Publications related to Fluorescein

DNA-Liposome Hybrid Carriers for Triggered Cargo Release

The design of simple and versatile synthetic routes to accomplish triggered-release properties in carriers is of particular interest for drug delivery purposes. In this context, the programmability and adaptability of DNA nanoarchitectures in combination w ...

AMER CHEMICAL SOC2022

A 512 x 512 SPAD Image Sensor With Integrated Gating for Widefield FLIM

Edoardo Charbon, Claudio Bruschini, Ivan Michel Antolovic, Arin Can Ülkü

In this paper, we report on SwissSPAD2, an image sensor with 512 x 512 photon-counting pixels, each comprising a single-photon avalanche diode (SPAD), a 1-b memory, and a gating mechanism capable of turning the SPAD ON and OFF, with a skew of 250 and 344 p ...

IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC2019

Cellular Uptake and Intracellular Trafficking of Poly(N-(2-Hydroxypropyl) Methacrylamide)

Anders Meibom, Harm-Anton Klok, Graham Knott, Stéphane Laurent Escrig, Olivier Burri, Arne Seitz, Claudia Battistella, Romain Guiet

Cellular uptake and intracellular trafficking of polymer conjugates or polymer nanoparticles is typically monitored using fluorescence-based techniques such as confocal microscopy. While these methods have provided a wealth of insight into the internalizat ...

American Chemical Society2019

In vivo pulse labeling of isochronic cohorts of cells in the central nervous system using FlashTag

Subashika Govindan

The tracing of neuronal cell lineages is critical to our understanding of cellular diversity in the CNS. This protocol describes a fluorescence birth-dating technique to label, track and isolate isochronic cohorts of newborn cells in the CNS in vivo in mou ...

NATURE PUBLISHING GROUP2018

Preclinical Evaluation of Photoacoustic Imaging as a Novel Noninvasive Approach to Detect an Orthopaedic Implant Infection

Riccardo Sinisi, Pavlo Khodakivskyi, Ghyslain Martial Alain Budin

Introduction: Diagnosing-prosthetic joint infection (PJI) poses significant challenges, and current modalities are fraught with low sensitivity and/or potential morbidity. Photoacoustic imaging (PAI) is a novel ultrasound-based modality with potential for ...

Lippincott Williams & Wilkins2017

Bulk velocity measurements by video analysis of dye tracer in a macro-rough channel

Anton Schleiss, Mário Jorge Rodrigues Pereira Da Franca, Tamara Ghilardi

Steep mountain rivers have hydraulic and morphodynamic characteristics that hinder velocity measurements. The high spatial variability of hydraulic parameters, such as water depth (WD), river width and flow velocity, makes the choice of a representative cr ...

Institute of Physics2014

Reduced Dyes Enhance Single-Molecule Localization Density for Live Superresolution Imaging

Suliana Manley, Luc Reymond, Aleksandr Benke, Grazvydas Lukinavicius, Lina Carlini

Cell-permeable rhodamine dyes are reductively quenched by NaBH4 into a non-fluorescent leuco-rhodamine form. Quenching is reversible, and their fluorescence is recovered when the dyes are oxidized. In living cells, oxidation occurs spontaneously, and can r ...

Wiley-V C H Verlag Gmbh2014

Time-resolved imaging system for fluorescence-guided surgery with lifetime imaging capability

Edoardo Charbon, Claudio Bruschini, Douglas Hanahan, François Eric Powolny, Harald Arjan Robert Homulle, Krisztian Homicsko, Riccardo Sinisi

We present a single-photon camera for fluorescence imaging, with a time resolution better than 100ps, capable of providing both intensity and lifetime images. the camera was fabricated in standard CMOS technology. With this FluoCam we show the possibility ...

Spie-Int Soc Optical Engineering2014

Photobleaching Kinetics and Time-Integrated Emission of Fluorescent Probes in Cellular Membranes

Daniel Sage

Since the pioneering work of Hirschfeld, it is known that time-integrated emission (TiEm) of a fluorophore is independent of fluorescence quantum yield and illumination intensity. Practical implementation of this important result for determining exact prob ...

