Introduces fundamental concepts of fluorescence in microscopy, covering terms like excitation and emission spectra, Stokes shift, quantum efficiency, brightness, and photobleaching.
Explores the interaction of light with molecules, including exciton formation, absorption and emission spectra, and selection rules for electronic transitions.
Explores light dosimetry in tissues for photomedicine applications, covering mechanisms of light therapy, laser classification, and the influence of laser parameters on treatment outcomes.
Explores fluorescence lifetime in photomedicine, covering health effects of artificial light, fluorescence guided surgery, and applications of optical spectroscopy.
Covers the introduction to fluorescence in microscopy, labeling techniques, specificity of markers, multi-color imaging, and factors affecting fluorescence properties.
