Lectures related to Experimental Biochemistry and Biophysics: Genetic Engineering and FRET Sensors

Protein Production: Engineering Bacteria for Protein Synthesis

Covers the engineering of bacteria for efficient protein production and purification techniques using affinity tags.

Protein Synthesis: Recombinant Expression

Explores protein synthesis, genetic code, transcription, translation, and recombinant protein expression using vectors and plasmids.

Immune System Defense

Covers the immune system defense mechanisms, restriction enzymes, genetic engineering, and the CRISPR-Cas system applications.

Genetics: History and Key Concepts

Covers the history of genetics, key concepts, DNA structure, gene expression, and genetic engineering.

Protein Expression Systems

Explores protein expression systems, vector design, and solubility enhancement in E. coli and other common systems.

DNA: PCR and Sequencing Techniques

Explores DNA engineering through PCR, Sanger sequencing, the Human Genome Project, recombinant DNA, bacterial DNA manipulation, and CRISPR/Cas9.

Protein Chromatography: Purification Techniques

Explores protein chromatography methods, including affinity purification and tag removal, for efficient protein purification processes.

Protein Expression & Purification

Explores protein expression, purification, and gel filtration for isolating protein complexes.

Protein Labeling Techniques

Covers protein labeling techniques using self-labeling parts like FIASH and SNAP.

Protein Function Regulation: Degradation Mechanisms

Covers the mechanisms of protein degradation, including the roles of the proteasome and autophagy in regulating protein function.

Protein Life Cycle

Covers gene expression, protein life cycle, and genetic code in neuroscience.

Protein Expression and Purification

Covers the process of expressing and purifying proteins, including the importance of studying protein function, folding, interactions, sequence, and structure.

Obesity and Genetic Mutations: Impacts on Weight Regulation

Explores genetic mutations affecting weight regulation and gene expression in mice.

Protein Circuit Design

Explores the design of protein circuits for manipulating cellular functions and highlights the importance of engineered cells in synthetic biology.

Proteomics: Post-Translational Modifications and Interactions

Explores proteomics for post-translational modifications and protein interactions.

Gene Editing and DNA Origami: Innovations in Biotechnology

Discusses recombinant DNA technology, CRISPR/Cas9 gene editing, and DNA origami applications in biotechnology.

DNA and Its Engineering: Fundamentals and Applications

Provides an overview of DNA structure, replication, transcription, and its engineering applications in molecular biology.

Gene Therapy: Neurological Diseases

Explores gene therapy for neurological diseases, including monogenic and multifactorial disorders, and the use of Cas9 and repair mechanisms.

Bulk Transcriptome Analysis: RNA-seq Workflow and Gene Expression Analysis

Explains transcriptomics, RNA-seq workflow, and gene expression analysis for disease biomarkers.

Genomics: Gene Regulatory Networks

Explores gene regulatory networks, protein-DNA interactions, and regulatory elements in genomics.