Publications by Tristan Colomb

Versatile spectral modulation of a broadband source for digital holographic microscopy

Tristan Colomb, Gaël Nardin, Christophe Moser

We demonstrate the potential of spatial light modulators for the spectral control of a broadband source in digital holographic microscopy. Used in a ‘pulse-shaping’ geometry, the spatial light modulator provides a versatile control over the bandwidth and w ...

Optical Society of America2016

Digital holographic reflectometry

Tristan Colomb, Christian Depeursinge, Jonas Kühn, Nicolas Pavillon, Ahmet Ata Akatay

Digital holographic microscopy (DHM) is an interferometric technique that allows real-time imaging of the entire complex optical wavefront (amplitude and phase) reflected by or transmitted through a sample. To our knowledge, only the quantitative phase is ...

2010

Extended depth-of-focus by digital holographic microscopy

Tristan Colomb, Etienne Cuche, Christian Depeursinge, Jonas Kühn, Nicolas Pavillon

A recurrent problem in microscopy is the finite depth-of-focus linked to the NA of microscope objectives. Digital holographic microscopy (DHM) has the unique feature of being able to numerically change the focus from a single hologram without the need of m ...

Optical Society of America2010

Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy

Tristan Colomb, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Pierre Magistretti, Benjamin Rappaz, Viesturs Simanis, Elena Cano

Digital holography microscopy (DHM) is an optical technique which provides phase images yielding quantitative information about cell structure and cellular dynamics. Furthermore, the quantitative phase images allow the derivation of other parameters, inclu ...

SPIE Society of Photo-optical Instrumentation Engineers2009

Submicrometer tomography of cells by multiple-wavelength digital holographic microscopy in reflection

Tristan Colomb, Frédéric Montfort, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Nicolas Pavillon, Benjamin Rappaz, Corinne Moratal

We present first results on a method enabling mechanical scanning-free tomography with submicrometer axial resolution by multiple-wavelength digital holographic microscopy. By sequentially acquiring reflection holograms and summing 20 wavefronts equally sp ...

2009

Method and apparatus to measure and compute the amplitude point spread function and associated parameters of a coherent optical imaging system

Tristan Colomb, Christian Depeursinge, Pierre Marquet, Florian Charrière, Mihaela Marian

The invention concerns a method of and an apparatus for the measurement of the Amplitude Point Spread Function (APSF), of an Optical Imaging System (OIS), wherein the APSF is defined as the complex field distribution of the beam delivered by the OIS and or ...

2008

Axial sub-nanometer accuracy in digital holographic microscopy

Tristan Colomb, Frédéric Montfort, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Florian Charrière

2008

On the complex three-dimensional amplitude point spread function of lenses and microscope objectives: theoretical aspects, simulations and measurements by digital holography

Tristan Colomb, Frédéric Montfort, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Florian Charrière, Mihaela Marian

The point spread function is widely used to characterize the three-dimensional imaging capabilities of an optical system. Usually, attention is paid only to the intensity point spread function, whereas the phase point spread function is most often neglecte ...

2007

Real-time dual-wavelength digital holographic microscopy with a single hologram acquisition

Tristan Colomb, Frédéric Montfort, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Florian Charrière

A technique to perform two-wavelengths digital holographic microscopy (DHM) measurements with a single hologram acquisition is presented. The vertical measurement range without phase ambiguity is extended to the micron-range, thanks to the resulting synthe ...

2007

Amplitude point spread function measurement of high NA microscope objectives by digital holographic microscopy

Tristan Colomb, Christian Depeursinge, Pierre Marquet, Florian Charrière, Mihaela Marian

We present here a three-dimensional evaluation of the amplitude point-spread function (APSF) of a microscope objective (MO), based on a single holographic acquisition of its pupil wavefront. The aberration function is extracted from this pupil measurements ...

