Publications related to Bacillus subtilis

Probing the growth and mechanical properties of Bacillus subtilis biofilms through genetic mutation strategies

Bacterial communities form biofilms on various surfaces by synthesizing a cohesive and protective extracellular matrix, and these biofilms protect microorganisms against harsh environmental conditions. Bacillus subtilis is a widely used experimental specie ...

KEAI PUBLISHING LTD2022

Analysis of a bac operon-silenced strain suggests pleiotropic effects of bacilysin in Bacillus subtilis

Mustafa Demir

Bacilysin, as the simplest peptide antibiotic made up of only L-alanine and L-anticapsin, is produced and excreted by Bacillus subtilis under the control of quorum sensing. We analyzed bacilysin-nonproducing strain OGU1 which was obtained by bacA-targeted ...

MICROBIOLOGICAL SOCIETY KOREA2020

Mechanistic insights into bacterial metabolic reprogramming from omics-integrated genome-scale models

Noushin Hadadi, Vikash Kumar Pandey, Anush Chiappino-Pepe, Merten Morales, Florence Mehl

Understanding the adaptive responses of individual bacterial strains is crucial for microbiome engineering approaches that introduce new functionalities into complex microbiomes, such as xenobiotic compound metabolism for soil bioremediation. Adaptation re ...

NATURE PUBLISHING GROUP2020

Single-cell studies of Mycobacterium smegmatis cell cycle using time-lapse fluorescence microscopy

Joëlle Xiao Yuan Ven

Cell division is one of the most primordial cellular processes and is essential for life propagation. It is composed of crucial events, namely genome duplication, chromosomes segregation and membrane separation. Failure of these processes will have dramati ...

EPFL2019

Microbiological contamination of cosmetic products - observations from Europe, 2005-2018

Florentino Luciano Caetano Dos Santos

Background Although cosmetic products are not expected to be fully aseptic, their potential microbiological contamination might be dangerous, especially for immunocompromised patients. The extent of such contamination of cosmetics in Europe is poorly studi ...

2019

Mechanisms of DNA Uptake by Naturally Competent Bacteria

Melanie Blokesch

Transformation is a widespread mechanism of horizontal gene transfer in bacteria. DNA uptake to the periplasmic compartment requires a DNA-uptake pilus and the DNA-binding protein ComEA. In the gram-negative bacteria, DNA is first pulled toward the outer m ...

2019

Vibrio cholerae filamentation promotes chitin surface attachment at the expense of competition in biofilms

Alexandre Louis André Persat

Collective behavior in spatially structured groups, or biofilms, is the norm among microbes in their natural environments. Though biofilm formation has been studied for decades, tracing the mechanistic and ecological links between individual cell morpholog ...

National Academy of Sciences2019

Steel Wire Mesh as a Thermally Resistant SERS Substrate

Tomasz Radoslaw Szymborski

In this paper, we present novel type of Surface-enhanced Raman spectroscopy (SERS) platform, based on stainless steel wire mesh (SSWM) covered with thin silver layer. The stainless steel wire mesh, typically used in chemical engineering industry, is a chea ...

MDPI2018

Bacterial traps and advances in nanobiotechnology for atomic force microscopy

Oliver Peric

The atomic force microscope (AFM) allows the analysis of living microorganisms in physiological conditions on the nanometer scale. The observation of bacteria in physiological aqueous medium necessitates a robust immobilization of the bacterium to the surf ...

EPFL2018

Photothermal Off-Resonance Tapping for Rapid and Gentle Atomic Force Imaging of Live Cells

Georg Fantner, John McKinney, Adrian Pascal Nievergelt, Haig Alexander Eskandarian, Charlène Cécile Geneviève Brillard

Imaging living cells by atomic force microscopy (AFM) promises not only high-resolution topographical data, but additionally, mechanical contrast, both of which are not obtainable with other microscopy techniques. Such imaging is however challenging, as ce ...

