Publications related to Two-dimensional gel electrophoresis

Telomere protection against oxidative stress

Telomeres are nucleoprotein structures at the ends of linear chromosomes, being essential for the maintenance of genomic integrity. Telomeres have a unique structure which distinguishes chromosome termini from DNA damage sites. Shelterin complexes are the ...

EPFL2023

Site-Specific Protein Conjugation onto Fluorescent Single-Walled Carbon Nanotubes

Ardemis Anoush Boghossian, Shang-Jung Wu, Vitalijs Zubkovs, Nils Schürgers

Semiconducting single-walled carbon nanotubes (SWCNTs) are among the few photostable optical emitters that are ideal for sensing, imaging, drug delivery, and monitoring of protein activity. These applications often require strategies for immobilizing prote ...

2020

A smart low molecular weight gelator for the triple detection of copper (II), mercury (II), and cyanide ions in water resources

Rosario Scopelliti

Novel supramolecular gelators based on quinoline-indolin-2-one structure were synthesized. These gelators formed stable organogels in a mixture of DMSO/H2O (1:1). The scanning electron microscopy (SEM), IR and NMR spectroscopies, and rheological measuremen ...

ELSEVIER2020

Comparison of the functional properties of trimeric and monomeric CaiT of Escherichia coli

Kamila Krystyna Guérin

Secondary transporters exist as monomers, dimers or higher state oligomers. The significance of the oligomeric state is only partially understood. Here, the significance of the trimeric state of the L-carnitine/gamma-butyrobetaine antiporter CaiT of Escher ...

Nature Research2019

Equilibrium and Out-of-Equilibrium Adherence of Hydrogels against Polymer Brushes

Jennifer Mary Adrienne Alice Macron, Costantino Creton

At low pH, underwater adherence between poly(N,N-dimethylacrylamide) hydrogels and poly(acrylic acid) brushes is due to the formation of multiple hydrogen bonds. The effect of the key parameters controlling the formation of these interactions (contact time ...

American Chemical Society2018

Castles fall from inside: Evidence for dominant internal photo-catalytic mechanisms during treatment of Saccharomyces cerevisiae by photo-Fenton at near-neutral pH

César Pulgarin, Sami Rtimi, Stefanos Giannakis, Cristina del Socorro Lonfat

In this work, the antimicrobial effects of the photo-Fenton process on yeast cells were tested (Saccharomyces cerevisiae), an essential eukaryotic unicellular model of living cells. Near-neutral pH was used in all studies, while iron sulfate and iron citra ...

Elsevier2016

Investigation of cell death mechanisms in human lymphatic endothelial cells undergoing photodynamic therapy

Witold Waldemar Kilarski

Background: Photodynamic therapy (PDT) has been shown to induce ablation and functional occlusion of tumor-associated lymphatic vessels. However, direct effects of PDT on lymphatic endothelial cells (LECs) have not been studied so far. The aim of this stud ...

Elsevier Science Bv2016

Porous silica enhanced proteolysis during Off-Gel separation for efficient protein identification

Hubert Girault, Liang Qiao, Mohammad Reza Pourhaghighi

An advanced approach is developed in this work for simultaneous on-line separation and digestion of proteins by combining the Off-Gel isoelectric focusing (IEF) and enzymatic nanoreactor enhanced proteolysis. The nanoreactor was prepared by preloading tryp ...

Elsevier2015

Proteins/peptides purification by a three-well OFFGEL electrophoresis with immobilized ultra narrow pH gradient gels

Hubert Girault, Liang Qiao, Fernando Cortes Salazar, Elena Tobolkina

Purification and desalting of protein and peptide samples by a three-well OFFGEL electrophoresis with immobilized ultra narrow pH gradient gels is proposed as a fast preparative strategy for proteomics. The gist of this strategy is to separate the proteins ...

Royal Society of Chemistry2014

A four-component organogel based on orthogonal chemical interactions

Kay Severin, Kurt Schenk, Nicolas Luisier

A thermoresponsive organogel was obtained by orthogonal assembly of four compounds using dynamic covalent boronate ester and imine bonds, as well as dative boron-nitrogen bonds. It is shown that the gel state can be disrupted or reinforced by chemicals whi ...

