron tomography (TEM tomography) was introduced to image ...

Elsevier2015

NeuroMorph: A Toolset for the Morphometric Analysis and Visualization of 3D Models Derived from Electron Microscopy Image Stacks

Pascal Fua, Graham Knott, Corrado Cali, Biagio Nigro, Anne Alison Jorstad, Marta Wawrzyniak

Serial electron microscopy imaging is crucial for exploring the structure of cells and tissues. The development of block face scanning electron microscopy methods and their ability to capture large image stacks, some with near isotropic voxels, is proving ...

Humana Press2015

Nanoskiving Core-Shell Nanowires: A New Fabrication Method for Nano-optics

Anna Fontcuberta i Morral, Martin Heiss, Eleonora Russo, Yannik Fontana

This paper describes the fabrication of functional optical devices by sectioning quantum-dot-in-nanowires systems with predefined lengths and orientations. This fabrication process requires only two steps, embedding the nanowires in epoxy and using an ultr ...

Amer Chemical Soc2014

A diamond-based scanning probe spin sensor operating at low temperature in ultra-high vacuum

Klaus Kern, Markus Ternes

We present the design and performance of an ultra-high vacuum (UHV) low temperature scanning probe microscope employing the nitrogen-vacancy color center in diamond as an ultrasensitive magnetic field sensor. Using this center as an atomic-size scanning pr ...

Amer Inst Physics2014

Fabrication of Biocompatible Monolithic Microchannels with High Pressure-Resistance Using Direct Polymerization of PEG-Modified PMMA

Martinus Gijs, Ata Tuna Ciftlik, Elena Bianchi

Withstanding high pressures in polymeric microchannels is an important requirement for many biological applications. Here, a simple direct polymerization through a polyester photomask is applied to obtain monolithic polyethylene glycol (PEG)-modified poly( ...

Wiley-Blackwell2014

Sample preparation methods for femtosecond electron diffraction experiments

Helmuth Berger

Exploring the dynamics of Charge density wave system 1T-TaS2 via femtosecond electron diffraction demonstrated the power of this technique for studying ultrafast structural phenomena in strongly correlated electron materials [1]. The results revealed first ...

Elsevier Science Bv2013

Imaging green fluorescent protein-labeled neurons using light and electron microscopy

Graham Knott

The ability to observe axons and dendrites with transmission electron microscopy (EM) after they have been previously imaged live with laser-scanning microscopy is a useful technique to study their synaptic connectivity. This protocol provides a detailed m ...

2013

A combined experimental/numerical study of the scaling effects on mode I delamination of GFRP

Anastasios Vassilopoulos, John Botsis, Behzad Dehghan Manshadi

Bridging by intact fibers is an important toughening mechanism in composite materials. However, a direct experimental evaluation of its contribution is difficult to achieve and in the case of large scale bridging the thickness, h, may have an important inf ...

Elsevier2013

Universal sample holder

Graham Knott

This device is for holding samples during their preparation prior to imaging in the electron microscope. The design means it can be transferred between the light and electron microscopes as well as trimming devices used to prepare the final sample. It can ...

2013