Lectures related to Protein Expression Systems

Protein Synthesis: Recombinant Expression

Explores protein synthesis, genetic code, transcription, translation, and recombinant protein expression using vectors and plasmids.

Protein Expression & Purification

Explores protein expression, purification, and gel filtration for isolating protein complexes.

DNA: PCR and Sequencing Techniques

Explores DNA engineering through PCR, Sanger sequencing, the Human Genome Project, recombinant DNA, bacterial DNA manipulation, and CRISPR/Cas9.

Protein Chromatography: Purification Techniques

Explores protein chromatography methods, including affinity purification and tag removal, for efficient protein purification processes.

Experimental Biochemistry and Biophysics: Genetic Engineering and FRET Sensors

Introduces students to genetic engineering, FRET sensors, protein characterization, and lab safety in biochemistry and biophysics.

Genetics: History and Key Concepts

Covers the history of genetics, key concepts, DNA structure, gene expression, and genetic engineering.

Protein Production: Engineering Bacteria for Protein Synthesis

Covers the engineering of bacteria for efficient protein production and purification techniques using affinity tags.

Types of Viral Vectors

Explores the properties of commonly used viral vectors for gene therapy.

Protein Expression and Purification

Covers the process of expressing and purifying proteins, including the importance of studying protein function, folding, interactions, sequence, and structure.

Gene Expression: Introduction and Data Analysis

Covers gene expression data quantification, genome analysis, and large-scale studies.

Production of Viral Vectors

Explores the production of viral vectors for gene therapy applications, emphasizing AAV vectors and the process of large-scale production and purification.

Protein Life Cycle

Covers gene expression, protein life cycle, and genetic code in neuroscience.

Bulk Transcriptome Analysis: RNA-seq Workflow and Gene Expression Analysis

Explains transcriptomics, RNA-seq workflow, and gene expression analysis for disease biomarkers.

Rules of Development

Delves into the rules of development, emphasizing the role of signals and morphogens.

Genetic Circuits: Synthetic Biology

Explores genetic circuits design in synthetic biology, from regulatory mechanisms to plasmid construction.

Gene Regulatory Networks

Explores how gene regulatory networks encode changes in cell development and the importance of interactions between different regulatory networks.

Alternative Splicing: From RNA to Protein

Explores alternative splicing in gene expression to create diverse proteins.

Regulation Mechanisms Overview

Covers phosphorylation, ubiquitinylation, methylation, and more in biological regulation.

Gene Therapy: Neurological Diseases

Explores gene therapy for neurological diseases, including monogenic and multifactorial disorders, and the use of Cas9 and repair mechanisms.

Preclinical and Clinical Trials in SMA Gene Therapy

Explores preclinical and clinical trials in SMA gene therapy, including efficiency in patients, trial results, adverse effects, cellular targeting, and future perspectives.