Mdpi Ag2014

A 2D/3D image analysis system to track fluorescently labeled structures in rod-shaped cells: application to measure spindle pole asymmetry during mitosis

Michaël Unser, Daniel Sage, Viesturs Simanis, Daniel Andreas Schmitter, Anastasia Chasapi, Ioannis Xenarios

Background: The yeast Schizosaccharomyces pombe is frequently used as a model for studying the cell cycle. The cells are rod-shaped and divide by medial fission. The process of cell division, or cytokinesis, is controlled by a network of signaling proteins ...

Biomed Central Ltd2013

Bioluminescent sensor proteins for point-of-care therapeutic drug monitoring

Rudolf Griss

To ensure efficacy but avoid toxicity, many drugs require monitoring of their concentrations in the patient’s blood during therapy. Currently, therapeutic drug monitoring (TDM) is done in diagnostic laboratories that need trained personnel and expensive in ...

EPFL2013

Controlled synthesis of fluorescent silica nanoparticles inside microfluidic droplets

Martinus Gijs, Virendra Kumar Parashar, Josias Basil Wacker, Ioannis Lignos

We study the droplet-based synthesis of fluorescent silica nanoparticles (50–350 nm size) in a microfluidic chip. Fluorescein-isothiocyanate (FITC) dye is first chemically linked to aminopropyl triethoxysilane (APTES) in ethanol and this reaction product i ...

Royal Society of Chemistry2012

On the Applicability of Oil and Dye Technique During the Design Phase and Operation of Experimental Rigs

Alexandros Terzis

In this paper, an oil and dye flow visualization technique applied in two different cases of experimental testing is described, namely a single fan in crossflow and the wake of a compressor cascade. In both cases, the mixture of paint was prepared using a ...

2011

Vertical arrays of nanofluidic channels fabricated without nanolithography

Klaus Kern, Floriano Traversi, Kannan Balasubramanian

Vertical arrays of sealed nanofluidic channels, in which both cross-sectional dimensions are controllable down to 10 nm, were fabricated by selective side etching of a SiGe heterostructure comprised of layers of alternating Ge fractions. Capillary filling ...

2009

Printing color images visible under uv light on security documents and valuable articles

Roger Hersch, Sylvain Chosson, Philipp Donzé

In the present invention, we propose a method of creating fluorescent color images visible under UV light. It relies on the new colorants that can be achieved by superposing ink dots, possibly at a reduced size, in order to avoid quenching effects. It also ...

2008

Spectral prediction model for color prints on paper with fluorescent additives

Roger Hersch

I propose a model for predicting the total reflectance of color halftones printed on paper incorporating fluorescent brighteners. The total reflectance is modeled as the additive superposition of the relative fluorescent emission and the pure reflectance o ...

2008

Glucose and lactate are equally effective in energizing activity-dependent synaptic vesicle turnover in purified cortical neurons

Pierre Magistretti, Jean-Yves Chatton

This study examines the role of glucose and lactate as energy substrates to sustain synaptic vesicle cycling. Synaptic vesicle turnover was assessed in a quantitative manner by fluorescence microscopy in primary cultures of mouse cortical neurons. An elect ...

Elsevier2006

Modelling the effect of soil water content and sorption on dye-tracer fluorescence

Fluorescent dye tracers are used to show concentration maps of tracer fronts, plumes and pathways on soil profiles and in monolith cross sections. The physical process of fluorescence from the exposed soil surfaces must be understood in order to retrieve a ...

2005

Covalent labeling and chemical induced dimerization of fusion proteins

A new method was developed for the specific and covalent labeling of fusion proteins with small organic probes in vitro and in vivo. The method is based on the construction of fusion proteins of the human O6-alkylguanine-DNA alkyltransferase (hAGT) with a ...

EPFL2005

Electroosmotic Flow in Composite Microchannels and Implications in Microcapillary Electrophoresis Systems

Hubert Girault, Patrik Willi Hoffmann, François Bianchi, Frank Rüdiger Wagner

The electroosmotic flow in laminated excimer laser-ablated microchannels has been studied as a function of the depth of the rectangular channels, and particular emphasis has been given to the difference in the ζ-potentials between the lamination layer and ...

2001