Publications related to Pre-replication complex

Resolving bacterial cell biology: from replisome dynamics to cell wall synthesis

Bacteria are ubiquitous single cellular organisms. Compared to eukaryotic cells, bacteria have two unique characteristics: the membrane-less nucleoid and the cell wall built of peptidoglycan (PG). In most bacteria, a single circular chromosome is compacted ...

EPFL2024

Robust designation of meiotic crossover sites by CDK-2 through phosphorylation of the MutS gamma complex

Alexander Woglar

Crossover formation is essential for proper segregation of homologous chromosomes during meiosis. Here, we show that Caenorhabditis elegans cyclin-dependent kinase 2 (CDK-2) partners with cyclin-like protein COSA-1 to promote crossover formation by promoti ...

National Academy of Sciences2022

Identification and characterization of proteins that protect human telomeres from fragility

Anna Christina Näger

Difficulties to replicate telomeres - the ends of our chromosomes - can cause telomere shortening and genome instability. These difficulties are due to the repetitive DNA sequence and distinct structures at telomeres that challenge the semi-conservative DN ...

EPFL2022

Coordination between cell growth, division and chromosome replication cycles in mycobacteria

Gaëlle Madeleine Vuaridel

Propagation of microorganisms is based on three fundamental processes: cell growth, DNA replication, and cell division. Although important for antibacterial drug development, these processes are poorly understood in Actinobacteria, a medically important ph ...

EPFL2021

PaRiS: Causally Consistent Transactions with Non-blocking Reads and Partial Replication

Willy Zwaenepoel, Diego Didona, Kristina Spirovska

Geo-replicated data platforms are at the backbone of several large-scale online services. Transactional Causal Consistency (TCC) is an attractive consistency level for building such platforms. TCC avoids many anomalies of eventual consistency, eschews the ...

2019

PaRiS: Causally Consistent Transactions with Non-blocking Reads and Partial Replication

Willy Zwaenepoel, Diego Didona, Kristina Spirovska

Geo-replicated data platforms are the backbone of several large-scale online services. Transactional Causal Consistency (TCC) is an attractive consistency level for building such platforms. TCC avoids many anomalies of eventual consistency, eschews the syn ...

IEEE COMPUTER SOC2019

Analysis of the S. pombe Meiotic Proteome Reveals a Switch from Anabolic to Catabolic Processes and Extensive Post-transcriptional Regulation

Viesturs Simanis, Andrea Krapp, Romain Hamelin, Diego Chiappe, Florence Armand, Lucien Fabrice Krapp, Marc Moniatte, Elena Cano

Meiotic progression in S. pombe is regulated by stage-specific gene expression and translation, changes in RNA stability, expression of anti-sense transcripts, and targeted proteolysis of regulatory proteins. We have used SILAC labeling to examine the rela ...

2019

Premature activation of Cdk1 leads to mitotic events in S phase and embryonic lethality

João Pedro de Matos Rodrigues, Patricia Renck Nunes

Cell cycle regulation, especially faithful DNA replication and mitosis, are crucial to maintain genome stability. Cyclin-dependent kinase (CDK)/cyclin complexes drive most processes in cellular proliferation. In response to DNA damage, cell cycle surveilla ...

Springer2019

An essential thioredoxin is involved in the control of the cell cycle in the bacterium Caulobacter crescentus

Camille Véronique Bernadette Goemans

Thioredoxins (Trxs) are antioxidant proteins that are conserved among all species. These proteins have been extensively studied and perform reducing reactions on a broad range of substrates. Here, we identified Caulobacter crescentus Trx1 (CCNA_03653; CcTr ...

2018

Transcription-induced supercoiling explains formation of self-interacting chromatin domains in S-pombe

Yannis Burnier, Julien Dorier, Fabrizio Benedetti, Andrzej Stasiak

The question of how self-interacting chromatin domains in interphase chromosomes are structured and generated dominates current discussions on eukaryotic chromosomes. Numerical simulations using standard polymer models have been helpful in testing the vali ...