2007

Influence of shot noise on phase measurement accuracy in digital holographic microscopy

Tristan Colomb, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Florian Charrière, Benjamin Rappaz

Digital Holographic Microscopy (DHM) is a single shot interferometric technique, which provides quantitative phase images with subwavelength axial accuracy. A short hologram acquisition time (down to microseconds), allows DHM to offer a reduced sensitivity ...

2007

Digital holographic microscopy applied to life sciences

Tristan Colomb, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Florian Charrière, Benjamin Rappaz

Digital holographic microscopy (DHM) is applied to life sciences applications and demonstrate its capability of real-time imaging and quantitative measurements of physiological parameters such as cell volume or mean cell hemoglobin concentration (MCHC) of ...

IEEE2007

WAVE FRONT SENSING METHOD AND APPARATUS

Tristan Colomb, Frédéric Montfort, Stéphane Bourquin, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Nicolas Aspert, Florian Charrière, Mihaela Marian

A new way of mixing instrumental and digital means is described for the general field of wave front sensing. The present invention describes the use, the definition and the utility of digital operators, called digital wave front operators (DWFO) or ... A n ...

2006

Cell refractive index tomography by digital holographic microscopy

Tristan Colomb, Frédéric Montfort, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Florian Charrière, Mihaela Marian

For what we believe to be the first time, digital holographic microscopy is applied to perform optical diffraction tomography of a pollen grain. Transmission phase images with nanometric axial accuracy are numerically reconstructed from holograms acquired ...

2006

Numerical aberrations compensation and polarization imaging in digital holographic microscopy

Tristan Colomb

In this thesis, we describe a method for the numerical reconstruction of the complete wavefront properties from a single digital hologram: the amplitude, the phase and the polarization state. For this purpose, we present the principle of digital holographi ...

EPFL2006

Total aberrations compensation in digital holographic microscopy with a reference conjugated hologram

Tristan Colomb, Christian Depeursinge, Pierre Marquet, Nicolas Aspert, Jonas Kühn, Florian Charrière

In this paper we present a new method to achieve quantitative phase contrast imaging in Digital Holographic Microscopy (DHM) that allows to compensate for phase aberrations and image distortion by recording of a single reference hologram.We demonstrate tha ...

2006

Automatic procedure for aberration compensation in digital holographic microscopy and applications to specimen shape compensation

Tristan Colomb, Frédéric Montfort, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Nicolas Aspert, Jonas Kühn, Florian Charrière

We present a procedure that compensates for phase aberrations in digital holographic microscopy by computing a polynomial phase mask directly from the hologram. The phase-mask parameters are computed automatically without knowledge of physical values such ...

2006

Numerical parametric lens for shifting, magnification and complete aberration compensation in digital holographic microscopy

Tristan Colomb, Frédéric Montfort, Stéphane Bourquin, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Nicolas Aspert, Jonas Kühn, Florian Charrière, Mihaela Marian

The concept of numerical parametric lenses (NPL) is introduced to achieve wavefront reconstruction in digital holography. It is shown that operations usually performed by optical components and described in ray geometrical optics, such as image shifting, m ...

2006

Living specimen tomography by digital holographic microscopy: morphometry of testate amoeba

Tristan Colomb, Christian Depeursinge, Pierre Marquet, Florian Charrière, Nicolas Pavillon, Edward Mitchell, Benjamin Rappaz, Thierry Jean Heger

This paper presents an optical diffraction tomography technique based on digital holographic microscopy. Quantitative 2-dimensional phase images are acquired for regularly-spaced angular positions of the specimen covering a total angle of π, allowing to bu ...

2006

Characterization of microlenses by digital holographic microscopy

Tristan Colomb, Frédéric Montfort, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Florian Charrière

We demonstrate the use of digital holographic microscopy (DHM) as a metrological tool in micro-optics testing. Measurement principles are compared with those performed with Twyman-Green, Mach-Zehnder, and white-light interferometers. Measurements performed ...

2006