Publications related to Photobleaching

NIR Fluorescence lifetime macroscopic imaging with a time-gated SPAD camera

Edoardo Charbon, Claudio Bruschini, Arin Can Ülkü

The performance of SwissSPAD2 (SS2), a large scale, widefield time-gated CMOS SPAD imager developed for fluorescence lifetime imaging, has recently been described in the context of visible range and fluorescence lifetime imaging microscopy (FLIM) of dyes w ...

SPIE-INT SOC OPTICAL ENGINEERING2022

Temporal resolution doubling in fluorescence light-sheet microscopy via a hue-encoded shutter and regularization

Michael Stefan Daniel Liebling, Christian Jaques

Studying dynamic biological processes, such as heart development and function in zebrafish embryos, often relies on multi-channel fluorescence labeling to distinguish multiple anatomical features, yet also demands high frame rates to capture rapid cell mot ...

2020

Fluorescence lifetime imaging with a megapixel SPAD camera and neural network lifetime estimation

Edoardo Charbon, Claudio Bruschini, Ming-Lo Wu, Kazuhiro Morimoto

Fluorescence lifetime imaging microscopy (FLIM) is a key technology that provides direct insight into cell metabolism, cell dynamics and protein activity. However, determining the lifetimes of different fluorescent proteins requires the detection of a rela ...

NATURE RESEARCH2020

Interference of engineered nanomaterials in flow cytometry: A case study

Aurélie Walter

Nanotechnology is regarded as the enabling technology of the 21st century. However, only a relatively small number of nano-enabled medical and healthcare products finally made their way to the market. There are several reasons why such innovative approache ...

ELSEVIER SCIENCE BV2018

Review of the role played by the photosensitizer's photobleaching during photodynamic therapy

Georges Wagnières, Matthieu Philippe Emmanuel Zellweger

Photodynamic therapy (PDT) is based on the combined administration of photosensitizing drugs and light to treat a variety of malignant, pre-malignant and also non-malignant diseases. The reactive species resulting from the excitation of the photosensitizer ...

Natl Inst Optoelectronics2016

Intramolecular Distances and Dynamics from the Combined Photon Statistics of Single-Molecule FRET and Photoinduced Electron Transfer

Luc Reymond

Single-molecule Forster resonance energy transfer (FRET) and photoinduced electron transfer (PET) have developed into versatile and complementary methods for probing distances and dynamics in biomolecules. Here we show that the two methods can be combined ...

Amer Chemical Soc2013

Assessment of transferrin recycling by Triplet Lifetime Imaging in living cells

Theo Lasser, Harm-Anton Klok, Zuzana Kadlecova

An optical method is presented that allows the measurement of the triplet lifetime of a fluorescent molecule. This is a characteristic specific to each fluorophore. Based on differences in triplet lifetimes of two fluorescent species (autofluorescence vers ...

Optical Soc Amer2012

Development of Minimally-Invasive Optical Methods to Individualize the Doses Used for Therapeutic Applications of Light

Filippo Piffaretti

Despite the experience gained over several decades in various types of light-based medical treatments, the optimization of the corresponding therapeutic protocols and accurate forecasting of their outcome have not yet been achieved in many cases. The diffi ...

EPFL2010

Selective Visualization of Fluorescent Sterols in Caenorhabditis elegans by Bleach-Rate-Based Image Segmentation

Daniel Sage

The nematode Caenorhabditis elegans is a genetically tractable model organism to investigate sterol transport. In vivo imaging of the fluorescent sterol, dehydroergosterol (DHE), is challenged by C. elegans' high autofluorescence in the same spectral regio ...

Wiley InterScience2010

Single molecule detection on surfaces

Marcel Leutenegger

Monitoring biological relevant reactions on the single molecule level based on fluorescence spectroscopy techniques has become one of the most promising approaches for understanding a variety of phenomena in biophysics, biochemistry and life science. By ap ...

EPFL2007

Theory of molecular excitation and relaxation near a plasmonic device

Olivier Martin, Thierry Laroche

The new optical concepts currently developed in the research field of plasmonics can have significant practical applications for integrated optical device miniaturization as well as for molecular sensing applications. Particularly, these new devices can of ...

2007

Dynamics of the 5-HT3A serotonin receptor in living cells

Emmanuel Guignet

The 5-HT3 serotonin receptor belongs to the cys-loop ligand-gated ion channel (LGIC) family. It is located in the central and peripheral nervous system and is implicated in certain pathologic situations. The 5-HT3 receptor is investigated here, as a typica ...

EPFL2006

PixFRET, an ImageJ Plug-in for FRET Calculation That Can Accommodate Variations in Spectral Bleed-throughs

Daniel Sage, Walter Wahli

Fluorescence resonance energy transfer (FRET) allows the user to investigate interactions between fluorescent partners. One crucial issue when calculating sensitized emission FRET is the correction for spectral bleed-throughs (SBTs), which requires to calc ...

Wiley-Liss2005