Publications related to Capillary electrophoresis–mass spectrometry

New Approach for the Identification of Isobaric and Isomeric Metabolites

Thomas Rizzo, Ahmed Ben Faleh, Stephan Warnke, Ali H Abikhodr, Teun Van Wieringen

The structural elucidation of metabolite molecules is important in many branches of the life sciences. However, the isomeric and isobaric complexity of metabolites makes their identification extremely challenging, and analytical standards are often require ...

2023

Advancing Glycan Analysis: Cryogenic Ion Spectroscopy for Database-Driven Identification

Ali H Abikhodr

Glycans play a pivotal role in both physiological processes; however, the field of glycobiology remains relatively understudied within biochemistry. The intricate complexity of glycans, coupled with their abundance of isomeric forms, presents formidable ch ...

EPFL2023

Identification of Mobility-Resolved N-Glycan Isomers

Thomas Rizzo, Ahmed Ben Faleh, Stephan Warnke, Priyanka Bansal, Robert Paul Pellegrinelli

Glycan analysis has evolved considerably during the last decade. The advent of high-resolution ion-mobility spectrometry has enabled the separation of isomers with only the slightest of structural differences. However, the ability to separate such species ...

AMER CHEMICAL SOC2022

A gas phase approach for glycan analysis: Combining ultrahigh-resolution ion mobility spectrometry with cryogenic vibrational spectroscopy

Ahmed Ben Faleh

The last decades have been punctuated by the development of revolutionary analytical technologies that have enabled the analysis of biomolecular structures and unraveled the roles they play in biological systems. However, certain classes of biomolecules re ...

EPFL2021

Optimization of a Py-GC/MS method for silicone -based lubricants analysis

Christophe Roussel, Céline Burnier

Condom evidence can be analysed using several analytical techniques, such as FTIR, MALDI-MS or DART-TOF-MS, but the only one that was used on real samples for transfer and persistence studies in the context of sexual assault or rape cases was Py-GC/MS. How ...

2020

Imaging Catalysis: Operando Investigation of the CO2 Hydrogenation Reaction Dynamics by Means of Infrared Thermography

Andreas Züttel, Emad Oveisi, Emanuele Moioli, Loris Giovanni Lombardo, Kun Zhao, Robin Tobias Andreas Mutschler

Infrared thermography is a powerful tool to investigate the dynamic evolution of temperature in chemical reactions. The CO2 hydrogenation reaction is an ideal model reaction to assess the presented technique due to its high exothermicity. Various dynamic e ...

AMER CHEMICAL SOC2020

Mass Spectrometry Methods for Bacterial Infection Diagnosis and Cancer Analysis

Yingdi Zhu

Matrix-assisted laser desorption/ionisation (MALDI) time-of-flight (TOF) mass spectrometry is an important analytical tool in chemistry and biology. Bearing the advantages of easy-to-use, fast measurements and relatively simple data interpretation, MALDI-T ...

EPFL2019

Investigation of the heterogeneously catalysed gas phase CO2 hydrogenation reaction: Development of analysis methods and reaction analysis on pristine metal catalysts

Robin Tobias Andreas Mutschler

The aim of this thesis is to contribute to the development of analysis methods that enable an improved investigation of the CO2 hydrogenation reaction such as quantitative mass spectrometry and infrared thermography. Furthermore, the contribution is in the ...

EPFL2019

Combining Ion Mobility and Cryogenic Spectroscopy for Structural and Analytical Studies of Biomolecular Ions

Thomas Rizzo, Michael Zachary Kamrath

Ion mobility spectrometry (IMS) has become a valuable tool in biophysical and bioanalytical chemistry because of its ability to separate and characterize the structure of gas-phase biomolecular ions on the basis of their collisional cross section (CCS). It ...

2018

Separation of blood microsamples by exploiting sedimentation at the microscale

Philippe Renaud, David Forchelet

Microsample analysis is highly bene cial in blood-based testing where cutting-edge bioanalytical technologies enable the analysis of volumes down to a few tens of microliters. Despite the availability of analytical methods, the di culty in obtaining high-q ...

