Publications by Yann Barrandon

Low temperature and mTOR inhibition favor human epidermal stem cell maintenance

Juan Ramon Mosig, Fahd Azzabi Zouraq, Kai Johnsson, Yann Barrandon, Ariane Rochat, Thomas Michael Braschler, Johannes Alexander Mosig, Jean-Baptiste Bureau

ELSEVIER SCIENCE INC2023

Low temperature and mTOR inhibition favor stem cell maintenance in human keratinocyte cultures

Fahd Azzabi Zouraq, Kai Johnsson, Yann Barrandon, Ariane Rochat, Thomas Michael Braschler, Johannes Alexander Mosig, Jean-Baptiste Bureau, Mako Kamiya

Adult autologous human epidermal stem cells can be extensively expanded ex vivo for cell and gene therapy. Identifying the mechanisms involved in stem cell maintenance and defining culture conditions to maintain stemness is critical, because an inadequate ...

WILEY2023

The Transcriptional Regulator Prdm1 Is Essential for the Early Development of the Sensory Whisker Follicle and Is Linked to the Beta-Catenin First Dermal Signal

Yann Barrandon, Bernard Moret, Jessica Sordet, Marion Leleu

Prdm1 mutant mice are one of the rare mutant strains that do not develop whisker hair follicles while still displaying a pelage. Here, we show that Prdm1 is expressed at the earliest stage of whisker development in clusters of mesenchymal cells before plac ...

2022

Impedance-Based Single-Cell Pipetting

Philippe Renaud, Yann Barrandon, David Vincent Bonzon, Georges Muller, Jean-Baptiste Bureau, Nicolas Jean Bernard Beuchat, Nicolas Uffer

Many biological methods are based on single-cell isolation. In single-cell line development, the gold standard involves the dilution of cells by means of a pipet. This process is time-consuming as it is repeated over several weeks to ensure clonality. Here ...

SAGE PUBLICATIONS INC2020

Traceable Impedance-Based Dispensing and Cloning of Living Single Cells

Philippe Renaud, Yann Barrandon, Stéphanie Claudinot, Ariane Rochat, David Vincent Bonzon, Georges Muller

Single-cell cloning is essential in stem cell biology, cancer research, and biotechnology. Regulatory agencies now require an indisputable proof of clonality that current technologies do not readily provide. Here, we report a one-step cloning method using ...

2020

Tp63-expressing adult epithelial stem cells cross lineages boundaries revealing latent hairy skin competence

Yann Barrandon, Stéphanie Claudinot, Paola Bonfanti, Ariane Rochat, Daniel-Alexandre Littman, Christèle Gonneau

The formation of hair follicles, a landmark of mammals, requires complex mesenchymal-epithelial interactions and it is commonly believed that embryonic epidermal cells are the only cells that can respond to hair follicle morphogenetic signals in vivo. Here ...

Nature Research2020

Tip connector for fluidic and electrical connection

Philippe Renaud, Yann Barrandon, David Vincent Bonzon, Georges Muller, Jean-Baptiste Bureau, David Forchelet, Nicolas Uffer

A connector for providing both a fluidic and electrical connection is disclosed, said connector having a proximal end, a distal end and an elongated body in between, characterized in that the elongated body has an inner cavity spanning throughout its lengt ...

2018

Methods of derivation and/or propagation of epithelial cells

Yann Barrandon, Andrea Zaffalon

The present invention relates to methods of derivation and/or propagation of epithelial cells from tissue comprising culturing said tissue in media containing an effective amount of a proprotein convertase inhibitor for a period of time allowing the deriva ...

2018

Sensing Tip with Electrical Impedance Sensor

Philippe Renaud, Yann Barrandon, David Vincent Bonzon

The invention relates to a sensing tip (8) comprising a conventional tip (1), such as a pipette tip, which is essentially made of a cavity which communicates with the external environment through an aperture (3) located at the tip distal end; the sensing t ...

