Publications related to Negative stain

Identification of a Dps contamination in Mitomycin-C–induced expression of Colicin Ia

Colicins are bacterial toxins targeting Gram-negative bacteria, including E. coli and related Enterobacteriaceae strains. Some colicins form ion-gated pores in the inner membrane of attacked bacteria that are lethal to their target. Colicin Ia was the firs ...

2021

Picture of the wet electron: a localized transient state in liquid water

Alfredo Pasquarello, Francesco Ambrosio, Michele Pizzochero

A transient state of the excess electron in liquid water preceding the development of the solvation shell, the so-called wet electron, has been invoked to explain spectroscopic observations, but its binding energy and atomic structure have remained highly ...

2019

A Cell Atlas for the Mouse Brain

Henry Markram, Daniel Keller, Marc-Oliver Gewaltig

Despite vast numbers of studies of stained cells in the mouse brain, no current brain atlas provides region-by-region neuron counts. In fact, neuron numbers are only available for about 4% of brain of regions and estimates often vary by as much as 3-fold. ...

2018

Controlled Charging of Ferroelastic Domain Walls in Oxide Ferroelectrics

Tomas Sluka, Li Jin, Ludwig Feigl, Barbara Maraston Fraygola, Xiankui Wei

Conductive domain walls (DWs) in ferroic oxides as device elements are a highly attractive research topic because of their robust and agile response to electric field. Charged DWs possessing metallic-type conductivity hold the highest promises in this aspe ...

Amer Chemical Soc2017

Height-resolved quantification of microstructure and texture in polycrystalline thin films using TEM orientation mapping

Cécile Hébert, Duncan Thomas Lindsay Alexander, Arthur Brian Aebersold

A method is presented for the quantitative investigation of microstructure and texture evolution in polycrystalline thin films based on in-plane automated crystal orientation mapping in transmission electron microscopy, from the substrate up. To demonstrat ...

Elsevier Science Bv2015

Focused light delivery and all optical scanning from a multimode optical fiber using digital phase conjugation

Demetri Psaltis, Christophe Moser, Salma Farahi

We demonstrate a digital phase conjugation technique for generating a sharp focus point at the end of a multimode optical fiber. A sharp focus is experimentally obtained at the distal end of a 200 mu m core multimode fiber. By recording the digital hologra ...

Spie-Int Soc Optical Engineering2013

Transmission Electron Microscopy and Nanoindentation Studies of Ultrathin Transition Metal Dichalcogenide Semiconductor Membranes

Jacopo Brivio

Until now, only two intrinsically two-dimensional materials have been isolated: graphene and boron nitride (BN). As a consequence, the knowledge of two-dimensional systems is still limited and other classes of two-dimensional materials are needed to enrich ...

EPFL2013

Dynamic measurement of the height and volume of migrating cells by a novel fluorescence microscopy technique

Jean-Jacques Meister, Chiara Gabella, Alexander Verkhovskiy, Lara Buscemi Estefanell, Benoît Vianay

We propose a new technique to measure the volume of adherent migrating cells. The method is based on a negative staining where a fluorescent, non-cell-permeant dye is added to the extracellular medium. The specimen is observed with a conventional fluoresce ...

2011

Metal-related gate sinking due to interfacial oxygen layer in Ir/InAlN high electron mobility transistors

Nicolas Grandjean, Jean-François Carlin, Marcus Gonschorek

We report on an annealing-induced "gate sinking" effect in a 2-nm-thin In0.17Al0.83N/AlN barrier high electron mobility transistor with Ir gate. Investigations by transmission electron microscopy linked the effect to an oxygen containing interlayer between ...

2010

Effects of base-pair sequence, nicks and gaps on DNA minicircle shapes : analysis and experiment

Arnaud Amzallag

DNA is a long polymer with the form of a double helix of about two nanometers diameter (2.10-9 meters). It is composed of nucleotides whose sequence carries the information of heredity. The four possible nucleotides have slightly different geometries, and ...

EPFL2008