Oxford University Press (OUP)2017

Mycobacterium tuberculosis Resists Stress by Regulating PE19 Expression

John McKinney, Anna De Graff Tischler

Mycobacterium tuberculosis requires the phosphate-sensing signal transduction system Pst/SenX3-RegX3 to resist host immune responses. A Delta pstA1 mutant lacking a Pst phosphate uptake system component is hypersensitive to diverse stress conditions in vit ...

American Society for Microbiology2016

Aurora A kinase regulates proper spindle positioning in C-elegans and in human cells

Pierre Gönczy, Coralie Busso, Sachin Kotak

Accurate spindle positioning is essential for error-free cell division. The one-cell Caenorhabditis elegans embryo has proven instrumental for dissecting mechanisms governing spindle positioning. Despite important progress, how the cortical forces that act ...

2016

Chromosome Organization and Replisome Dynamics in Mycobacterium smegmatis

John McKinney, Isabella Santi

Subcellular organization of the bacterial nucleoid and spatiotemporal dynamics of DNA replication and segregation have been studied intensively, but the functional link between these processes remains poorly understood. Here we use quantitative time-lapse ...

American Society for Microbiology2015

An Extended, Boolean Model of the Septation Initiation Network in S-pombe Provides Insights into Its Regulation

Viesturs Simanis, Andrea Krapp, Elena Cano, Anastasia Chasapi, Ioannis Xenarios

Cytokinesis in fission yeast is controlled by the Septation Initiation Network (SIN), a protein kinase signaling network using the spindle pole body as scaffold. In order to describe the qualitative behavior of the system and predict unknown mutant behavio ...

Public Library of Science2015

Functional Perturbation of the Gab2 Multiprotein Complex by High-affinity SH2-binding Monobodies in Chronic Myelogenous Leukemia

Emel Basak Gencer Akçok

Chronic myeloid leukemia is characterized by a reciprocal chromosomal translocation between chromosome 9 and 22, resulting in the expression of the Bcr-Abl oncoprotein. Despite the great improvement in patient survival using tyrosine kinase inhibitors (TKI ...

EPFL2015

Generating photocurrent by nanocomposites based on photosynthetic reaction centre protein

László Forró, Endre Horvath, Klara Hernadi, Emil Nyerki

An optoelectronic device, which converts light energy to electric potential, was designed and fabricated by using photosynthetic reaction centre (RC) proteins of purple bacterium Rhodobacter sphaeroides R-26, based on the structure and function of the dye ...

Wiley-V C H Verlag Gmbh2015

Long-Term Single Cell Analysis of S. pombe on a Microfluidic Microchemostat Array

Sebastian Maerkl, Jean-Bernard Nobs

Although Schyzosaccharomyces pombe is one of the principal model organisms for studying the cell cycle, surprisingly few methods have characterized S. pombe growth on the single cell level, and no methods exist capable of analyzing thousands of cells and t ...

Public Library of Science2014

Carbon nanotubes quench singlet oxygen generated by photosynthetic reaction centers

László Forró, Arnaud Magrez, Endre Horvath, Klara Hernadi, Krisztina Nagy

Photosensitizers may convert molecular oxygen into reactive oxygen species (ROS) including, e.g., singlet oxygen (O-1(2)), superoxide anion (O-2(-center dot)), and hydroxyl radicals ((OH)-O-center dot), chemicals with extremely high cyto- and potential gen ...

Wiley-V C H Verlag Gmbh2013

Hyperbranched Polylysine: A Versatile, Biodegradable Transfection Agent for the Production of Recombinant Proteins by Transient Gene Expression and the Transfection of Primary Cells

Harm-Anton Klok, Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Zuzana Kadlecova, Yashas Rajendra

The feasibility of a new transfection agent, HBPL, for the production of recombinant IgG antibody via TGE as well as for the transfection of primary cells is studied. Under the conditions investigated, transfection of CHO-DG44 cells using HBPL results in I ...

2012

Charge stabilization by reaction center protein immobilized to carbon nanotubes functionalized by amine groups and poly(3-thiophene acetic acid) conducting polymer

László Forró, Arnaud Magrez, Endre Horvath, Klara Hernadi

A large number of studies have indicated recently that photosynthetic reaction center proteins (RC) bind successfully to nanostructures and their functional activity is largely retained. The major goal of current research is to find the most efficient syst ...

Wiley-V C H Verlag Gmbh2012