Publications related to Cell polarity

Universal Casimir attraction between filaments at the cell scale

The electromagnetic Casimir interaction between dielectric objects immersed in salted water includes a universal contribution that is not screened by the solvent and therefore long-ranged. Here, we study the geometry of two parallel dielectric cylinders. W ...

Iop Publishing Ltd2024

Investigating the bioaccumulation potential of anionic organic compounds using a permanent rainbow trout liver cell line

Kristin Schirmer, Juliane Hollender, Fabian Gerhard Peter Balk

Permanent rainbow trout (Oncorhynchus mykiss) cell lines represent potential in vitro alternatives to experiments with fish. We here developed a method to assess the bioaccumulation potential of anionic organic compounds in fish, using the rainbow trout li ...

PERGAMON-ELSEVIER SCIENCE LTD2023

Dissecting centriole fate in C. elegans development

Nils Kalbfuss

The presence of centrioles is critical for fundamental cell and developmental processes, including polarity, migration and division. Although centrioles are present in most proliferating cells, they are thought to vanish after cell cycle exit in some diffe ...

EPFL2022

Characterization of Pseudomonas aeruginosa mechanosensing through label-free imaging of type IV pili

Lorenzo Anton-Louis Talà

Bacteria are ubiquitously found in all sorts of environment. They're found in the ocean, soil, or even in our guts or on our skin. Independently of their niche, they can transition from a planktonic state were they freely swim in an aqueous environment to ...

EPFL2022

Analysis and Modeling of the Dynamics of Traction Forces during Cell Polarization and their Role in the Organization of Edge Activity

Zeno Messi

The capacity to break symmetry and organize activity to move directionally is a fundamental property of eukaryotic cells. To explain the organization of cell-edge activity, models commonly rely on front-to-back gradients of functional components or regulat ...

EPFL2021

Self-organized patterning of cell morphology via mechanosensitive feedback

Marko Popovic

Tissue organization is often characterized by specific patterns of cell morphology. How such patterns emerge in developing tissues is a fundamental open question. Here, we investigate the emergence of tissue-scale patterns of cell shape and mechanical tiss ...

2021

Expression of E-Cadherin in Epithelial Cancer Cells Increases Cell Motility and Directionality through the Localization of ZO-1 during Collective Cell Migration

Hwanseok Jang

Collective cell migration of epithelial tumor cells is one of the important factors for elucidating cancer metastasis and developing novel drugs for cancer treatment. Especially, new roles of E-cadherin in cancer migration and metastasis, beyond the epithe ...

MDPI2021

The influence of the crowding assumptions in biofilm simulations

Vassily Hatzimanikatis, Liliana Angeles Martinez

Author summary In nature, many organisms grow in crowded biofilms that protect against stressful conditions, making their control/eradication a challenge. Modeling these microbial systems is a valuable tool for studying the interactions among cells and exp ...

2021

Engineered Extracellular Matrices with Integrated Wireless Microactuators to Study Mechanobiology

Mahmut Selman Sakar, Erik Mailand, Nikolaos Bouklas, Fazil Emre Uslu

Mechanobiology explores how forces regulate cell behaviors and what molecular machinery are responsible for the sensing, transduction, and modulation of mechanical cues. To this end, probing of cells cultured on planar substrates has served as a primary ex ...

Wiley2021

GRASP55 regulates intra-Golgi localization of glycosylation enzymes to control glycosphingolipid biosynthesis

Giovanni D'Angelo, Charlotte Julie Caroline Gehin, Laura Capolupo, Riccardo Rizzo

The Golgi apparatus, the main glycosylation station of the cell, consists of a stack of discontinuous cisternae. Glycosylation enzymes are usually concentrated in one or two specific cisternae along the cis-trans axis of the organelle. How such compartment ...

WILEY2021

Receptor-induced transient responses in cells with oscillatory actin dynamics

Jose Negrete Jr

Living cells adjust their sensing and migratory machinery in response to changes in their environment. In this work, we show that cells of the social amoeba Dictyostelium discoideum modulate the dynamical state of their actin cytoskeleton in response to an ...