2018

More Medicines for Tuberculosis: Fuelling Drug Discovery against Mycobacterium tuberculosis

Shi-Yan Caroline Foo

Tuberculosis (TB), whose etiological agent is Mycobacterium tuberculosis (M. tuberculosis), has plagued humanity since antiquity. Even with chemotherapy available today, TB is the leading cause of death due to an infectious disease. Modern day factors, suc ...

EPFL2018

Genotyping Of Bacillus Spp. Isolate Collection From Natural Samples

The aim of this study was genotyping and identification of collection of 164 Bacillus spp. isolates, from samples of soil, manure, and straw gathered from across Serbia, using Pulse field gel electrophoresis (PFGE) combined with sequencing of tuf gene, one ...

2017

Rae1/YacP, a new endoribonuclease involved in ribosome-dependent mRNA decay in Bacillus subtilis

Jérémie Piton

The PIN domain plays a central role in cellular RNA biology and is involved in processes as diverse as rRNA maturation, mRNA decay and telomerase function. Here, we solve the crystal structure of the Rae1 (YacP) protein of Bacillus subtilis, a founding mem ...

Wiley2017

Microfluidic bacterial traps for simultaneous fluorescence and atomic force microscopy

Oliver Peric, Georg Fantner, Jonathan David Adams, Mélanie Thérèse Marie Hannebelle

The atomic force microscope has become an established research tool for imaging microorganisms with unprecedented resolution. However, its use in microbiology has been limited by the difficulty of proper bacterial immobilization. Here, we have developed a ...

2017

Synthesis, structural elucidation and biological activities of organotin(IV) derivatives of 4-(2-thienyl)butyric acid

Paul Joseph Dyson

A series of tri- and diorganotin(IV) derivatives of 4-(2-thienyl)butyric acid have been synthesized by the reaction of ligand acid with tri- and diorganotin salts in 1:1 and 2:1 molar ratios, respectively. The synthesized compounds have been confirmed by C ...

Springer2017

Circulation of a Quorum-Sensing-Impaired Variant of Vibrio cholerae Strain C6706 Masks Important Phenotypes

Melanie Blokesch, Sandrine Stutzmann

Vibrio cholerae, the causative agent of cholera, is a model organism for studying virulence regulation, biofilm formation, horizontal gene transfer, and the cell-to-cell communication known as quorum sensing (QS). As in any research field, discrepancies be ...

Amer Soc Microbiology2016

A benzamide-dependent ftsZ mutant reveals residues crucial for Z-ring assembly

David William Adams

In almost all bacteria, cell division is co-ordinated by the essential tubulin homologue FtsZ and represents an attractive but as yet unexploited target for new antibiotics. The benzamides, e.g. PC190723, are potent FtsZ inhibitors that have the potential ...

Wiley-Blackwell2016

Functional genomics of corrinoid starvation in the organohalide-respiring bacterium Dehalobacter restrictus strain PER-K23

Christof Holliger, Julien Maillard, Yue Lu, Aamani Rupakula Boyanapalli, Hauke Smidt

De novo comnoid biosynthesis represents one of the most complicated metabolic pathways in nature. Organohalide-respiring bacteria (OHRB) have developed different strategies to deal with their need of corrinoid, as it is an essential cofactor of reductive d ...

Frontiers Media SA2015

Investigation of sporulation in the Desulfotomaculum genus: a genomic comparison with the genera Bacillus and Clostridium

Rizlan Bernier-Latmani, Elena Cristina Dalla Vecchia

The genus Desulfotomaculum, belonging to the Firmicutes, comprises strictly anaerobic and endospore-forming bacteria capable of dissimilatory sulfate reduction. These microorganisms are metabolically versatile and are widely distributed in the environment. ...

Blackwell Publishing2014

The Crystal Structures of Apo and cAMP-Bound GlxR from Corynebacterium glutamicum Reveal Structural and Dynamic Changes upon cAMP Binding in CRP/FNR Family Transcription Factors

Stewart Cole, Florence Pojer

The cyclic AMP-dependent transcriptional regulator GlxR from Corynebacterium glutamicum is a member of the super-family of CRP/FNR (cyclic AMP receptor protein/fumarate and nitrate reduction regulator) transcriptional regulators that play central roles in ...

Public Library of Science2014