Publications by Charles Roduit

A universal fluid cell for the imaging of biological specimens in the atomic force microscope

Charles Roduit, Giovanni Dietler, Sandor Kasas, Livan Bladimir Alonso Sarduy, Giovanni Longo, Bhaskar Saha

Recently, atomic force microscope (AFM) manufacturers have begun producing instruments specifically designed to image biological specimens. In most instances, they are integrated with an inverted optical microscope, which permits concurrent optical and AFM ...

Wiley-Blackwell2013

Stiffness tomography exploration of living and fixed macrophages

Charles Roduit, Giovanni Dietler, Sandor Kasas, Andrea Volterra, Giovanni Longo, Bhaskar Saha

Stiffness tomography is a new atomic force microscopy imaging technique that allows highlighting structures located underneath the surface of the sample. In this imaging mode, such structures are identified by investigating their mechanical properties. We ...

Wiley-Blackwell2012

Force volume and stiffness tomography investigation on the dynamics of stiff material under bacterial membranes

Charles Roduit, Giovanni Dietler, Sandor Kasas, Giovanni Longo, Laura Rio Marques

The determination of the characteristics of micro-organisms in clinical specimens is essential for the rapid diagnosis and treatment of infections. A thorough investigation of the nanoscale properties of bacteria can prove to be a fundamental tool. Indeed, ...

Wiley-Blackwell2012

Atomic Force Microscopy Stiffness Tomography on Living Arabidopsis thaliana Cells Reveals the Mechanical Properties of Surface and Deep Cell-Wall Layers during Growth

Charles Roduit, Giovanni Dietler, Sandor Kasas, Christian Fankhauser

Cell-wall mechanical properties play a key role in the growth and the protection of plants. However, little is known about genuine wall mechanical properties and their growth-related dynamics at subcellular resolution and in living cells. Here, we used ato ...

Elsevier2012

Differential peptide binding to CD40 evokes counteractive responses

Charles Roduit, Giovanni Dietler, Sandor Kasas, Livan Bladimir Alonso Sarduy, Bhaskar Saha

The antigen-presenting cell-expressed CD40 is implied in the regulation of counteractive immune responses such as induction of pro-inflammatory and anti-inflammatory cytokines interleukin (IL)-12 and IL-10, respectively. The mechanism of this duality in CD ...

Elsevier2012

Differential peptide binding to CD40 evokes counteractive responses (vol 73, pg 465, 2012)

Charles Roduit, Giovanni Dietler, Sandor Kasas, Livan Bladimir Alonso Sarduy, Bhaskar Saha

Elsevier2012

A Role for Septins in the Interaction between the Listeria monocytogenes Invasion Protein InlB and the Met Receptor

Charles Roduit, Sandor Kasas

Septins are conserved GTPases that form filaments and are required for cell division. During interphase, septin filaments associate with cellular membrane and cytoskeleton networks, yet the functional significance of these associations have, to our knowled ...

Elsevier2011

Human topoisomerase II-DNA interaction study by using atomic force microscopy

Charles Roduit, Giovanni Dietler, Sandor Kasas, Livan Bladimir Alonso Sarduy

AFM; DNA topology; Human topoisomerase II; Binding geometry; Time-lapse imaging; Protein–DNA interaction ...

Elsevier2011

Atomic Force Microscopy Imaging of Living Cells

Charles Roduit, Sandor Kasas

Cambridge, Cambridge University Press2010

Stiffness Tomography by Atomic Force Microscopy

Charles Roduit, Giovanni Dietler, Sandor Kasas, Serguei Sekatski

The atomic force microscope is a convenient tool to probe living samples at the nanometric scale. Among its numerous capabilities, the instrument can be operated as a namo-indenter to gather information about the mechanical properties of the sample. In thi ...

2009

Membrane elastic heterogeneity studied at nanometrical scale on living cells

Charles Roduit

The aim of this thesis was to explore the cell mechanical properties using the Atomic Force Microscope (AFM). The cell membrane contains lipids microdomains, called rafts, enriched in cholesterol and sphingolipids. The rafts are believed to play an importa ...

EPFL2008

Elastic membrane heterogeneity of living cells revealed by stiff nanoscale membrane domains

Charles Roduit, Giovanni Dietler, Paolo De Los Rios, Sandor Kasas, Pascal Steiner, Alexandre Yersin, Françoise Gisou van der Goot Grunberg

Many approaches have been developed to characterize the heterogeneity of membranes in living cells. In this study, the elastic properties of specific membrane domains in living cells are characterized by atomic force microscopy. Our data reveal the existen ...

Elsevier2008

Stiffness tomography by atomic force microscopy

Charles Roduit, Sandor Kasas

A method of analyzing a sample with a probe based measurement instrument using stiffness tomography. Stiffness or elastic modulus data obtained through contact measurement with the sample as a function of depth is used in the detection of features, particu ...

2007