Publications related to Osteoblast

Synergetic Support of Cartilage Homeostasis via Coupled Thermal-Hydrostatic Pressure Stimuli

Cartilage homeostasis, crucial for musculoskeletal function, is orchestrated by interconnected biophysical cues. In healthy cartilage, repetitive compressive loading not only elicits a range of mechanical stimuli but also induces a gradual transient temper ...

EPFL2024

Enhancing Chondrocyte Biosynthetic Activities: Harnessing the Power of Loading-Induced Evolved Temperature

Theofanis Stampoultzis

Chondrocytes within articular cartilage possess the capacity to perceive and react to signals generated by the typical load-bearing actions of daily life, such as walking or running. Articular cartilage is predominantly oriented toward compression-based fu ...

EPFL2024

Unraveling cartilage degeneration through synergistic effects of hydrostatic pressure and biomimetic temperature increase

Dominique Pioletti, Peyman Karami, Naser Nasrollahzadeh Mamaghani, Theofanis Stampoultzis, Yanheng Guo, Vijay Kumar Rana

Cartilage degeneration, typically viewed as an irreversible, vicious cycle, sees a significant reduction in two essential biophysical cues: the well-established hydrostatic pressure (HP) and the recently discovered transient temperature increase. Our study ...

Cell Press2023

Biological responses at the interface of Ti-doped diamond-like carbon surfaces for indoor environment application

Sami Rtimi

Diamond-like carbon (DLC) and titanium-doped DLC coatings were prepared by hybrid PECVD/direct current magnetron sputtering (DCMS). In this study, we show that the operating conditions of titanium-doped DLC coatings used for implants in surgical devices si ...

SPRINGER HEIDELBERG2020

Genetically encoded betaxanthin-based small-molecular fluorescent reporter for mammalian cells

Leo Scheller, Karl Gademann, Simon Sieber

We designed and engineered a dye production cassette encoding a heterologous pathway, including human tyrosine hydroxylase and Amanita muscaria 4,5-DOPA dioxygenase, for the biosynthesis of the betaxanthin family of plant and fungal pigments in mammalian c ...

2020

From the product to the process: Fine-scale skeletal structures of scleractinian corals and their relevance to biomineralization models, geochemical sampling strategies, and phylogenetic reconstructions

Anders Meibom, Jaroslaw Hubert Stolarski

Interpretation of biomineralization processes based solely on the end product - the skeleton - may seem abreakneck task. This is because the biologically controlled mineralization involves cascades of intermediatephases and the activity of a myriad of orga ...

2019

Quantitative intrinsic auto-cathodoluminescence can resolve spectral signatures of tissue-isolated collagen extracellular matrix

Peter Frey, Jeffrey Alan Hubbell, Hans Mattias Larsson, Elif Vardar, Marcin Stefan Zielinski, Ganesh Vythilingam

By analyzing isolated collagen gel samples, we demonstrated in situ detection of spectrally deconvoluted auto-cathodoluminescence signatures of specific molecular content with precise spatial localization over a maximum field of view of 300 mu m. Correlati ...

2019

Alignment of collagen fiber in knitted silk scaffold for functional massive rotator cuff repair

Dominique Pioletti, Weiliang Shen, Ting Zhu, Jiayun Huang

Rotator cuff tear is one of the most common types of shoulder injuries, often resulting in pain and physical debilitation. Allogeneic tendon-derived decellularized matrices do not have appropriate pore size and porosity to facilitate cell infiltration, whi ...

Elsevier2017

Scleractinian coral morphogenesis : searching for stem cells and precursors of the skeletogenic calicodermis

Agathe Eliette Marie Lecointe

Metamorphosis is a crucial step in the life cycle of a scleractinian coral: The swimming coral larva, the planula, settles, and metamorphoses into a sessile calcifying primary polyp, which subsequently grows into an adult colony. Importantly, morphogenesis ...

EPFL2016

Improving Osteoblast Response In Vitro by a Nanostructured Thin Film with Titanium Carbide and Titanium Oxides Clustered around Graphitic Carbon

Giovanni Longo

Introduction Recently, we introduced a new deposition method, based on Ion Plating Plasma Assisted technology, to coat titanium implants with a thin but hard nanostructured layer composed of titanium carbide and titanium oxides, clustered around graphitic ...

