Publications related to Viroplasm

Stimulus-responsive assembly of nonviral nucleocapsids

Controlled assembly of a protein shell around a viral genome is a key step in the life cycle of many viruses. Here we report a strategy for regulating the co-assembly of nonviral proteins and nucleic acids into highly ordered nucleocapsids in vitro. By fus ...

Nature Portfolio2024

Dynamic Nuclear Polarization Magic-Angle Spinning Nuclear Magnetic Resonance Combined with Molecular Dynamics Simulations Permits Detection of Order and Disorder in Viral Assemblies

David Lyndon Emsley, Marc Anthony Caporini

We report dynamic nuclear polarization (DNP)-enhanced magic-angle spinning (MAS) NMR spectroscopy in viral capsids from HIV-1 and bacteriophage AP205. Viruses regulate their life cycles and infectivity through modulation of their structures and dynamics. W ...

2019

A Quantitative Approach to Evaluate the Impact of Fluorescent Labeling on Membrane-Bound HIV-Gag Assembly by Titration of Unlabeled Proteins

Suliana Manley, Romain Wyss, Julia Gunzenhäuser

The assembly process of the human immunodeficiency virus 1 (HIV-1) is driven by the viral polyprotein Gag. Fluorescence imaging of Gag protein fusions is widely performed and has revealed important information on viral assembly. Gag fusion proteins are com ...

Public Library Science2014

Virus Inactivation during Water Treatment : the Role of Virus Aggregation and the Disinfection Potential of Sunlight

Michael Jon Mattle

Virus removal and inactivation is still a major challenge for water treatment facilities in both industrialised nations and developing countries. This may seem surprising as chlorine disinfection started to spread broadly over a century ago. However, many ...

EPFL2013

Inactivation and tailing during UV254 disinfection of viruses: contributions of viral aggregation, light shielding within viral aggregates and recombination

Tamar Kohn, Michael Jon Mattle

UV disinfection of viruses frequently leads to tailing after an initial exponential decay. Aggregation, light shielding, recombination or resistant virus sub-populations were proposed as explanations; however, none of these options has conclusively been de ...

Amer Chemical Soc2012

Photoinactivation of virus on iron-oxide coated sand: enhancing inactivation in sunlit waters

Tamar Kohn, Brian Pecson, Loïc Decrey

Adsorption onto iron oxides can enhance the removal of waterborne viruses in constructed wetlands and soils. If reversible adsorption is not coupled with inactivation, however, infective viruses may be released when changes in solution conditions cause des ...

Elsevier2012

Impact of virus aggregation on inactivation by peracetic acid and implications for other disinfectants

Tamar Kohn, Paolo Perona, Benoît Crouzy, Michael Jon Mattle, Krista Rule Wigginton, Moritz Brennecke

Viruses in wastewater and natural environments are often present as aggregates. The disinfectant dose required for their inactivation, however, is typically determined with dispersed viruses. This study investigates how aggregation affects virus inactivati ...

2011

Tissue macrophages suppress viral replication and prevent severe immunopathology in an interferon-I-dependent manner in mice

Nicola Harris

The innate immune response plays an essential role in the prevention of early viral dissemination. We used the lymphocytic choriomeningitis virus model system to analyze the role of tissue macrophages/Kupffer cells in this process. Our findings demonstrate ...

2010