Explores the evolution of DNA sequencing techniques, from shotgun sequencing to real-time PCR, emphasizing the importance of direct RNA sequencing and the challenges of repetitive DNA sequences.
Introduces fundamental concepts of fluorescence in microscopy, covering terms like excitation and emission spectra, Stokes shift, quantum efficiency, brightness, and photobleaching.
Covers the introduction to fluorescence in microscopy, labeling techniques, specificity of markers, multi-color imaging, and factors affecting fluorescence properties.
