Publications by Jonas Kühn | EPFL Graph Search

Label-Free Cytotoxicity Screening Assay by Digital Holographic Microscopy

Gerardo Turcatti, Christian Depeursinge, Marc Chambon, Jonas Kühn, Pierre Magistretti, Benjamin Rappaz, Julien Mena, Jérôme Parent, Etienne Shaffer

We introduce a label-free technology based on digital holographic microscopy (DHM) with applicability for screening by imaging, and we demonstrate its capability for cytotoxicity assessment using mammalian living cells. For this first high content screenin ...

Mary Ann Liebert2013

Enhanced robustness digital holographic microscopy for demanding environment of space biology

Christian Depeursinge, Jonas Kühn, Muhammed Fatih Toy, Marcel Egli, Stéphane Richard

We describe an optimized digital holographic microscopy system (DHM) suitable for high-resolution visualization of living cells under conditions of altered macroscopic mechanical forces such as those that arise from changes in gravitational force. Experime ...

2012

Digital holographic reflectometry

Tristan Colomb, Christian Depeursinge, Jonas Kühn, Nicolas Pavillon, Ahmet Ata Akatay

Digital holographic microscopy (DHM) is an interferometric technique that allows real-time imaging of the entire complex optical wavefront (amplitude and phase) reflected by or transmitted through a sample. To our knowledge, only the quantitative phase is ...

2010

Endoscopic low coherence topography measurement for upper airways and hollow samples

Christian Depeursinge, Jonas Kühn, Yves Delacrétaz, Nicolas Pavillon, Etienne Shaffer, Florian Lang

To evaluate the severity of airway pathologies, quantitative dimensioning of airways is of utmost importance. Endoscopic vision gives a projective image and thus no true scaling information can be directly deduced from it. In this article, an approach base ...

SPIE Society of Photo-optical Instrumentation Engineers2010

Digital holographic microscopy real-time monitoring of cytoarchitectural alterations during simulated microgravity

Christian Depeursinge, Jonas Kühn, Jérôme Parent, Christophe Pache, Muhammed Fatih Toy, Marcel Egli

Previous investigations on mammalian cells have shown that microgravity, either that experienced in space, or simulated on earth, causes severe cellular modifications that compromise tissue determination and function. The aim of this study is to investigat ...

SPIE Society of Photo-optical Instrumentation Engineers2010

Extended depth-of-focus by digital holographic microscopy

Tristan Colomb, Etienne Cuche, Christian Depeursinge, Jonas Kühn, Nicolas Pavillon

A recurrent problem in microscopy is the finite depth-of-focus linked to the NA of microscope objectives. Digital holographic microscopy (DHM) has the unique feature of being able to numerically change the focus from a single hologram without the need of m ...

Optical Society of America2010

Cell Morphology and Intracellular Ionic Homeostasis explored with a Multimodal Approach combining Epifluorescence and Digital Holographic Microscopy

Christian Depeursinge, Pierre Marquet, Jonas Kühn, Pierre Magistretti, Nicolas Pavillon, Pascal Jourdain, Daniel Boss, Corinne Moratal, Aleksandr Benke

The authors have developed a live-cell multimodality microscope combining epifluorescence with digital holographic microscopy; it has been implemented with a decoupling procedure allowing to separately measure from the quantitative phase important cell par ...

2010

Multiple-wavelength digital holographic microscopy

Jonas Kühn

In this thesis work, we extend digital holographic microscopy (DHM) capabilities and applications range by using from two to twenty different wavelength laser sources, either simultaneously or sequentially. As an overview, we present methods to increase th ...

EPFL2009

Digital holographic microscopy investigation of second harmonic generated at a glass/air interface

Christian Depeursinge, Jonas Kühn, Nicolas Pavillon, Etienne Shaffer

Optical second harmonic generation, thanks to its coherent nature, is a suitable signal for interferometric measurements, such as digital holography: a well-established imaging technique that allows recovering complex diffraction wavefields from which it i ...

