Publications by Philippe Renaud

Rapid exocytosis kinetics measured by amperometry within volcano microelectrodes

Philippe Renaud, Arnaud Bertsch, Nicolas Grégoire Maïno

For over 30 years, carbon fiber microelectrodes have been the gold standard for measurements related to exocytosis and more generally to the processes taking place at the synaptic level. However, this method has a low throughput and molecules can escape de ...

ROYAL SOC CHEMISTRY2023

A Three-Dimensional Engineered Cardiac In Vitro Model: Controlled Alignment of Cardiomyocytes in 3D Microphysiological Systems

Philippe Renaud, Fatemeh Navaee

Cardiomyocyte alignment in myocardium tissue plays a significant role in the physiological, electrical, and mechanical functions of the myocardium. It remains, however, difficult to align cardiac cells in a 3D in vitro heart model. This paper proposes a si ...

MDPI2023

Microfluidic device combining hydrodynamic and dielectrophoretic trapping for the controlled contact between single micro-sized objects and application to adhesion assays

Philippe Renaud, Arnaud Bertsch, Clémentine Sophie Sarah Lipp

The understanding of cell-cell and cell-matrix interactions via receptor and ligand binding relies on our ability to study the very first events of their contact. Of particular interest is the interaction between a T cell receptor and its cognate peptide-m ...

ROYAL SOC CHEMISTRY2023

Toward a Physiologically Relevant 3D Helicoidal-Oriented Cardiac Model: Simultaneous Application of Mechanical Stimulation and Surface Topography

Philippe Renaud, Niccolo Piacentini, Fatemeh Navaee

Myocardium consists of cardiac cells that interact with their environment through physical, biochemical, and electrical stimulations. The physiology, function, and metabolism of cardiac tissue are affected by this dynamic structure. Within the myocardium, ...

MDPI2023

Breaking one into three: Surface-tension-driven droplet breakup in T-junctions

Philippe Renaud, Arnaud Bertsch, François Gallaire, Daniel Migliozzi, Yves-Marie François Ducimetière, Jiande Zhou, Ludovic Pierre Gustave Keiser

Droplet breakup is an important phenomenon in the field of microfluidics to generate daughter droplets. In this work, a novel breakup regime in the widely studied T-junction geometry is reported, where the pinch-off occurs laterally in the two outlet chann ...

AMER PHYSICAL SOC2023

Microfluidic device for sample encapsulation and method for operation thereof

Philippe Renaud, Jiande Zhou

The invention relates to a microfluidic device (3) comprising: - an inlet channel (31) having an inlet configured to be operatively connected with a droplet source (2) wherein the inlet channel (31) has a width wi and height hi; and - an outlet channel wit ...

2023

Aperture-Controlled Fabrication of All-Dielectric Structural Color Pixels

Philippe Renaud, Arnaud Bertsch, Olivier Martin, Daniel Migliozzi, Clémentine Sophie Sarah Lipp, Evgenii Glushkov, Hsiang-Chu Wang, Audrey Zoé Marie Jacquillat

While interference colors have been known for a longtime, conventionalcolor filters have large spatial dimensions and cannot be used tocreate compact pixelized color pictures. Here we report a simple yetelegant interference-based method of creating microsc ...

AMER CHEMICAL SOC2023

Engineered substrates incapable of induction of chondrogenic differentiation compared to the chondrocyte imprinted substrates

Philippe Renaud, Shahin Bonakdar, Leila Montazeri, Zahrasadat Ghazali

It is well established that surface topography can affect cell functions. However, finding a reproducible and reliable method for regulating stem cell behavior is still under investigation. It has been shown that cell imprinted substrates contain micro- an ...

IOP Publishing Ltd2023

Highly Efficient Cardiac Differentiation and Maintenance by Thrombin-Coagulated Fibrin Hydrogels Enriched with Decellularized Porcine Heart Extracellular Matrix

Philippe Renaud, Thomas Michael Braschler, Fatemeh Navaee

Biochemical and biophysical properties instruct cardiac tissue morphogenesis. Here, we are reporting on a blend of cardiac decellularized extracellular matrix (dECM) from porcine ventricular tissue and fibrinogen that is suitable for investigations employi ...