Royal Society of Chemistry2014

New analytical tools combining gel electrophoresis and mass spectrometry

Elena Tobolkina

Proteomics has been one of the main projects challenging biological and analytical chemists for many years. The separation, identification and quantification of all the proteins expressed within biological systems remain the main objectives of proteomics. ...

EPFL2014

Coupling Isoelectric Focusing Gel Electrophoresis to Mass Spectrometry by Electrostatic Spray Ionisation

Hubert Girault, Liang Qiao, Elena Tobolkina

Gel electrophoresis has been used for decades as a high-resolution separation technique for proteins and protein isomers, but limited in the coupling with MS because of low throughput and poor automaticity compared with LC-MS. In this work, we have develop ...

American Chemical Society2013

Sedimentation of macroscopic rigid knots and its relation to gel electrophoretic mobility of DNA knots

Giovanni Dietler, Cédric Weber, Andrzej Stasiak, Mathias Carlen

We address the general question of the extent to which the hydrodynamic behaviour of microscopic freely fluctuating objects can be reproduced by macrosopic rigid objects. In particular, we compare the sedimentation speeds of knotted DNA molecules undergoin ...

Nature Publishing Group2013

The molecular mechanisms of antimetastatic ruthenium compounds explored through DIGE proteomics

Paul Joseph Dyson, Angela Casini

DIGE (difference in gel electrophoresis) proteomics is exploited here to gain insight into the molecular mechanisms of two established ruthenium-based antimetastatic agents, namely trans-[tetrachloro (DMSO) (imidazole)ruthenate(III)] (NAMI-A) and [Ru(eta(6 ...

Elsevier Science Inc2013

From food to physiology characterization and in vitro digestion of milk products and investigation of postprandial metabolism and inflammation after a high-fat meal intake in a human nutrition intervention study

Flurina Schwander

According to the World Health Organization worldwide obesity has more than doubled since 1980. The World health statistics 2012 report shows that one in six adults is obese, one in ten diabetic and one in three has a raised blood pressure. Overweight and o ...

EPFL2013

Development of Electrokinetically Based Two-Dimensional Separation Methodologies for Proteomic Studies

Mohammad Reza Pourhaghighi

Proteomic studies require analytical methods capable of separating, identifying and quantifying thousands of proteins. Consequently, developing separation methodologies with high resolving power necessary to separate complex protein samples prior to protei ...

EPFL2012

Human serum albumin binding and cytotoxicity studies of surfactant–cobalt(III) complex containing 1,10-phenanthroline ligand

Hubert van den Bergh, Georges Wagnières

The characteristics of the binding reaction of surfactant-cobalt(III) complex, cis-[Co(phen)(2)(C14H29NH2)]Cl-2 center dot 3H(2)O (phen =1,10-phenanthroline, C14H29NH2 = tetradecylamine) with human serum albumin (HSA) were studied by fluorescence and UV-vi ...

Elsevier2011

Metabolization of [Ru(η6-C6H5CF3)(pta)Cl2]: a cytotoxic RAPTA-type complex with a strongly electron withdrawing arene ligand

Paul Joseph Dyson, Anna Katherine Renfrew, Christian Hartinger

The anticancer ruthenium-arene compd. [Ru(η6-C6H5CF3)(pta)Cl2] (pta = 1,3,5-triaza-7-phosphatricyclo[3.3.1.1]decane), termed RAPTA-CF3, with the electron-withdrawing α,α,α-trifluorotoluene ligand, is one of the most cytotoxic RAPTA compds. known. To ration ...

Springer-Verlag2010

Transient Gene Expression in HEK-293E Cells for Recombinant Protein Production : Process Characterization towards GMP Approval

Sophie Nallet

The growing demand of biopharmaceutical products is boosting the market for therapeutic recombinant proteins (r-proteins). More than half of the 140 r-proteins that have gained approval for human therapeutic use are manufactured in mammalian cells that mak ...

EPFL2010

Application of the denaturing gradient gel electrophoresis (DGGE) technique as an efficient diagnostic tool for ciliate communities in soil

Enrique Lara, Alexandre Jousset

Ciliates (or Ciliophora) are ubiquitous organisms which can be widely used as bioindicators in ecosystems exposed to anthropogenic and industrial influences. The evaluation of the environmental impact on soil ciliate communities with methods relying on mor ...

2010