Publications by Arnaud Comment

Correlated cryo-SEM and CryoNanoSIMS imaging of biological tissue

Arnaud Comment, Cristina Martin Olmos, Tian Cheng, Anders Meibom, Stéphane Laurent Escrig, Florent Olivier Vivien Plane, Louise Helene Soegaard Jensen, Nils Rädecker

BackgroundThe development of nanoscale secondary ion mass spectrometry (NanoSIMS) has revolutionized the study of biological tissues by enabling, e.g., the visualization and quantification of metabolic processes at subcellular length scales. However, the a ...

2023

[13C]bicarbonate labelled from hyperpolarized [1-13C]pyruvate is an in vivo marker of hepatic gluconeogenesis in fasted state

Arnaud Comment, Rolf Gruetter, Josefina Adriana Maria Bastiaansen, Emine Can, Hikari Ananda Infinity Yoshihara

Hyperpolarized [1-13C]pyruvate enables direct in vivo assessment of real-time liver enzymatic activities by 13C magnetic resonance. However, the technique usually requires the injection of a highly supraphysiological dose of pyruvate. We herein demonstrate ...

2022

Assessment of Aspartate and Bicarbonate Produced From Hyperpolarized [1-13C]Pyruvate as Markers of Renal Gluconeogenesis

Arnaud Comment, Hikari Ananda Infinity Yoshihara

As both a consumer and producer of glucose, the kidney plays a significant role in glucose homeostasis. Measuring renal gluconeogenesis requires invasive techniques, and less invasive methods would allow renal gluconeogenesis to be measured more routinely. ...

2021

Hyperpolarized C-13-glucose magnetic resonance highlights reduced aerobic glycolysis in vivo in infiltrative glioblastoma

Arnaud Comment, Bernard Lanz, Rolf Gruetter, Cristina Ramona Cudalbu, Mor-Miri Mishkovsky, Jocelyne Bloch

Glioblastoma (GBM) is the most aggressive brain tumor type in adults. GBM is heterogeneous, with a compact core lesion surrounded by an invasive tumor front. This front is highly relevant for tumor recurrence but is generally non-detectable using standard ...

Nature Research2021

A method to disentangle and quantify host anabolic turnover in photosymbiotic holobionts with subcellular resolution

Arnaud Comment, Anders Meibom, Maoz Fine, Emma Mary Gibbin, Guilhem Maurice Louis Banc-Prandi

A wide range of organisms host photosynthesizing symbionts. In these animals the metabolic exchange between host and symbionts has prevented in situ host anabolic turnover to be studied without the confounding effect of translocated photosynthates. Using t ...

2020

Noninvasive rapid detection of metabolic adaptation in activated human T lymphocytes by hyperpolarized 13C magnetic resonance

Arnaud Comment, Nicolas Kunz, Mor-Miri Mishkovsky, Emine Can, Hikari Ananda Infinity Yoshihara

The metabolic shift induced in human CD4+ T lymphocytes by stimulation is characterized by an upregulation of glycolysis, leading to an augmentation in lactate production. This adaptation has already been highlighted with various techniques and reported in ...

Nature Research2020

Detection of myocardial medium‐chain fatty acid oxidation and tricarboxylic acid cycle activity with hyperpolarized [1– 13 C]octanoate

Arnaud Comment, Josefina Adriana Maria Bastiaansen, Hikari Ananda Infinity Yoshihara

Under normal conditions, the heart mainly relies on fatty acid oxidation to meet its energy needs. Changes in myocardial fuel preference are noted in the diseased and failing heart. The magnetic resonance signal enhancement provided by spin hyperpolarizati ...

2020

Sample preparation for CryoNanoSIMS

Arnaud Comment, Tian Cheng, Anders Meibom, Stéphane Laurent Escrig, Florent Olivier Vivien Plane, Louise Helene Soegaard Jensen

2019

Probing cardiac metabolism by hyperpolarized 13C MR using an exclusively endogenous substrate mixture and photo-induced nonpersistent radicals

Arnaud Comment, Rolf Gruetter, Josefina Adriana Maria Bastiaansen, Andrea Capozzi, Hikari Ananda Infinity Yoshihara

Purpose To probe the cardiac metabolism of carbohydrates and short chain fatty acids simultaneously in vivo following the injection of a hyperpolarized 13C-labeled substrate mixture prepared using photo-induced nonpersistent radicals. Methods Droplets of m ...

Wiley2018

Hyperpolarized C-13 Magnetic Resonance Spectroscopy Reveals the Rate-Limiting Role of the Blood-Brain Barrier in the Cerebral Uptake and Metabolism of L-Lactate in Vivo

Arnaud Comment, Sylvain Eric Lengacher, Bernard Lanz, Mor-Miri Mishkovsky, Tian Cheng, Josefina Adriana Maria Bastiaansen, Yuhei Takado, Hikari Ananda Infinity Yoshihara

The dynamics of L-lactate transport across the blood-brain barrier (BBB) and its cerebral metabolism are still subject to debate. We studied lactate uptake and intracellular metabolism in the mouse brain using hyperpolarized C-13 magnetic resonance spectro ...

