Publications related to Plant tissue culture

A satellite DNA array barcodes chromosome 7 and regulates totipotency via ZFP819

Didier Trono, Gabriela Ecco, Helen Mary Rowe

Mammalian genomes are a battleground for genetic conflict between repetitive elements and KRAB-zinc finger proteins (KZFPs). We asked whether KZFPs can regulate cell fate by using ZFP819, which targets a satellite DNA array, ZP3AR. ZP3AR coats megabase reg ...

2022

TRIM37 prevents formation of centriolar protein assemblies by regulating Centrobin

Pierre Gönczy, Coralie Busso, Benita Wolf, Fernando Romero Balestra, Tessa Vivianne Averink, Alizée Buff

TRIM37 is an E3 ubiquitin ligase mutated in Mulibrey nanism, a disease with impaired organ growth and increased tumor formation. TRIM37 depletion from tissue culture cells results in supernumerary foci bearing the centriolar protein Centrin. Here, we chara ...

2021

Phenotypic plasticity versus ecotypic differentiation under recurrent summer drought in two drought-tolerant pine species

Christoph Bachofen

Despite worldwide reports of high tree mortality, growing evidence indicates that many tree species are well adapted to survive repeated dry spells. The drought resilience of trees is related to their phenotypic plasticity and ecotypic differentiation. Whe ...

WILEY2021

Silk Hydrogels as Soft Substrates for Neural Tissue Engineering

Jeffrey Alan Hubbell, Federico Tortelli, Amy Marie Hopkins

There is great need for soft biomaterials that match the stiffness of human tissues for tissue engineering and regeneration. Hydrogels are frequently employed for extracellular matrix functionalization and to provide appropriate mechanical cues. It is chal ...

Wiley-V C H Verlag Gmbh2013

Malachite Green Interferes with Postantibiotic Recovery of Mycobacteria

John McKinney, Neeraj Dhar, Ekaterina Gelman

The genus Mycobacterium comprises slow-growing species with generation times ranging from hours to weeks. The protracted incubation time before colonies appear on solid culture medium can result in overgrowth by faster-growing microorganisms. To prevent co ...

2012

Spindle positioning in human cells relies on proper centriole formation and on the microcephaly proteins CPAP and STIL

Pierre Gönczy, Debora Keller, Petr Strnad, Fernando Romero Balestra, Gregor Kohlmaier

Patients with MCPH (autosomal recessive primary microcephaly) exhibit impaired brain development, presumably due to the compromised function of neuronal progenitors. Seven MCPH loci have been identified, including one that encodes centrosome protein 4.1 as ...

Company of Biologists2011

Hydrophilic Nanogels : Characterization and Behavior in Biological Environment

Catherine Ann Schütz

Hydrophilic nanocarriers, formed by electrostatic interaction between their oppositely charged components, are recognized as nanomaterials with an enormous potential as vectors in biomedical and pharmaceutical applications. However, comprehensive informati ...

EPFL2011

3D collagen cultures under well-defined dynamic strain: A novel strain device with a porous elastomeric support

Melody Swartz, Alice Anna Tomei, Federica Boschetti

The field of mechanobiology has grown tremendously in the past few decades, and it is now well accepted that dynamic stresses and strains can impact cell and tissue organization, cell-cell and cell-matrix communication, matrix remodeling, cell proliferatio ...

Wiley-Blackwell2009

Cell culture imaging using microimpedance tomography

Philippe Renaud, Olof Pontus Linderholm

We present a novel, inexpensive, and fast microimpedance tomography system for two-dimensional imaging of cell and tissue cultures. The system is based on four-electrode measurements using 16 planar microelectrodes (5 mum x 4 mm) integrated into a culture ...

2008

Induction of A9 dopaminergic neurons from neural stem cells improves motor function in an animal model of Parkinsons disease

Romain Zufferey

Neural stem cells (NSCs) are widely endorsed as a cell source for replacement strategies in neurodegenerative disease. However, their usefulness is currently limited by the inability to induce specific neurotransmitter phenotypes in these cells. In order t ...

Oxford University Press2008

Bipolar resistivity profiling of 3D tissue culture

Philippe Renaud, Yann Barrandon, Olof Pontus Linderholm, Jonas Vannod

We describe a new low-cost technique for continuous monitoring of the thickness of biofilms and tissue cultures, and we demonstrate the advantage of using electrodes of different dimensions to probe different depths of a sample. We have used electric imped ...

Elsevier2007

In vitro breeding of Brassica juncea L. to enhance metal accumulation and extraction properties

Jean-Paul Schwitzguebel, Erika Nehnevajova

In vitro breeding and somaclonal variation were used as tools to improve the potential of Indian mustard (Brassica juncea L.) to extract and accumulate toxic metals. Calli from B. juncea were cultivated on a modified MS medium supplemented with 10–200 μM C ...

2007

Two-dimensional microimpedance imaging for cell culture monitoring

Olof Pontus Linderholm

This thesis presents the fabrication and design of an impedance imaging sensor, and its application to monitoring in-vitro skin culture. Three-dimensional tissue cultures are becoming increasingly important in biological and medical research. There is, how ...

EPFL2006

Two-dimensional impedance imaging of cell migration and epithelial stratification

Philippe Renaud, Yann Barrandon, Thomas Michael Braschler, Olof Pontus Linderholm, Jonas Vannod

We present a miniaturized impedance imaging system, developed for 2D imaging of cell and tissue culture. The system is based on 16 microelectrodes (5 µm × 4 mm). An equivalent circuit for four-point (tetrapolar) impedance spectra was developed and validate ...

2006

Non-GMO approach for the improvement of heavy metal accumulation and extraction of high yielding crop species for efficient phytoextraction of contaminated soil

Erika Nehnevajova

Some plants able to take up heavy metals from contaminated soils offer a possibility to clean up sites contaminated with heavy metals. Plants thus act as a solar driven pump, which can extract and concentrate heavy metals from the environment. Since most o ...

EPFL2005