Publications by Martin Villiger

Longitudinal three-dimensional visualisation of autoimmune diabetes by functional optical coherence imaging

Theo Lasser, Martin Villiger, Anne Grapin-Botton, Corinne Berclaz, Joan Goulley, Arno Pino Bouwens, Daniel Pawel Szlag, Jérôme Extermann

Aims/hypothesis It is generally accepted that structural and functional quantitative imaging of individual islets would be beneficial to elucidate the pathogenesis of type 1 diabetes. We here introduce functional optical coherence imaging (FOCI) for fast, ...

Springer Verlag2016

Diabetes imaging — quantitative assessment of islets of Langerhans distribution in murine pancreas using extended-focus optical coherence microscopy

Anthony Christopher Davison, Theo Lasser, Martin Villiger, Dimitri Nestor Alice Van De Ville, Anne Grapin-Botton, Corinne Berclaz, Joan Goulley, Erica Martin-Williams, Arno Pino Bouwens

Diabetes is characterized by hyperglycemia that can result from the loss of pancreatic insulin secreting β-cells in the islets of Langerhans. We analyzed ex vivo the entire gastric and duodenal lobes of a murine pancreas using extended-focus Optical Cohere ...

2012

Fast three-dimensional imaging of gold nanoparticles in living cells with photothermal optical lock-in Optical Coherence Microscopy

Christian Santschi, Theo Lasser, Martin Villiger, Corinne Berclaz, Christophe Pache, Matthew Gibson, Joan Goulley, Noelia Laura Bocchio, Arno Pino Bouwens

We introduce photothermal optical lock-in Optical Coherence Microscopy (poli-OCM), a volumetric imaging technique, which combines the depth sectioning of OCM with the high sensitivity of photothermal microscopy while maintaining the fast acquisition speed ...

Optical Society of America2012

Label-free imaging of cerebral β-amyloidosis with extended-focus optical coherence microscopy

Theo Lasser, Martin Villiger, Corinne Berclaz, Christophe Pache, Patrick Fraering, Tristan Bolmont, Mitko Dimitrov, Arno Pino Bouwens

We demonstrate label-free imaging of cerebral β-amyloidosis ex vivo and in a living mouse model of Alzheimer's disease using extended focus Fourier domain optical coherence microscopy (xfOCM). xfOCM provides 3D, high-resolution images of individual β-amylo ...

Society for Neuroscience2012

Dark field optical coherence microscopy

Theo Lasser, Martin Villiger, Christophe Pache

The invention relates to an optical coherence microscopy system for fast, phase resolved imaging by means of optical coherence microscopy with decoupled illumination and detection apertures, producing a dark-field effect with an enhanced optical contrast. ...

2011

Functional optical coherent imaging platform for medical application e.g. neurosurgery, has active camera unit that captures image of specific region of test subject after transmitting stimulus signals

Iwan Märki, Marcel Leutenegger, Theo Lasser, Martin Villiger, Michael Friedrich, Dimitri Nestor Alice Van De Ville, Erica Martin-Williams

The present invention relates to a functional optical coherent imaging (fOCI) platform comprising at least one active camera unit (ACU) comprising a coherent and/or a partially coherent light source, and means for spectral filtering and imaging a selected ...

2010

Dark-field optical coherence microscopy

Theo Lasser, Martin Villiger, Christophe Pache

Dark-field illumination is known to enhance scattering contrast in optical microscopy. We combined this concept with Fourier domain optical coherence microscopy (OCM). The detection and illumination paths are decoupled, and only the scattered light origina ...

Optical Society of America2010

Image formation and tomogram reconstruction in optical coherence microscopy

Theo Lasser, Martin Villiger

In this work we present a model for image formation in optical coherence microscopy. In the spectral domain detection, each wavenumber has a specific coherent transfer function that samples a different part of the object's spatial frequency spectrum. The r ...

2010

Extended Focus Optical Coherence Microscopy : Theory and Application

Martin Villiger

Optical coherence tomography (OCT) is an interferometric imaging technique that can provide depth resolved cross-sectional views of biological tissue. OCT employs light with low temporal and yet high spatial coherence. The sample is illuminated point by po ...

