Publications by Nicolas Demierre

Rapid, Sensitive and Real-Time Multiplexing Platform for the Analysis of Protein and Nucleic-Acid Biomarkers

Philippe Renaud, Nicolas Demierre, Raphaël Tornay, David Bernasconi, Lucienne Lagopoulos

We describe a multiplexing technology, named Evalution, based on novel digitally encoded microparticles in microfluidic channels. Quantitative multiplexing is becoming increasingly important for research and routine clinical diagnostics, but fast, easy-to- ...

Amer Chemical Soc2015

Tracking and synchronization of the yeast cell cycle using dielectrophoretic opacity

Philippe Renaud, Nicolas Demierre, Thomas Michael Braschler, Ana Valero

Cell cycle synchronization is an important tool for the study of the cell division stages and signalling. It provides homogeneous cell cultures that are of importance to develop and improve processes such as protein synthesis and drug screening. The main a ...

Royal Society of Chemistry2011

Continuous-flow electrical lysis device with integrated control by dielectrophoretic cell sorting

Philippe Renaud, Nicolas Demierre, Thomas Michael Braschler, Niccolo Piacentini

We present a device capable of electrical cell lysis and evaluation of lysis efficiency in continuous flow using dielectrophoretic cell sorting. We use a combination of AC electrical fields and so-called liquid electrodes to avoid bubble creation at the el ...

Royal Society of Chemistry2010

A miniaturized continuous dielectrophoretic cell sorter and its applications

Philippe Renaud, Nicolas Demierre, Thomas Michael Braschler, Ana Valero

There is great interest in highly sensitive separation methods capable of quickly isolating a particular cell type within a single manipulation step prior to their analysis. We present a cell sorting device based on the opposition of dielectrophoretic forc ...

American Institute of Physics2010

Dielectrophoresis-based particle exchanger for the manipulation and surface functionalization of particles

Heinrich Hofmann, Philippe Renaud, Nicolas Demierre, Raphaël Tornay, Jeffrey Alan Hubbell, Thomas Michael Braschler, Benedikt Steitz, Andrija Finka

We present a microfluidic device where micro- and nanoparticles can be continuously functionalized in flow. This device relies on an element called particle exchanger, which allows for particles to be taken from one medium and exposed to some reagent while ...

2008

Focusing and continuous separation of cells in a microfluidic device using lateral dielectrophoresis

Philippe Renaud, Randoald Müller, Nicolas Demierre, Thomas Michael Braschler

We have fabricated a microfluidic device based on a novel geometrical arrangement of lateral metal electrodes and a patterned insulator. Our device combines the concept of insulator-based “electrodeless” dielectrophoresis with multiple frequencies to achie ...

2008

Continuous-flow separation of cells in a lab-on-a-chip using "liquid electrodes" and multiple-frequency dielectrophoresis

Nicolas Demierre

This thesis reports on the integration of continuous-flow cell separation method for lab-on-a-chip applications. Cell separation methods are widely used in biology to prepare samples prior to analysis. There is a need for a highly sensitive separation meth ...

EPFL2008

Dielectrophoretic sorting on a microfabricated flow cytometer: Label free separation of Babesia bovis infected erythrocytes

Philippe Renaud, Nicolas Demierre, Thomas Michael Braschler

Dielectrophoresis is a method that has demonstrated great potential in cell discrimination and isolation. In this study, the dielectrophoretic sorting of normal and Babesia bovis infected erythrocytes was performed using a microfabricated flow cytometer. S ...

2008

Continuous separation of cells by balanced dielectrophoretic forces at multiple frequencies

Philippe Renaud, Nicolas Demierre, Thomas Michael Braschler

We present a particle-sorting device based on the opposition of dielectrophoretic forces. The forces are generated by an array of electrode chambers located in both sidewalls of a main flow channel. Particles with different dielectric response perceive dif ...

2008

A virtual valve for smooth contamination-free flow switching

Philippe Renaud, Mario Schlund, Harald Van Lintel, Nicolas Demierre, Joël Theytaz, Raphaël Tornay, Thomas Michael Braschler, Anja Kunze, Grazia Loche

We present a channel geometry that allows for clean switching between different inlets of a microchip without any contamination of the inlets or the downstream flow. We drive this virtual valve with a pneumatic pressure setup that minimizes disturbance of ...

2007

Characterization and optimization of liquid electrodes for lateral dielectrophoresis

Philippe Renaud, Harald Van Lintel, Nicolas Demierre, Urban Seger, Olof Pontus Linderholm, Thomas Michael Braschler

Using the concept of insulator-based "electrodeless" dielectrophoresis, we present a novel geometry for shaping electric fields to achieve lateral deviation of particles in liquid flows. The field is generated by lateral planar metal electrodes and is guid ...

2007

Apparatus for manipulating, modifying and characterizing particles in a micro channel

Philippe Renaud, Nicolas Demierre, Urban Seger, Olof Pontus Linderholm, Thomas Michael Braschler

Microfluidic system comprising a space (0207) for containing a liquid and at least one lateral chamber (0201) in communication with said space, said lateral chamber containing a metal electrode (0204). The lateral chamber and the space are designed to be f ...

2007

A simple pneumatic setup for driving microfluidics

Philippe Renaud, Harald Van Lintel, Nicolas Demierre, Joël Theytaz, Lynda Metref, Thomas Michael Braschler

2007

Label-free detection of Babesia bovis infected red blood cells using impedance spectroscopy on microfabricated flow cytometer

Philippe Renaud, Nicolas Demierre, Thomas Michael Braschler

Impedance spectroscopy is a powerful tool for label-free analysis and characterisation of living cells. In this work, we achieved the detection of Babesia bovis infected red blood cells using impedance spectroscopy on a microfabricated flow cytometer. The ...

2007

Continuous separation of cells in a microfluidic device using lateral dielectrophoresis

Philippe Renaud, Nicolas Demierre, Thomas Michael Braschler

2007

Creating structures at the scale of cells on the fly: gentle single cell entrapment on chip

Philippe Renaud, Nicolas Demierre, Urban Seger, Olof Pontus Linderholm, Thomas Michael Braschler

2007