Publications by Nicolas Durand

Nanofluidic biosensor and its use for rapid measurement of biomolecular interactions in solution and methods

Theo Lasser, Nicolas Durand, Yannick Sébastien Fournier

A method and device for the rapid detection of biomolecules (320) diffusing in a nanometer-confined slit (204) is claimed. In particular, the present invention relates to a novel concept of fluidic side apertures (205) that facilitates the filling of the d ...

2011

Apparatus and method for detecting and measuring biomolecular interactions

Theo Lasser, Nicolas Durand

A method and system for the rapid detection of biomolecular interactions, the system comprising a sensing platform which comprises a primary support structure including recesses designed to be located in front of a detection unit, said recesses containing ...

2011

Biomolecular Diffusion in Nanofluidics

Nicolas Durand

Imagine a small integrated biomedical analysis laboratory, connected to your home computer, which would be capable of diagnosing illnesses, a lack of vitamins, or the over-presence of substances from samples of blood, urine or saliva. This hypothetic syste ...

EPFL2010

Direct measurement of diffusing proteins in nanochannels using fluorescence correlation spectroscopy

Philippe Renaud, Arnaud Bertsch, Theo Lasser, Nicolas Durand, Raphaël Goetschmann

Transport of proteins in liquid-filled nanometer-sized apertures with charged surfaces based on both diffusion and convective flow is investigated with the use of fluorescence correlation spectroscopy. The measured fluctuations of fluorescence allow the ev ...

Elsevier2009

Detecting proteins complex formation using steady-state diffusion in a nanochannel

Philippe Renaud, Nicolas Durand

In this work, we present theoretical and experimental studies of nanofluidic channels as a potential biosensor for measuring rapid protein complex formation. Using the specific properties offered by nanofluidics, such as the decrease of effective diffusion ...

2009

Direct Observation of Transitions between Surface-Dominated and Bulk Diffusion Regimes in Nanochannels

Philippe Renaud, Arnaud Bertsch, Theo Lasser, Nicolas Durand, Claudio Dellagiacoma, Raphaël Goetschmann

The diffusion of charged proteins in liquid-filled nanometer-sized apertures with charged surfaces has been investigated with fluorescence correlation spectroscopy (FCS). Based on a two-dimensional (2D) multicomponent diffusion model, key parameters such a ...

2009

Label-free determination of protein-surface interaction kinetics by ionic conductance inside a nanochannel

Philippe Renaud, Nicolas Durand

We have developed a label-free conductometric platform for the rapid measurement of kinetics parameters for the adsorption and desorption of proteins on surfaces. Adsorbed Bovine Serum Albumin (BSA) has been detected electrically with response times in the ...

2009

Label-free determination of protein–surface interaction kinetics by ionic conductance inside a nanochannel

Philippe Renaud, Nicolas Durand

2009

Nanofluidic platform for label-free proteins detection

Philippe Renaud, Nicolas Durand, Fabien Patrick Wildhaber

2009

Rapid Immunoassay Using Steady-State Dispersion Effects in Nanochannels

Philippe Renaud, Nicolas Durand, Ana Valero

We present a novel method for on-chip competitive dispersion immunoassay (DISIA) which aims at measuring the specificity between fluorescently-labeled antibodies (Ab*) and label-free antigens (Ag). We have fabricated a nanofluidic device that allows fast d ...

2008

Direct Measurement of Effective Diffusion Coefficients in Nanochannels Using Steady-State Dispersion Effects

Philippe Renaud, Arnaud Bertsch, Mina Todorova, Nicolas Durand

We present a method to measure effective diffusion coefficients of fluorescently labeled molecules inside a nanofluidic system. Molecules with small diffusion coefficients show a larger lateral dispersion than highly diffusive species, which is counterintu ...

American Institute of Physics2007