Publications by Marcel Leutenegger

Spectral cross-cumulants for multicolor super-resolved SOFI imaging

Marcel Leutenegger, Theo Lasser, Aleksandra Radenovic, Adrien Charles-François Raymond Descloux, Tomas Lukes, Kristin Stefanie Grussmayer

Super-resolution opticalfluctuation imaging provides a resolution beyond the diffraction limitby analysing stochasticfluorescencefluctuations with higher-order statistics. Usingnthorderspatio-temporal cross-cumulants the spatial resolution and the sampling ...

Nature Research2020

Combined Multi-Plane Tomographic Phase Retrieval and Stochastic Optical Fluctuation Imaging for 4D Cell Microscopy

Marcel Leutenegger, Theo Lasser, Hilal Lashuel, Adrien Charles-François Raymond Descloux, Emrah Bostan, Arno Pino Bouwens, Anne-Laure Mahul Mellier, Tomas Lukes

Super-resolution fluorescence microscopy provides unprecedented insight into cellular and subcellular structures. However, going beyond the diffraction barrier comes at a price since most far-field super-resolution imaging techniques trade temporal for spa ...

Nature Publishing Group2018

Complementarity of PALM and SOFI for super-resolution live-cell imaging of focal adhesions

Marcel Leutenegger, Theo Lasser, Aleksandra Radenovic, Lely Feletti, Daniel Pawel Szlag, Azat Sharipov, Hendrik Georges Deschout, Tomas Lukes

Live-cell imaging of focal adhesions requires a sufficiently high temporal resolution, which remains a challenge for super-resolution microscopy. Here we address this important issue by combining photoactivated localization microscopy (PALM) with super-res ...

Nature Publishing Group2016

Optical prism for creating multiple views of an image

Marcel Leutenegger, Theo Lasser, Stefan Geissbuehler

Optical prism comprising two external parallel and reflecting surfaces, one internal interface, parallel to said surfaces and acting as light splitter; said prism furthermore comprising an entrance side forming an angle β with respect to said interface and ...

2016

Diffraction-unlimited imaging: from pretty pictures to hard numbers

Marcel Leutenegger, Theo Lasser

Diffraction-unlimited fluorescence imaging allows the visualization of intact, strongly heterogeneous systems at unprecedented levels of detail. Beyond the acquisition of detailed pictures, increasing efforts are now being focused on deriving quantitative ...

Springer Verlag2015

Optical coherence correlation spectroscopy (OCCS)

Marcel Leutenegger, Theo Lasser, Stéphane Broillet, Stefan Geissbühler, Christophe Pache, Arno Pino Bouwens, Akihiro Sato

We present a new method called optical coherence correlation spectroscopy (OCCS) using nanoparticles as reporters of kinetic processes at the single particle level. OCCS is a spectral interferometry based method, thus giving simultaneous access to several ...

Optical Society of America2014

Live-cell multiplane three-dimensional super-resolution optical fluctuation imaging

Marcel Leutenegger, Theo Lasser, Aurélien Godinat, Françoise Gisou van der Goot Grunberg, Patrick Alain Sandoz, Noelia Laura Bocchio, Azat Sharipov, Stefan Geissbuehler

Super-resolution optical fluctuation imaging (SOFI) provides an elegant way of overcoming the diffraction limit in all three spatial dimensions by computing higher-order cumulants of image sequences of blinking fluorophores acquired with a classical widefi ...

Nature Research2014

Visible light optical coherence correlation spectroscopy

Heinrich Hofmann, Marcel Leutenegger, Theo Lasser, Stéphane Broillet, Arno Pino Bouwens, Lionel André Maurizi, Daniel Pawel Szlag

Optical coherence correlation spectroscopy (OCCS) allows studying kinetic processes at the single particle level using the backscattered light of nanoparticles. We extend the possibilities of this technique by increasing its signal-to-noise ratio by a fact ...

Optical Society of America2014

Mapping molecular statistics with balanced super-resolution optical fluctuation imaging (bSOFI)

Marcel Leutenegger, Theo Lasser, Claudio Dellagiacoma, Corinne Berclaz, Noelia Laura Bocchio, Stefan Geissbuehler

Super-resolution optical fluctuation imaging (SOFI) achieves 3D super-resolution by computing temporal cumulants or spatio-temporal cross-cumulants of stochastically blinking fluorophores. In contrast to localization microscopy, SOFI is compatible with wea ...

2012

Triplet imaging of oxygen consumption during the contraction of a single smooth muscle cell (A7r5)

Iwan Märki, Marcel Leutenegger, Theo Lasser, Kai Johnsson, Matthias Geissbühler, Dimitri Nestor Alice Van De Ville, Boris Hinz, Thiemo Spielmann, Aurélie Formey de Saint Louvent

Triplet imaging is a novel optical technique that allows investigating oxy- gen metabolism at the single cell and the sub-cellular level. The method combines high temporal and spatial resolution, which are required for the monitoring of fast kinetics of ox ...

