Publications by Thomas Lehnert

Microfluidic systems for infectious disease diagnostics

Microorganisms, encompassing both uni- and multicellular entities, exhibit remarkable diversity as omnipresent life forms in nature. They play a pivotal role by supplying essential components for sustaining biological processes across diverse ecosystems, i ...

Royal Soc Chemistry2024

Bubble-enhanced ultrasonic microfluidic chip for rapid DNA fragmentation (vol 22, pg 560, 2022)

Thomas Lehnert, Martinus Gijs, Lin Sun

ROYAL SOC CHEMISTRY2023

Polydimethylsiloxane microstructure-induced acoustic streaming for enhanced ultrasonic DNA fragmentation on a microfluidic chip

Thomas Lehnert, Martinus Gijs, Lin Sun

Next-generation sequencing (NGS) is an essential technology for DNA identification in genomic research. DNA fragmentation is a critical step for NGS and doing this on-chip is of great interest for future integrated genomic solutions. Here we demonstrate fa ...

ROYAL SOC CHEMISTRY2022

The enhancement of DNA fragmentation in a bench top ultrasonic water bath with needle-induced air bubbles: Simulation and experimental investigation

Thomas Lehnert, Martinus Gijs, Yang Liu, Lin Sun

Shearing DNA to a certain size is the first step in many medical and biological applications, especially in next-generation gene sequencing technology. In this article, we introduced a highly efficient ultrasonic DNA fragmentation method enhanced by needle ...

AIP Publishing2022

Bubble-enhanced ultrasonic microfluidic chip for rapid DNA fragmentation

Thomas Lehnert, Martinus Gijs, Lin Sun

DNA fragmentation is an essential process in developing genetic sequencing strategies, genetic research, as well as for the diagnosis of diseases with a genetic signature like cancer. Efficient on-chip DNA fragmentation protocols would be beneficial to pro ...

ROYAL SOC CHEMISTRY2022

Antimicrobial susceptibility testing by measuring bacterial oxygen consumption on an integrated platform

Thomas Lehnert, Martinus Gijs, Yang Liu

Cellular respiration is a fundamental feature of metabolic activity and oxygen consumption can be considered as a reliable indicator of bacterial aerobic respiration, including for facultative anaerobic bacteria like E. coli. Addressing the emerging global ...

ROYAL SOC CHEMISTRY2021

An In Vivo Microfluidic Study of Bacterial Load Dynamics and Absorption in the C. elegans Intestine

Thomas Lehnert, Martinus Gijs, Vittorio Viri, Maël Arveiler

Caenorhabditiselegans (C. elegans) has gained importance as a model for studying host-microbiota interactions and bacterial infections related to human pathogens. Assessing the fate of ingested bacteria in the worm's intestine is therefore of great interes ...

MDPI2021

Effect of inoculum size and antibiotics on bacterial traveling bands in a thin microchannel defined by optical adhesive

Thomas Lehnert, Martinus Gijs

Phenotypic diversity in bacterial flagella-induced motility leads to complex collective swimming patterns, appearing as traveling bands with transient locally enhanced cell densities. Traveling bands are known to be a bacterial chemotactic response to self ...

SPRINGERNATURE2021

Force microscopy of the Caenorhabditis elegans embryonic eggshell

Thomas Lehnert, Martinus Gijs, Roger Philippe Krenger

Assays focusing on emerging biological phenomena in an animal's life can be performed during embryogenesis. While the embryo of Caenorhabditis elegans has been extensively studied, its biomechanical properties are largely unknown. Here, we demonstrate that ...

NATURE PUBLISHING GROUP2020

Fast antimicrobial susceptibility testing onEscherichia coliby metabolic heat nanocalorimetry

Thomas Lehnert, Martinus Gijs, Yang Liu

Fast spreading of antimicrobial resistance is now considered a major global health threat. New technologies are required, enabling rapid diagnostics of bacterial infection combined with fast antimicrobial susceptibility testing (AST) for evaluating the eff ...