2009

Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy

Tristan Colomb, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Pierre Magistretti, Benjamin Rappaz, Viesturs Simanis, Elena Cano

Digital holography microscopy (DHM) is an optical technique which provides phase images yielding quantitative information about cell structure and cellular dynamics. Furthermore, the quantitative phase images allow the derivation of other parameters, inclu ...

SPIE Society of Photo-optical Instrumentation Engineers2009

Submicrometer tomography of cells by multiple-wavelength digital holographic microscopy in reflection

Tristan Colomb, Frédéric Montfort, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Nicolas Pavillon, Benjamin Rappaz, Corinne Moratal

We present first results on a method enabling mechanical scanning-free tomography with submicrometer axial resolution by multiple-wavelength digital holographic microscopy. By sequentially acquiring reflection holograms and summing 20 wavefronts equally sp ...

2009

Suppression of the zero-order in off-axis digital holography through nonlinear filtering

Christian Depeursinge, Michaël Unser, Jonas Kühn, Nicolas Pavillon, Chandra Sekhar Seelamantula

We present experimental validation of a new reconstruction method for off-axis digital holographic microscopy (DHM). This method effectively suppresses the object auto-correlation — namely the zero-order term — from holographic data, thereby improving the ...

2009

Axial sub-nanometer accuracy in digital holographic microscopy

Tristan Colomb, Frédéric Montfort, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Florian Charrière

2008

On the complex three-dimensional amplitude point spread function of lenses and microscope objectives: theoretical aspects, simulations and measurements by digital holography

Tristan Colomb, Frédéric Montfort, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Florian Charrière, Mihaela Marian

The point spread function is widely used to characterize the three-dimensional imaging capabilities of an optical system. Usually, attention is paid only to the intensity point spread function, whereas the phase point spread function is most often neglecte ...

2007

Influence of shot noise on phase measurement accuracy in digital holographic microscopy

Tristan Colomb, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Florian Charrière, Benjamin Rappaz

Digital Holographic Microscopy (DHM) is a single shot interferometric technique, which provides quantitative phase images with subwavelength axial accuracy. A short hologram acquisition time (down to microseconds), allows DHM to offer a reduced sensitivity ...

2007

Digital holographic microscopy applied to life sciences

Tristan Colomb, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Florian Charrière, Benjamin Rappaz

Digital holographic microscopy (DHM) is applied to life sciences applications and demonstrate its capability of real-time imaging and quantitative measurements of physiological parameters such as cell volume or mean cell hemoglobin concentration (MCHC) of ...

IEEE2007

Real-time dual-wavelength digital holographic microscopy with a single hologram acquisition

Tristan Colomb, Frédéric Montfort, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Florian Charrière

A technique to perform two-wavelengths digital holographic microscopy (DHM) measurements with a single hologram acquisition is presented. The vertical measurement range without phase ambiguity is extended to the micron-range, thanks to the resulting synthe ...

2007

Submicrometer optical tomography by multiple-wavelength digital holographic microscopy

Tristan Colomb, Frédéric Montfort, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Florian Charrière, Sylvain Herminjard

We present a method for submicrometer tomographic imaging using multiple wavelengths in digital holographic microscopy. This method is based on the recording, at different wavelengths equally separated in the k domain, in off- axis geometry, of the interfe ...

2006

Numerical parametric lens for shifting, magnification and complete aberration compensation in digital holographic microscopy

Tristan Colomb, Frédéric Montfort, Stéphane Bourquin, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Nicolas Aspert, Jonas Kühn, Florian Charrière, Mihaela Marian

The concept of numerical parametric lenses (NPL) is introduced to achieve wavefront reconstruction in digital holography. It is shown that operations usually performed by optical components and described in ray geometrical optics, such as image shifting, m ...

2006

Automatic procedure for aberration compensation in digital holographic microscopy and applications to specimen shape compensation

Tristan Colomb, Frédéric Montfort, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Nicolas Aspert, Jonas Kühn, Florian Charrière

We present a procedure that compensates for phase aberrations in digital holographic microscopy by computing a polynomial phase mask directly from the hologram. The phase-mask parameters are computed automatically without knowledge of physical values such ...

2006