Concept

Binding selectivity

Related publications (27)

Probing Odorant-Mediated Receptor Activation

Molecular interactions of odorants with their olfactory receptors (ORs) are of central importance for the mammalian olfactory system to detect and discriminate a large variety of odors with a limited set of receptors. How a particular OR binds and distingu ...

EPFL2012

Metal-based Chemosensors for Carbohydrates and Biologically Important Ligands

Vera Janowski

Chemosensors based on indicator displacement were developed for the optical detection of different analytes in aqueous solution. The sensing of carbohydrates, including glucose, in aqueous solution at pH 7.4 was achieved by a fluorescent indicator displace ...

EPFL2012

Automated design of ligands to polypharmacological profiles

Daniel Constam

The clinical efficacy and safety of a drug is determined by its activity profile across many proteins in the proteome. However, designing drugs with a specific multi-target profile is both complex and difficult. Therefore methods to design drugs rationally ...

Nature Publishing Group2012

Semisynthetic Fluorescent Sensor Proteins Based on Self-Labeling Protein Tags

Matthias Albert Brun

Genetically encoded fluorescent sensor proteins offer the possibility to probe the concentration of key metabolites in living cells. The approaches currently used to generate such fluorescent sensor proteins lack generality, as they require a protein that ...

EPFL2011

Metal-Based Chemosensors for Important Bioanalytes

Sébastien Rochat

This work describes different strategies for the detection of biologically relevant analytes. The main goal was to achieve sensing in water, which is a prerequisite for application to real biological samples. In several cases, we could demonstrate that our ...

EPFL2010

O6-alkylguanine-DNA alkyltransferase as a new tool for functional proteomics

Anke Arnold

Recently the sequencing project of the human genome has been completed. The interest of the post-sequencing-era is shifting nowadays towards the investigation of the biological function and localization of the encoded proteins. Proteins are essential parts ...

EPFL2007

Reversible sequential-binding probe receptor-ligand interactions in single cells

Horst Vogel, Rudolf Hovius, Jean-Manuel Segura, Marinela Gjoni Boillat, Christoph Schreiter

With the reversible sequential (ReSeq) binding assay, we present a novel approach for the ultrasensitive profiling of receptor function in single living cells. This assay is based on the repetitive application of fluorescent ligands that have fast assocn.- ...

2005