Public Library of Science2016

Tubular Compressed Collagen Scaffolds for Ureteral Tissue Engineering in a Flow Bioreactor System

Jeffrey Alan Hubbell, Peter Frey, Hans Mattias Larsson, Elif Vardar, Kalitha Pinnagoda, Mouti Elodie Mouloungui

Ureteral replacement by tissue engineering might become necessary following tissue loss after excessive ureteral trauma, after retroperitoneal cancer, or even after failed reconstructive surgery. This need has driven innovation in the design of novel scaff ...

Mary Ann Liebert, Inc2015

Variability of the pullout strength of cancellous bone screws with cement augmentation

Dominique Pioletti

Background: orthopaedic surgeons often face clinical situations where improved screw holding power in cancellous bone is needed. Injectable calcium phosphate cements are one option to enhance fixation. Methods: paired screw pullout tests were undertaken in ...

Elsevier2015

Compounds for functionalizing biomaterials

Sandrine Gerber, Françoise Borcard

The present invention provides compounds suitable for functionalizing biomaterials, in particular prosthetics and implant materials, functionalized biomaterials, and methods for synthesizing the compound. The compounds contain an anchoring group, a group f ...

2014

Differential Roles of MAPK Kinases MKK3 and MKK6 in Osteoclastogenesis and Bone Loss

Mario Michael Zaiss

Bone mass is maintained by osteoclasts that resorb bone and osteoblasts that promote matrix deposition and mineralization. Bone homeostasis is altered in chronic inflammation as well as in post-menopausal loss of estrogen, which favors osteoclast activity ...

Public Library of Science2014

T Cell Costimulation Molecules CD80/86 Inhibit Osteoclast Differentiation by Inducing the IDO/Tryptophan Pathway

Mario Michael Zaiss, Zhen Chen

Bone resorption is seminal for the physiological remodeling of bone during life. However, this process needs to be strictly controlled; excessive bone resorption results in pathologic bone loss, osteoporosis, and fracture. We describe a control mechanism o ...

Amer Assoc Advancement Science2014

Osteoconductive surfaces generated from peptide functionalized poly(2-hydroxyethyl methacrylate-co-2-(methacryloyloxy)ethyl phosphate) brushes

Harm-Anton Klok, Dusko Paripovic

Modification of bone implant materials with biofunctional surfaces that facilitate osteoblast adhesion and promote matrix mineralization is an attractive strategy to improve osseointegration. This manuscript discusses the preparation and properties of RGD ...

Royal Society of Chemistry2012

Functionalization of microstructured open-porous bio-ceramic scaffolds with human fetal cells

Sandrine Gerber, Corinne Scaletta, Davide Städler, Françoise Borcard

Bone substitute materials allowing trans-scaffold migration and in-scaffold survival of human bone-derived cells are mandatory for development of cell-engineered permanent implants to repair bone defects. In this study, we evaluated the influence on human ...

American Chemical Society2012

Injectable calcium phosphate cement for augmentation around cancellous bone screws. In vivo biomechanical studies

Dominique Pioletti, Vincent Stadelmann

In lower cancellous apparent bone density, it can be difficult to achieve adequate screw fixation and hence stable fracture fixation. Different strategies have been proposed, one of them is through augmentation using calcium phosphate cement in the region ...

Elsevier2012

26Mg labeling of the sea urchin regenerating spine: Insights into echinoderm biomineralization process

Anders Meibom, Christophe Kopp, Jaroslaw Hubert Stolarski

This paper reports the results of the first dynamic labeling experiment with regenerating spines of sea urchins Paracentrotus lividus using the stable isotope Mg-26 and NanoSIMS high-resolution isotopic imaging, which provide a direct information about the ...

Elsevier2011

High-density collagen gel tubes as a matrix for primary human bladder smooth muscle cells

Jeffrey Alan Hubbell, Peter Frey, Lionel Ambroise Micol

Tissue-engineered grafts for the urinary tract are being investigated for the potential treatment of several urologic diseases. These grafts, predominantly tubular-shaped, usually require in vitro culture prior to implantation to allow cell engraftment on ...

Elsevier2011