Nature Research2018

New ionization and tagging methods for mass spectrometry

Xiaoqin Zhong

Mass spectrometry (MS) is an essential detection tool in bioanalytical chemistry owing to its exceptional selectivity and sensitivity, paired with rapid analyte identification and quantification. In this thesis, two classical MS and a newly developed ioniz ...

EPFL2017

Synthesis and In Vitro (Anticancer) Evaluation of eta(6)-Arene Ruthenium Complexes Bearing Stannyl Ligands

Paul Joseph Dyson, Tina Riedel

Treatment of the known half-sandwich complexes of the type [(eta(6)-C6H6)RuCl2(P(OR)(3))] (R = Me or Ph) with SnCl2 yielded three new half-sandwich ruthenium complexes (C1-C3): [(eta(6)-C6H6)RuCl(SnCl3)(P(OMe)(3))] (C1), [(eta(6)-C6H6)RuCl(SnCl3)(P(OPh)(3) ...

2017

Mass Barcode Signal Amplification for Multiplex Allergy Diagnosis by MALDI-MS

Hubert Girault, Natalia Gasilova, Liang Qiao, Xiaoqin Zhong

A highly sensitive method based on mass-barcoded gold nanoparticles (AuNPs) and immunomagnetic separation has been developed for multiplex allergy diagnosis by MALDI mass spectrometry in a component-resolved manner. Different analytical probes were prepare ...

American Chemical Society2016

Middle-down approaches for mass spectrometry-based protein identification and characterization

Kristina Srzentic

Mass spectrometry (MS) has emerged over the last two decades as the analytical technique of choice in systems-level protein studies, known as proteomics. Two are the MS-based approaches generally applied to proteomics: bottom-up (BU), which relies on the p ...

EPFL2016

Extension of least squares spectral resolution algorithm to high-resolution lipidomics data

Adrian Schmid

Lipidomics, which focuses on the global study of molecular lipids in biological systems, has been driven tremendously by technical advances in mass spectrometry (MS) instrumentation, particularly high-resolution MS. This requires powerful computational too ...

Elsevier2016

A hyphenated SMPS-ICPMS coupling setup: Size-resolved element specific analysis of airborne nanoparticles

Christian Ludwig, Mohamed Tarik, Adrian Hess

Physical and chemical characterization of gas borne particles in the nanometer to sub micrometer size range includes knowledge about particle size distribution and chemical composition, possibly with a high time resolution. However, most of the available t ...

Elsevier Sci Ltd2015

Highly sensitive detection of five typical fluoroquinolones in low-fat milk by field-enhanced sample injection based CE in bubble cell capillary

Hubert Girault, Natalia Gasilova, Liang Qiao, Yan Deng

Fluoroquinolones are a group of synthetic antibiotics with a broad activity spectrum against mycoplasma, gram-positive and gram-negative bacteria. Due to the extensive use of fluoroquinolones in farming and veterinary science, there is a constant need in t ...

Wiley-Blackwell2014

From allergen detection to protein-ligand interactions : novel mass spectrometry-based analytical methods

Natalia Gasilova

Fundamental biological and biochemical studies, proteomics, practical nutrition-related and medical applications, all the research fields, where bioanalytical chemistry is an essential tool, constantly require the improvement of existing techniques of bioa ...

EPFL2014

Proteins/peptides purification by a three-well OFFGEL electrophoresis with immobilized ultra narrow pH gradient gels

Hubert Girault, Liang Qiao, Fernando Cortes Salazar, Elena Tobolkina

Purification and desalting of protein and peptide samples by a three-well OFFGEL electrophoresis with immobilized ultra narrow pH gradient gels is proposed as a fast preparative strategy for proteomics. The gist of this strategy is to separate the proteins ...

Royal Society of Chemistry2014

Electrostatic Spray Ionization Mass Spectrometry Imaging

Hubert Girault, Horst Vogel, Horst Pick, Andreas Stephan Lesch, Liang Qiao, Alexandra Bondarenko, Elena Tobolkina, Xiaoqin Zhong

Imaging samples on a surface by mass spectrometry (MS) requires the combination of MS detection with a scanning mode that enables localized desorption and ionization and/or detection of sample analytes with good spatial resolution. We have developed a new ...

Amer Chemical Soc2014