2018

Thermal annihilation of photo-induced radicals following dynamic nuclear polarization to produce transportable frozen hyperpolarized 13C-substrates

Arnaud Comment, Giovanni Boero, Christophe Roussel, Tian Cheng, Andrea Capozzi

Hyperpolarization via dynamic nuclear polarization (DNP) is pivotal for boosting magnetic resonance imaging (MRI) sensitivity and dissolution DNP can be used to perform in vivo real-time 13C MRI. The type of applications is however limited by the relativel ...

Nature Publishing Group2017

Pyruvate cellular uptake and enzymatic conversion probed by dissolution DNP-NMR: the impact of overexpressed membrane transporters

Arnaud Comment, Paul Romeo Vasos, Riccardo Balzan

Pyruvate membrane crossing and its lactate dehydrogenase-mediated conversion to lactate in cells featuring different levels of expression of membrane monocarboxylate transporters (MCT4) were probed by dissolution dynamic nuclear polarization-enhanced NMR. ...

Wiley2017

Hyperpolarized MRS: New tool to study real-time brain function and metabolism

Arnaud Comment, Mor-Miri Mishkovsky

The advent of dissolution dynamic nuclear polarization (DNP) led to the emergence of a new kind of magnetic resonance (MR) measurements providing the opportunity to probe metabolism in vivo in real time. It has been shown that, following the injection of h ...

Elsevier2017

A new passive system for contamination-free long-distance cryo-transfer of biological tissues

Arnaud Comment, Tian Cheng, Anders Meibom, Florent Olivier Vivien Plane, Louise Helene Soegaard Jensen

2017

Measuring glucose cerebral metabolism in the healthy mouse using hyperpolarized C-13 magnetic resonance

Arnaud Comment, Rolf Gruetter, Mor-Miri Mishkovsky

The mammalian brain relies primarily on glucose as a fuel to meet its high metabolic demand. Among the various techniques used to study cerebral metabolism, C-13 magnetic resonance spectroscopy (MRS) allows following the fate of C-13-enriched substrates th ...

Springer Nature2017

Direct Monitoring of γ-Glutamyl Transpeptidase Activity In Vivo Using a Hyperpolarized (13) C-Labeled Molecular Probe

Arnaud Comment, Josefina Adriana Maria Bastiaansen, Yuhei Takado, Emine Can, Hikari Ananda Infinity Yoshihara

The γ-glutamyl transpeptidase (GGT) enzyme plays a central role in glutathione homeostasis. Direct detection of GGT activity could provide critical information for the diagnosis of several pathologies. We propose a new molecular probe, γ-Glu-[1-(13) C]Gly, ...

Wiley-Blackwell2016

LRH-1-dependent programming of mitochondrial glutamine processing drives liver cancer

Arnaud Comment, Matthias Alexander Sokrates Stein, Kristina Schoonjans, Johan Auwerx, Norman Lionel Moullan, Dongryeol Ryu, Maaike Hélène Oosterveer, Emine Can, Pan Xu, Xu Wang, Hadrien Charles Edouard Demagny

Various tumors develop addiction to glutamine to support uncontrolled cell proliferation. Here we identify the nuclear receptor liver receptor homolog 1 (LRH-1) as a key regulator in the process of hepatic tumorigenesis through the coordination of a noncan ...

Cold Spring Harbor Laboratory2016

Hyperpolarized 6Li as a probe for hemoglobin oxygenation level

Arnaud Comment, Rolf Gruetter, Ruud van Heeswijk, Riccardo Balzan, Mor-Miri Mishkovsky

Hyperpolarization by dissolution dynamic nuclear polarization (DNP) is a versatile technique to dramatically enhance the nuclear magnetic resonance (NMR) signal intensity of insensitive long-T1 nuclear spins such as 6 Li. The 6 Li longitudinal relaxation o ...

Wiley-Blackwell2016

High-field dissolution dynamic nuclear polarization of [1-C-13]pyruvic acid

Arnaud Comment, Emine Can, Hikari Ananda Infinity Yoshihara

[1-C-13]pyruvate is the most widely used hyperpolarized metabolic magnetic resonance imaging agent. Using a custom-built 7.0 T polarizer operating at 1.0 K and trityl radical-doped [1-C-13] pyruvic acid, unextrapolated solution-state C-13 polarization grea ...

Royal Soc Chemistry2016

En route to ion microprobe analysis of soluble compounds at single cell level: The CryoNanoSIMS

Arnaud Comment, Tian Cheng, Anders Meibom, Stéphane Laurent Escrig, Florent Olivier Vivien Plane, Louise Helene Soegaard Jensen

2016