AMER PHYSICAL SOC2020

Ligand Binding to the Collagen VI Receptor Triggers a Talin-to-RhoA Switch that Regulates Receptor Endocytosis

Françoise Gisou van der Goot Grunberg, Matteo Dal Peraro, Laurence Gouzi Abrami, Luciano Andres Abriata, Béatrice Kunz, Shixu Yan

Capillary morphogenesis gene 2 (CMG2/ANTXR2) is a cell surface receptor for both collagen VI and anthrax toxin. Biallelic loss-of-function mutations in CMG2 lead to a severe condition, hyaline fibromatosis syndrome (HFS). We have here dissected a network o ...

Cell Press - Elsevier2020

A biphasic growth model for cell pole elongation in mycobacteria

Georg Fantner, John McKinney, Haig Alexander Eskandarian, Mélanie Thérèse Marie Hannebelle, Chiara Toniolo, Joëlle Xiao Yuan Ven, Gaëlle Madeleine Vuaridel

Mycobacteria grow by inserting new cell wall material in discrete zones at the cell poles. This pattern implies that polar growth zones must be assembled de novo at each division, but the mechanisms that control the initiation of new pole growth are unknow ...

Nature Research2020

Outer membrane lipoprotein NlpI scaffolds peptidoglycan hydrolases within multi-enzyme complexes in Escherichia coli

Ann-Kristin Hov

The peptidoglycan (PG) sacculus provides bacteria with the mechanical strength to maintain cell shape and resist osmotic stress. Enlargement of the mesh-like sacculus requires the combined activity of peptidoglycan synthases and hydrolases. In Escherichia ...

WILEY2020

Traction Forces Control Cell-Edge Dynamics and Mediate Distance Sensitivity during Cell Polarization

Alexander Verkhovskiy, Franck Claude Raynaud, Alicia Carine Bornert, Zeno Messi

Traction forces are generated by cellular actin-myosin system and transmitted to the environment through adhesions. They are believed to drive cell motion, shape changes, and extracellular matrix remodeling [1 -3] . However, most of the traction force anal ...

2020

Unequal cleavage of the C. elegans zygote ensures robust embryogenesis

Radek Jankele

Asymmetric cell divisions play key roles in generating cellular diversity during embryogenesis, and in tissue homeostasis during postembryonic life. Particular asymmetric cell divisions producing differentially sized daughter cells were described in many s ...

EPFL2020

Aurora A depletion reveals centrosome-independent polarization mechanism in Caenorhabditis elegans

Pierre Gönczy, Coralie Busso, Kerstin Klinkert Fabbri, Lukas von Tobel, Christian Gentili, Marie Juliette Ségolène Pierron, Sarah Herrman

How living systems break symmetry in an organized manner is a fundamental question in biology. In wild-type Caenorhabditis elegans zygotes, symmetry breaking during anterior-posterior axis specification is guided by centrosomes, resulting in anterior-direc ...

2019

Dielectrophoresis‐assisted creation of cell aggregates under flow conditions using planar electrodes

Philippe Renaud, Harald Van Lintel, Jonathan Bastien Cottet, Soufian Lasli

We present a microfluidic platform allowing dielectrophoresis-assisted formation of cell aggregates of controlled size and composition under flow conditions. When specific ex- perimental conditions are met, negative dielectrophoresis allows efficient conce ...

2019

Multi-phosphorylation reaction and clustering tune Pom1 gradient mid-cell levels according to cell size

Suliana Manley, Anna Archetti, Lina Carlini, Charlotte Sophia Floderer, Thaïs Reichler

Protein concentration gradients pattern developing organisms and single cells. In Schizosaccharomyces pombe rod-shaped cells, Pom1 kinase forms gradients with maxima at cell poles. Pom1 controls the timing of mitotic entry by inhibiting Cdr2, which forms s ...

2019

Probing the distribution of cortical components, including PtdIns(4,5)P2, during asymmetric division of C. elegans embryos

Melina Jasmin Scholze

Asymmetric cell division is crucial for embryonic development and stem cell lineages. In the one-cell C. elegans embryo, a contractile cortical actomyosin network contributes to asymmetric division by segregating PAR proteins to discrete cortical domains. ...

EPFL2018