2020

Anin vivomicrofluidic study of bacterial transit inC. elegansnematodes

Thomas Lehnert, Martinus Gijs, Matteo Cornaglia, Hüseyin Baris Atakan, Vittorio Viri

Caenorhabditis elegans(C. elegans) constitutes an important model organism for use in nutrition and aging studies. We report a novel method for studying the dynamics ofEscherichia coli(E. coli) bacterial transit through the worms' intestine. A microfluidic ...

2020

Microfluidic system for Caenorhabditis elegans culture and oxygen consumption rate measurements

Thomas Lehnert, Martinus Gijs, Matteo Cornaglia, Roger Philippe Krenger

Mitochondrial respiration is a key signature for the assessment of mitochondrial functioning and mitochondrial dysfunction is related to many diseases including metabolic syndrome and aging-associated conditions. Here, we present a microfluidic Caenorhabdi ...

ROYAL SOC CHEMISTRY2020

Dynamic microfluidic nanocalorimetry system for measuring Caenorhabditis elegans metabolic heat

Thomas Lehnert, Martinus Gijs, Roger Philippe Krenger

2018

Integrated Microfluidic Device for Drug Studies of Early C. Elegans Embryogenesis

Thomas Lehnert, Martinus Gijs, Pierre Gönczy, Matteo Cornaglia, Li Dong, Radek Jankele

2018

Reversible and long-term immobilization in a hydrogel-microbead matrix for high-resolution imaging of Caenorhabditis elegans and other small organisms

Thomas Lehnert, Martinus Gijs, Johan Auwerx, Matteo Cornaglia, Laurent Mouchiroud, Li Dong, Govindan Narayanan Krishnamani, Jingwei Zhang

2018

8-Channel Single Embryo Pipette For Accurate C-Elegans Bioassays

Thomas Lehnert, Martinus Gijs, Li Dong, Jingwei Zhang

The nematode Caenorhabditis elegans (C. elegans) is a powerful model organism for addressing fundamental biological questions related to human disease and aging. Accurate control of the number of worms is a primary step for quantitative biological or bioch ...

IEEE2018

An Integrated Microfluidic Device For C-Elegans Early Embryogenesis Studies And Drug Assays

Thomas Lehnert, Martinus Gijs, Pierre Gönczy, Matteo Cornaglia, Li Dong, Radek Jankele, Jingwei Zhang

The Caenorhabditis elegans embryo is a widely used model for the functional analysis of dynamic cellular processes, such as chromosome segregation, cytokinesis or lineage analysis. However, the conventional embryo preparation method that relies on manually ...

Ieee2017

Microfluidic systems for high-throughput and high-content screening using the nematode Caenorhabditis elegans

Thomas Lehnert, Martinus Gijs, Matteo Cornaglia

In a typical high-throughput drug screening (HTS) process, up to millions of chemical compounds are applied to cells cultured in well plates, aiming to find molecules that exhibit a robust dose-response, as evidenced for example by a fluorescence signal. I ...

Royal Soc Chemistry2017

C. Elegans Immobilization Using Deformable Microfluidics For In Vivo Studies Of Early Embryogenesis And Intestinal Microbiota

Thomas Lehnert, Martinus Gijs, Matteo Cornaglia, Li Dong, Xiaopeng Huang, Jingwei Zhang

The genome of the nematode Caenorhabditis elegans shows far-reaching similarities to that of humans. C. elegans is therefore considered as a powerful model organism in biological research related to humans, for instance for the study of human diseases. Suc ...

Ieee2017

Track C. Biomedical Instrumentation & Micro and Nano Systems

Thomas Lehnert, Martinus Gijs, Johan Auwerx, Shreya Narasimhan, Matteo Cornaglia, Laurent Mouchiroud, Alexis Pierre Henri Marette, Virginija Jovaisaite, Govindan Narayanan Krishnamani, Vincenzo Sorrentino

De Gruyter2016