CH-319: Experimental biochemistry and biophysics

Lectures in this course (10)

Covers transforming plasmid DNA into bacteria and isolating DNA from bacterial colonies.

Covers genetic engineering, FRET sensors, PCR cloning, and DNA constructs for practical applications.

Covers scientific writing, formatting, citations, and the behavior of condensed chromatin.

Delves into circular permutation in protein engineering, focusing on optimizing proteins by introducing new termini without changing the structure.

Explores the use of ApE for planning and analyzing cloning projects.

Covers the purification of proteins using IMAC chromatography and analysis methods.

Covers the purification of expressed proteins using methods like transformation, lysis, IMAC, and protein analysis.

Explores protein expression systems, vector design, and solubility enhancement in E. coli and other common systems.

Introduces students to genetic engineering, FRET sensors, protein characterization, and lab safety in biochemistry and biophysics.

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