Covers resolution enhancement in optical microscopy, confocal imaging, and fluorescent labelling, exploring the history and techniques of scanning probe microscopy.
Introduces fundamental concepts of fluorescence in microscopy, covering terms like excitation and emission spectra, Stokes shift, quantum efficiency, brightness, and photobleaching.
Covers the introduction to fluorescence in microscopy, labeling techniques, specificity of markers, multi-color imaging, and factors affecting fluorescence properties.
Delves into advancements in optical imaging, overcoming diffraction limits, and imaging through complex media, with a focus on biological applications.