Publications related to Tryptophan

Dynamic partitioning of branched-chain amino acids-derived nitrogen supports renal cancer progression

Vassily Hatzimanikatis, Maria Masid Barcon, Ming Yang

Metabolic reprogramming is critical for tumor initiation and progression. However, the exact impact of specific metabolic changes on cancer progression is poorly understood. Here, we integrate multimodal analyses of primary and metastatic clonally-related ...

Nature Portfolio2022

Impairment of Glycolysis-Derived L-Serine Production in Astrocytes Contributes to Cognitive Deficits in Alzheimer's Disease

Graham Knott

Alteration of brain aerobic glycolysis is often observed early in the course of Alzheimer's disease (AD). Whether and how such metabolic dysregulation contributes to both synaptic plasticity and behavioral deficits in AD is not known. Here, we show that th ...

Cell Press2020

Synthetic Studies toward Amanita Phalloides Toxins

Philipp Georg Werner Seeberger

Amatoxins are ribosomally synthesized and post-translationally modified bicyclic octapeptides biosynthesized by the deadly basidiomycete fungus Amanita phalloides. Amongst this group, alpha-amanitin is the most widely known toxin and is currently under inv ...

EPFL2020

Analyzing glycans cleaved from a biotherapeutic protein using ultrahigh-resolution ion mobility spectrometry together with cryogenic ion spectroscopy

Thomas Rizzo, Priyanka Bansal, Ali H Abikhodr, Vasyl Yatsyna, Natalia Yalovenko

Glycans covalently attached to protein biotherapeutics have a significant impact on their biological activity, clearance, and safety. As a result, glycosylation is categorized as a critical quality attribute that needs an adequate analytical approach to gu ...

2020

Two-photon triggered photorelease of caged compounds from multifunctional harmonic nanoparticles

Sandrine Gerber, Laure Menin, Daniel Ortiz Trujillo, Jérémy Vuilleumier, Raphaël Jovita De Matos, Luigi Bonacina

The design of stimuli-responsive nanocarriers has raised much attention to achieve higher local concentration of therapeutics and mitigate the appearance of drug resistance. The combination of imaging properties and controlled photorelease of active molecu ...

2019

Comparison of the functional properties of trimeric and monomeric CaiT of Escherichia coli

Kamila Krystyna Guérin

Secondary transporters exist as monomers, dimers or higher state oligomers. The significance of the oligomeric state is only partially understood. Here, the significance of the trimeric state of the L-carnitine/gamma-butyrobetaine antiporter CaiT of Escher ...

Nature Research2019

Calcium phosphate formation on titanium-based surfaces: bioactivity evaluated by in vitro methods

Weitian Zhao

The prediction of implant behaviors in vivo by the use of easy-to-perform in vitro methods is of great interest in biomaterials research. In the field of bone implants, it is commonly accepted that a spontaneous formation of a hydroxyapatite (HA) layer on ...

EPFL2018

Role of ACMSD in regulation of NAD+ metabolism and mitochondrial function

Elena Katsyuba

Discovered in the beginning of the 20th century, nicotinamide adenine dinucleotide (NAD+) has evolved from a simple oxidoreductase cofactor to being an essential cosubstrate for a wide range of regulatory proteins that include most notoriously the sirtuin ...

EPFL2017

Multilaser Conformer-Selective Spectroscopy of Cold Biomolecular Ions in the Gas Phase

Aleksandr Pereverzev

Proteins and even small peptides play crucial role in almost any biological. Biological activity and functionality of these building blocks of life are determined not only by their primary structure, but also by their 3D orientation â secondary, tertiary ...

EPFL2017

Scanning tunneling microscopy on large bio-molecular systems on surfaces

Sebastian Koslowski

The ever growing demand for the development of new technologies and materials has led to extensive studies inspired by biological functional complexes. Peptides with their outstanding ability to efficiently self-assemble can express a broad spectrum of int ...

EPFL2017

Mechanistic study of the radical SAM-dependent amine dehydrogenation reactions

Xinjian Ji, Shuguang Yuan, Qixing Zhang, Yue Yin

The radical SAM enzyme NosL catalyzes the conversion of L-Trp to 3-methyl-2-indolic acid, and this reaction is initiated by the 5'-deoxyadenosyl (dAdo) radical-mediated hydrogen abstraction from the L-Trp amino group. We demonstrate here that when D-Trp wa ...