Springer Science+Business Media2012

Zero-crossing approach to high-resolution reconstruction in frequency-domain optical-coherence tomography

Marcel Leutenegger, Theo Lasser, Chandra Sekhar Seelamantula, Arno Pino Bouwens

We address the problem of high-resolution reconstruction in frequency-domain optical-coherence tomography (FDOCT). The traditional method employed uses the inverse discrete Fourier transform, which is limited in resolution due to the Heisenberg uncertainty ...

2012

Real-time Full Field Laser Doppler Imaging

Marcel Leutenegger, Theo Lasser, Tyler Nerton Thacher

We present a full field laser Doppler imaging instrument that enables real-time in vivo assessment of blood flow in dermal tissue and skin. The instrument monitors the blood perfusion in an area of about 50cm2 with 480 x 480 pixels per frame at a rate of 1 ...

Amer Inst Physics2012

Nonlinear Correlation Spectroscopy (NLCS)

Iwan Märki, Marcel Leutenegger, Theo Lasser, Jean-Pierre Wolf, Luigi Bonacina, Noelia Laura Bocchio, Stefan Geissbuehler

We present a novel concept for optical spectroscopy called nonlinear correlation spectroscopy (NLCS). NLCS analyses coherent field fluctuations of the second and third harmonic light generated by diffusing nanoparticles. Particles based on noncentrosymmetr ...

American Chemical Society2012

Real-time full field laser Doppler imaging

Marcel Leutenegger, Theo Lasser, Antonio Lopez, Tyler Nerton Thacher, Erica Martin-Williams

We present a full field laser Doppler imaging instrument, which enables real-time in vivo assessment of blood flow in dermal tissue and skin. This instrument monitors the blood perfusion in an area of about 50 cm² with 480 × 480 pixels per frame at a rate ...

2011

Functional optical coherent imaging platform for medical application e.g. neurosurgery, has active camera unit that captures image of specific region of test subject after transmitting stimulus signals

Iwan Märki, Marcel Leutenegger, Theo Lasser, Martin Villiger, Michael Friedrich, Dimitri Nestor Alice Van De Ville, Erica Martin-Williams

The present invention relates to a functional optical coherent imaging (fOCI) platform comprising at least one active camera unit (ACU) comprising a coherent and/or a partially coherent light source, and means for spectral filtering and imaging a selected ...

2010

Method for displaying field e.g. tumor, of brain of human patient during surgery of brain, involves acquiring topography data representing topography of field, where output image is displayed based on topography data

Marcel Leutenegger, Theo Lasser, Antonio Lopez, Erica Martin-Williams

NOVELTY - The method involves illuminating a field (11) of brain (13) of a patient for acquiring an image sequence of the field. The field is illuminated after stimulating the patient for acquiring another image sequence. The image sequences are evaluated ...

2010

Object i.e. aneurysm, examining method for brain of patient, involves evaluating sequence of images by associating pixels with analysis image value depending on temporal changes of image values of pixels in specified frequency range

Marcel Leutenegger, Theo Lasser, Antonio Lopez, Michael Friedrich, Erica Martin-Williams

NOVELTY - The method involves illuminating an object i.e. aneurysm, with measuring light with wavelengths ranging from 790 nanometer to 820 nanometer, and recording a sequence of images (21, 22) i.e. white light image, of the illuminated object. The sequen ...

2009

High volume confinement in two-photon total-internal- reflection fluorescence correlation spectroscopy

Iwan Märki, Marcel Leutenegger, Theo Lasser, Denis Ivanov

We report results on two-photon total-internal-reflection fluorescence correlation spectroscopy with radially polarized light. The combination of liquid crystal spatial light modulator, providing radial polarization, with ultrafast laser (picosecond Nd:GdV ...

AIP American Institute of Physics2009

Imaging of G protein-coupled receptors in solid-supported planar lipid membranes

Marcel Leutenegger, Theo Lasser

This contribution summarizes first some of our efforts in imaging G-protein-coupled receptor (GPCR) functional inserted into planar tethered lipid bilayer membranes (tBLMs) as a novel platform for biophysical studies. The authors introduced recently a nove ...

2009

See the Brain at Work—Intraoperative Laser Doppler Functional Brain Imaging

Marcel Leutenegger, Michaël Unser, Theo Lasser, Antonio Lopez, Dimitri Nestor Alice Van De Ville, Rainer Leitgeb, Erica Martin-Williams

During open brain surgery we acquire perfusion images non-invasively using laser Doppler imaging. The regions of brain activity show a distinct signal in response to stimulation providing intraoperative functional brain maps of remarkably strong contrast. ...

2009