Publications by Hisham Ben Hamidane

Heterolytic N-Calpha Bond Cleavage in Electron Capture and Transfer Dissociation of Peptide Cations

Hisham Ben Hamidane, Yury Tsybin, Anne-Clémence Corminboeuf, Konstantin Zhurov, Matthew Wodrich, Aleksey Vorobyev

Mass spectrometry techniques employing electron capture and electron transfer dissociation represent powerful approaches for the analysis of biological samples. Despite routine employment in analytical fields, the underlying physical processes dictating pe ...

Amer Chemical Soc2012

On the utility of predictive chromatography to complement mass spectrometry based intact protein identification

Hisham Ben Hamidane, Yury Tsybin

The amino acid sequence determines the individual protein three-dimensional structure and its functioning in an organism. Therefore, "reading" a protein sequence and determining its changes due to mutations or post-translational modifications is one of the ...

Springer-Verlag2012

UV radiation induces genome-mediated, site-specific cleavage in viral protein

Hisham Ben Hamidane, Anne Thérèse Sigstam, Michele Cascella, Tamar Kohn, Yury Tsybin, Laure Menin, Krista Rule Wigginton

Much research has been dedicated to understanding the molecular basis of UV damage to biomolecules, yet many questions remain regarding the specific pathways involved. Here we describe a genome-mediated mechanism that causes sitespecific virus protein clea ...

2012

Repeatability and reproducibility of product ion abundances in electron capture dissociation mass spectrometry of peptides

Hisham Ben Hamidane, Yury Tsybin, Aleksey Vorobyev

Site-specific reproducibility and repeatability of electron capture dissociation (ECD) in Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) are of fundamental importance for product ion abundance (PIA)-based peptide and protein struct ...

2011

Structural Preferences of Gas-Phase M2TMP Monomers upon Sequence Variations

Rodolphe Antoine, Hisham Ben Hamidane, Yury Tsybin

The conformations of a number of M2TMP-(22-46) sequence variants have been investigated using ion mobility spectrometry (IMS). Substantial conformational changes were evidenced by IMS upon the variation of a single amino acid in the peptide sequence, with ...

2011

Peptide Structure and Radical Chemistry in the Gas Phase Revealed by Product Ion Abundance Analysis in Electron Capture and Transfer Dissociation Mass Spectrometry

Hisham Ben Hamidane

Peptide and protein ion abundances in mass spectrometry (MS) and tandem mass spectrometry (MS/MS) may provide orthogonal, or complementary, information to their mass measurements. Collision induced dissociation (CID), the most commonly used MS/MS technique ...

EPFL2011

Radical Stability Directs Electron Capture and Transfer Dissociation of Beta-Amino Acids in Peptides

Hisham Ben Hamidane, Yury Tsybin, Aleksey Vorobyev

We report on the characteristics of the radical-ion-driven dissociation of a diverse array of beta-amino acids incorporated into alpha-peptides, as probed by tandem electron-capture and electron-transfer dissociation (ECD/ETD) mass spectrometry. The report ...

2010

Electron Capture Dissociation Product Ion Abundances at the X Amino Acid in RAAAA-X-AAAAK Peptides Correlate with Amino Acid Polarity and Radical Stability

Hisham Ben Hamidane, Yury Tsybin, Aleksey Vorobyev

We present mechanistic studies aimed at improving the understanding of the product ion formation rules in electron capture dissociation (ECD) of peptides and proteins in Fourier transform ion cyclotron resonance mass spectrometry. In particular, we attempt ...

Springer-Verlag2009

Periodic Sequence Distribution of Product Ion Abundances in Electron Capture Dissociation of Amphipathic Peptides and Proteins

Hisham Ben Hamidane, Yury Tsybin

The rules for product ion formation in electron capture dissociation (ECD) mass spectrometry of peptides and proteins remain unclear. Random backbone cleavage probability and the nonspecific nature of ECD toward amino acid sequence have been reported, cont ...

Springer-Verlag2009

Electron Capture and Transfer Dissociation: Peptide Structure Analysis at Different Ion Internal Energy Levels

Hisham Ben Hamidane, Marc Moniatte, Diego Chiappe, Yury Tsybin, Aleksey Vorobyev

We decoupled electron-transfer dissociation (ETD) and collision-induced dissociation of charge-reduced species (CRCID) events to probe the lifetimes of intermediate radical species in ETD-based ion trap tandem mass spectrometry of peptides. Short-lived int ...

Springer-Verlag2009

Linked Metal-cluster Systems: Isolation and Characterization of {anti-[(p-cymene)RuCl]-m-[k 2-P,P';k 1-P''-(PPh2CH2)3CMe]-[AuPt3(CO)3(PCy3)3]}(PF6)2

Hisham Ben Hamidane, Zoltan Beni, Rosario Scopelliti, Paul Joseph Dyson, Andrew Douglas Phillips, Christian Hartinger

The new mixed-metal complex {anti-[(p-cymene)RuCl]-m-[k 2-P,P';k 1-P''-(PPh2CH2)3CMe]-[AuCl]}PF6 and its cluster deriv. {anti-[(p-cymene)RuCl]-m-[k 2-P,P';k 1-P''-(PPh2CH2)3CMe]-[AuPt3(CO)3(PCy3)3]}(PF6)2 have been prepd. and characterized. Notably, NMR sp ...

Springer US2008

High Resolution Mass Spectrometry for Studying the Interactions of Cisplatin with Oligonucleotides

Hisham Ben Hamidane, Paul Joseph Dyson, Yury Tsybin, Christian Hartinger

Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) has been used to probe the interaction of the anticancer drug cisplatin with oligonucleotides. The binding kinetics, the nature of the adducts formed, and the location of the binding s ...

2008