EPFL2010

Towards high resolution optical imaging of beta cells in vivo

Theo Lasser, Martin Villiger, Anne Grapin-Botton, Joan Goulley, Erica Martin-Williams

Endocrine beta cells produce and release insulin in order to tightly regulate glucose homeostasis and prevent metabolic pathologies such as Diabetes Mellitus. Optical imaging has contributed greatly to our current understanding of beta cell structure and f ...

Bentham Science Publishers2010

In vivo imaging of murine endocrine islets of Langerhans with extended-focus optical coherence microscopy

Theo Lasser, Martin Villiger, Michael Friedrich, Rainer Leitgeb, Anne Grapin-Botton, Joan Goulley

Aims/hypothesis Structural and functional imaging of the islets of Langerhans and the insulin-secreting beta cells represents a significant challenge and a long-lasting objective in diabetes research. In vivo microscopy offers a valuable insight into beta ...

Springer Verlag2009

Optical apparatus for dual beam heterodyne Fourier domain optical coherence tomography, has detector that synchronizes phase shift such that sample signal or sample interface moving at arbitrary velocity is detected by phase change

Adrian Bachmann, Theo Lasser, Roland Michaely, Martin Villiger, Rainer Leitgeb

NOVELTY - The apparatus has an optical unit for splitting a source field to produce and combine reference and biomedical sample light fields. An optical and electronic unit records a spectral interference pattern between combined light fields. A controlled ...

2008

Intensity based quantification of fast retinal blood flow in 3D via high resolution resonant Doppler spectral OCT

Adrian Bachmann, Theo Lasser, Roland Michaely, Martin Villiger, Cédric Blatter, Rainer Leitgeb

Resonant Doppler Fourier Domain Optical Coherence Tomography is a functional imaging modality for quantifying fast tissue flow. The method profits from the effect of interference fringe blurring in spectrometer-based FDOCT in the presence of sample motion. ...

Spie-Int Soc Optical Engineering, Po Box 10, Bellingham, Wa 98227-0010 Usa2007

Vectorial reconstruction of retinal blood flow in three dimensions measured with high resolution resonant Doppler Fourier domain optical coherence tomography

Adrian Bachmann, Theo Lasser, Roland Michaely, Martin Villiger, Cédric Blatter, Rainer Leitgeb

Resonant Doppler Fourier domain optical coherence tomography (FDOCT) is a functional imaging tool for extracting tissue flow. The method is based on the effect of interference fringe blurring in spectrometer-based FDOCT, where the path difference between s ...

SPIE Society of Photo-optical Instrumentation Engineers2007

Resonant Doppler flow imaging and optical vivisection of retinal blood vessels

Adrian Bachmann, Theo Lasser, Martin Villiger, Cédric Blatter, Rainer Leitgeb

For Fourier domain optical coherence tomography any sample movement during camera integration causes blurring of interference fringes and as such reduction of sensitivity for flow detection. The proposed method overcomes this problem by phase-matching a re ...

2007

Vectorial retinal blood flow and optical vessel vivisection for 3D angiography with high resolution resonant Doppler OCT

Adrian Bachmann, Theo Lasser, Roland Michaely, Martin Villiger, Cédric Blatter, Rainer Leitgeb

PURPOSE: To perform high speed and completely non-invasive 3D angiography at the optic nerve head region and to reconstruct vectorial 3D blood flow from optically segmented blood vessel structure as a pre-requisite for testing retinal function and physiolo ...

2007

Optical imaging apparatus for sample imaging method e.g. in-vivo measurement of full three-dimensional tissue volume has at least one detector that detects resulting spectral interference pattern at exit of interferometer

Adrian Bachmann, Theo Lasser, Lukas Steinmann, Martin Villiger, Rainer Leitgeb

The invention concerns an optical imaging apparatus comprising (o) a light source (1) , (p) sample holding means, (q) an interferometer, (r) reference means, (s) an objective (8) which is adapted to have its sample side focal plane crossing a sample held i ...

2007

Extended focus depth for Fourier domain optical coherence microscopy

Adrian Bachmann, Theo Lasser, Lukas Steinmann, Martin Villiger, Rainer Leitgeb

We report on a new detection scheme for Fourier domain optical coherence microscopy that exhibits high transverse resolution along an axially extended focal range. Nearly constant transverse resolution of ∼1.5 μm along a focal range of 200 μm is experiment ...

2006