Royal Society of Chemistry2016

Host genetic predictors of the kynurenine pathway of tryptophan catabolism among treated HIV-infected Ugandans

Yunfei Huang

Objective: Plasma kynurenine/tryptophan ratio, a biomarker of indoleamine 2,3-dioxygenase-1 (IDO) activity, is a strong independent predictor of mortality in HIV-infected Ugandans initiating antiretroviral therapy (ART) and may play a key role in HIV patho ...

Lippincott Williams & Wilkins2016

The Crystal Structures of Apo and cAMP-Bound GlxR from Corynebacterium glutamicum Reveal Structural and Dynamic Changes upon cAMP Binding in CRP/FNR Family Transcription Factors

Stewart Cole, Florence Pojer

The cyclic AMP-dependent transcriptional regulator GlxR from Corynebacterium glutamicum is a member of the super-family of CRP/FNR (cyclic AMP receptor protein/fumarate and nitrate reduction regulator) transcriptional regulators that play central roles in ...

Public Library of Science2014

Ultrafast Tryptophan-to-Heme Electron Transfer in Myoglobins Revealed by UV 2D Spectroscopy

Majed Chergui, Frank van Mourik, Cristina Consani, Gerald Auböck

Tryptophan is commonly used to study protein structure and dynamics, such as protein folding, as a donor in fluorescence resonant energy transfer (FRET) studies. By using ultra-broadband ultrafast two-dimensional (2D) spectroscopy in the ultraviolet (UV) a ...

American Association for the Advancement of Science2013

Coherent manipulation of free amino acids fluorescence

Christoph Peter Hauri

Coherent manipulation of molecular wavepackets in biomolecules might contribute to the quest towards label-free cellular imaging and protein identification. We report the use of optimally tailored UV laser pulses in pump-probe depletion experiments that se ...

2012

Photophysics and Photochemistry from First Principles : Applications to Problems in Materials Science and Biology

Matteo Guglielmi

Quantum chemical methods are important tools for the predictions of electronic structure and energetics of molecules providing the interpretation of spectroscopic experiments and reaction mechanisms. In this thesis, density functional theory (DFT) and wave ...

EPFL2010

Functional electric field changes in photoactivated proteins revealed by ultrafast Stark spectroscopy of the Trp residues

Majed Chergui, Frank van Mourik, Andrea Cannizzo, Erwin Portuondo Campa, Stefan Haacke

Ultrafast transient absorption spectroscopy of wild-type bacteriorhodopsin (WT bR) and 2 tryptophan mutants (W86F and W182F) is performed with visible light excitation (pump) and UV probe. The aim is to investigate the photoinduced change in the charge dis ...

2009

Electronic and vibrational spectroscopy of cold protonated amino acids in the gas phase

Sébastien Mercier

In this thesis report, we describe a novel home-built instrument designed to study the spectroscopy of biomolecular ions in the gas phase and at low temperature, and we present the first experimental results obtained on protonated aromatic amino acids. The ...

EPFL2008

Microsolvation effects on the excited state dynamics of protonated tryptophan

Ursula Röthlisberger, Thomas Rizzo, Ivano Tavernelli, Oleg Boyarkine, Sébastien Mercier, Michele Cascella, Matteo Guglielmi, Anthi Kamariotou

To better understand the complex photophysics of the amino acid tryptophan, which is widely used as a probe of protein structure and dynamics, we have measured electronic spectra of protonated, gas-phase tryptophan solvated with a controlled number of wate ...

2006

Insights into excited-state and isomerization dynamics of bacteriorhodopsin from ultrafast transient UV absorption

Majed Chergui, Frank van Mourik, Selma Schenkl

A visible-pump/UV-probe transient absorption is used to characterize the ultrafast dynamics of bacteriorhodopsin with 80-fs time resoln. We identify three spectral components in the 265- to 310-nm region, related to the all-trans retinal, tryptophan (Trp)- ...

National Academy of Sciences2006