Lectures in course CH-313: Chemical biology

Chemical Biology meets Drug Discovery

Explores Chemical Biology, Drug Discovery, enzyme kinetics, and inhibition methods, including competitive and non-competitive inhibition.

Interrogating Drugs' Mode of Action

Delves into enzyme inhibition, reversible and irreversible binding, and covalent drugs, exploring drug modes of action and their impact on drug efficacy.

Metabolic Pathways Tracking: Isotope Tracing Profiling

Explores tracking metabolic pathways through isotope tracing for quantitative metabolomics profiling.

Electron Transport Chain: Proton Pumps and Coenzyme Q

Discusses the electron transport chain in mitochondria and the role of Coenzyme Q.

Protein Profiling: Mass Spectrometry and Target-ID Tools

Explores protein profiling using mass spectrometry, target enrichment techniques, and quantitative proteomics.

Activity-based Protein Profiling: Druggable Sites Identification

Explores Activity-based Protein Profiling for druggable site identification and strategies to minimize off-target reactivity of covalent inhibitors.

Electron Transport Chain: Underlying Chemistry and Biology

Explores the Electron Transport Chain and Oxidative Phosphorylation processes, focusing on the chemistry and biology behind energy generation.

ATP Synthase: Energy Production and Cellular Respiration

Explores ATP synthase, cellular energy production, and monitoring methods.

Chemical Biology Tools: Genome Interrogation

Explores Chemical Biology tools for genome interrogation, covering central dogma, cell cycle phases, nucleosides, and chemical biology toolsets.

Cellular Energy Production: ETC-OXPHOS & ATP Synthesis

Explores ETC-OXPHOS, ATP synthesis, ECAR, OCR, and metabolic modulators.

Chemical Biology Tools: Transcriptome Interrogation

Covers chemical biology tools for RNA study and explores the applications of GFP in biosciences.

Cell Energy Monitoring Tools

Explores tools for monitoring cell energy status and a case study on breast tumor aggressiveness.

Tools for Translatome Analysis

Explores tools for studying the translatome, including mRNA translation and genetic code expansion.

Genetic Code Expansion: Tools and Applications

Explores Genetic Code Expansion technology for incorporating unnatural amino acids in mRNA translation.

Targeting Glycolysis and Cancer Signaling

Explores targeting glycolysis and cancer signaling switches, enzyme inhibition mechanisms, and the role of ATP as the body's energy currency.

Probing Translation Dynamics: Ribosome Profiling & ER Engagement

Explores translation dynamics, ribosome profiling, ER engagement, and protein trafficking in cells.