MDPI2023

Impedance spectroscopy of the cell/nanovolcano interface enables optimization for electrophysiology

Philippe Renaud, Arnaud Bertsch, Nicolas Grégoire Maïno

Volcano-shaped microelectrodes have demonstrated superior performance in measuring attenuated intracellular action potentials from cardiomyocyte cultures. However, their application to neuronal cultures has not yet yielded reliable intracellular access. Th ...

SPRINGERNATURE2023

Traceable impedance-based single-cell pipetting, from a research set-up to a robust and fast automated robot: DispenCell-S1

Philippe Renaud

Single-cell isolation is a truly transformative tool for the understanding of biological systems. It allows single-cell molecular analyses and considers the heterogeneity of cell populations, which is of particular relevance for the diagnosis and treatment ...

ELSEVIER SCIENCE INC2022

High precision, high throughput generation of droplets containing single cells

Philippe Renaud, Arnaud Bertsch, Jiande Zhou

The Poisson limit is a major problem for the isolation of single cells in different single-cell technologies and applications. In droplet-based single-cell assays, a scheme that is increasingly popular, the intrinsic randomness during single-cell encapsula ...

ROYAL SOC CHEMISTRY2022

Nanometer-sensitive wideband opto-mechanical switching concept

Philippe Renaud, Dorian Giraud Herle

The present paper is concerned with the mechanically extremely sensitive reflection switching concept of a free-space wave impinging on an array of dielectric or semiconductor pillars. Splitting the pillars of a 2D periodic array in its resonant reflection ...

IOP Publishing Ltd2022

Dielectrophoretic Traps for Efficient Bead and Cell Trapping and Formation of Aggregates of Controlled Size and Composition

Philippe Renaud, Arnaud Bertsch, Clémentine Sophie Sarah Lipp

We present a microfluidic dielectrophoretic-actuated system designed to trap chosen single-cell and form controlled cell aggregates. A novel method is proposed to characterize the efficiency of the dielectrophoretic trapping, considering the flow speed but ...

2022

Method and system for determining intracellular mediators' activity

Horst Vogel, Philippe Renaud, Thamani Dahoun, Margaux Catherine Marie Duchamp, Marc Brugarolas Campillos

A method for determining the presence of a molecule coupled to the cytoplasmic side of a cellular membrane is disclosed. The method is implemented in a microfluidic setting and is particularly suitable for determining the presence and/or the activity of a ...

2022

First steps towards interlocking modular microfluidic cooling substrates (i-MμCS) for future silicon tracking detectors in High Energy Physics (HEP)

Philippe Renaud, Massimo Angeletti

In future High Energy Physics detectors, the coverage of large surfaces with silicon pixel chip sensors poses a challenge for the sensors positioning, for their cooling, assembly, and interconnection. The use of a cooling substrate on which the sensors are ...

2022

Microscale hydrodynamic confinements: shaping liquids across length scales as a toolbox in life sciences

Philippe Renaud, David Philipp Taylor, Prerit Mathur

Hydrodynamic phenomena can be leveraged to confine a range of biological and chemical species without needing physical walls. In this review, we list methods for the generation and manipulation of microfluidic hydrodynamic confinements in free-flowing liqu ...

ROYAL SOC CHEMISTRY2022

On-Demand Nanoliter Sampling Probe for the Collection of Brain Fluid

Philippe Renaud, Arnaud Bertsch, Hilal Lashuel, Daniel Ortiz Trujillo, Laure Menin, Joan Teixidor, Salvatore Novello

Continuous fluidic sampling systems allow collection of brain biomarkers in vivo. Here, we propose a new sequential and intermittent sampling paradigm using droplets, called Droplet on Demand (DoD). It is implemented in a microfabricated neural probe and a ...

AMER CHEMICAL SOC2022

Microfluidic Device for Droplet Pairing by Combining Droplet Railing and Floating Trap Arrays

Philippe Renaud, Margaux Catherine Marie Duchamp, George Coukos, Alexandre Harari, Marion Arnaud

Droplet microfluidics are characterized by the generation and manipulation of discrete volumes of solutions, generated with the use of immiscible phases. Those droplets can then be controlled, transported, analyzed or their content modified. In this wide d ...

MDPI2021

Neural priming of adipose-derived stem cells by cell-imprinted substrates*

Philippe Renaud, Omid Mashinchian

Cell-imprinting technology is a novel method for directing stem cell fate using substrates molded from target cells. Here, we fabricated and studied cell-imprinted substrates for neural priming in human adipose-derived stem cells in the absence of chemical ...

IOP PUBLISHING LTD2021