2016

Devices, systems and methods for dispensing and analysing particles

Philippe Renaud, Yann Barrandon, David Vincent Bonzon, Jean-Baptiste Bureau, Steve Béguin

The present invention relates to a pipette tip comprising a thin holed membrane at its distal end, which is designed to be adapted with a system comprising at least an impedance analyser and a fluidic actuator to perform the dispensing and analysis of part ...

2016

Chronic inflammation imposes aberrant cell fate in regenerating epithelia through mechanotransduction

Matthias Lütolf, Yann Barrandon, Freddy Radtke, Craig Nowell, Sylke Höhnel, Georg Fantner, Pascal Damian Odermatt, Luca Azzolin

Chronic inflammation is associated with a variety of pathological conditions in epithelial tissues, including cancer, metaplasia and aberrant wound healing. In relation to this, a significant body of evidence suggests that aberration of epithelial stem and ...

Nature Publishing Group2016

Observation device with optical compensation

Philippe Renaud, Yann Barrandon, David Vincent Bonzon, David Forchelet, Steve Béguin

The invention concerns an observation device such as cell culture wells comprising an optical element such as a lens or a filter, or even combinations thereof, for compensating an optical effect induced on a sample contained in the observation device and i ...

2016

Cell motion predicts human epidermal stemness

Yann Barrandon

Image-based identification of cultured stem cells and noninvasive evaluation of their proliferative capacity advance cell therapy and stem cell research. Here we demonstrate that human keratinocyte stem cells can be identified in situ by analyzing cell mot ...

Rockefeller University Press2015

Clonal, Self-Renewing and Differentiating Human and Porcine Urothelial Cells, a Novel Stem Cell Population

Jeffrey Alan Hubbell, Yann Barrandon, Peter Frey, Hans Mattias Larsson, François Gorostidi

Although urothelial progenitor-like cells have been described in the human urinary tract, the existence of stem cells remains to be proven. Using a culture system that favors clonogenic epithelial cell growth, we evaluated and characterized clonal human ur ...

Public Library of Science2014

Human and porcine urothelial stem cells

Jeffrey Alan Hubbell, Yann Barrandon, Peter Frey, Hans Mattias Larsson, François Gorostidi

Wiley-Blackwell2014

Corneal Endothelial Cell Fate Is Maintained by LGR5 Through the Regulation of Hedgehog and Wnt Pathway

Yann Barrandon

Leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5), a target of Wnt signaling, is reportedly a marker of intestine, stomach, and hair follicle stem cells in mice. To gain a novel insight into the role of LGR5 in human corneal tissue, we per ...

Wiley-Blackwell2013

A clonal strategy for ex vivo gene therapy of epidermis

Yann Barrandon, Stéphanie Danièle Hélène Droz-Georget

Mary Ann Liebert, Inc2013

Device for application of medium to human tissue or animal body such as eye, has carrier which is provided with micro-cavity for containing medium in direct contact with object on which medium is to be applied

Hubert van den Bergh, Philippe Renaud, Yann Barrandon, Thomas Michael Braschler, Julien Graber

NOVELTY - The device has a carrier (1) which is provided with micro-cavity (2) for containing medium in direct contact with the object on which the medium is to be applied. Other carrier is provided with ring to protect the medium from environment when the ...

2013

Actin filament dynamics impacts keratinocyte stem cell maintenance

Yann Barrandon

Cultured human epidermal keratinocyte stem cells (holoclones) are crucial for regenerative medicine for burns and genetic disorders. In serial culture, holoclones progressively lose their proliferative capacity to become transient amplifying cells with lim ...

Wiley Open Access2013

Generation and characterization of a Notch1 signaling-specific reporter mouse line

Yann Barrandon, Stéphanie Claudinot, Freddy Radtke, Rajwinder Lehal, Luca Pellegrinet

Signaling through the Notch1 receptor is essential for the control of numerous developmental processes during embryonic life as well as in adult tissue homeostasis and disease. Since the outcome of Notch1 signaling is highly context-dependent, and its prec ...

Wiley-Blackwell2012