Publications by Nicolas Bouche

In vivo transcriptomic profiling using cell encapsulation identifies effector pathways of systemic aging

Nicolas Bouche, Christophe Boss, Patrick Descombes, Filippo De Franceschi, Sylviane Métairon, Jérome Feige, Omid Mashinchian

Sustained exposure to a young systemic environment rejuvenates aged organisms and promotes cellular function. However, due to the intrinsic complexity of tissues it remains challenging to pinpoint niche-independent effects of circulating factors on specifi ...

2022

Pancreatic beta cell based optical biosensor and system for continuous glucose monitoring

Catherine Dehollain, Nicolas Bouche, Christophe Boss, Umberto De Marchi, Bakul Jitendra Vinchhi, Ninad Dilip Agashe

A bio-electronics system that measures cytosolic [Ca2+] in vitro in the INS-1E pancreatic beta-cells using a 430 nm excitation light with the excitation wavelength of the probe being at 435 nm was successfully implemented and tested. We transfected the INS ...

IEEE2019

Optical pancreatic beta cell based biosensor, applications and glucose monitoring

Catherine Dehollain, Nicolas Bouche, Christophe Boss, Umberto De Marchi, Bakul Jitendra Vinchhi

An optical pancreatic beta-cell based biosensor for measuring cytosolic [Ca2+] is presented in this work. An in vitro bio-electronic system is built for testing various applications and characterizing features of the biosensor. A panoply of applications su ...

IEEE2019

Encapsulation device

Nicolas Bouche, Bernard Schneider, Aurélien Lathuilière

2018

Encapsulation of Insulin-Secreting Cells Expressing a Genetically Encoded Fluorescent Calcium Indicator for Cell-Based Sensing In Vivo

Nicolas Bouche, Christophe Boss, Andreas Wiederkehr, Mouna Conrad, Umberto De Marchi, Federico Sizzano

The development of cell-based biosensors that give insight into cell and tissue function in vivo is an attractive technology for biomedical research. Here, the development of a cell line expressing a fluorescent calcium sensor for the study of beta-cell fu ...

Wiley2017

Encapsulation device

Nicolas Bouche, Bernard Schneider, Aurélien Lathuilière

Provided herein is a cell encapsulation device comprising an internal chamber suitable for holding biological agents, wherein the internal chamber is disposed between a first semipermeable layer and a second semi-permeable layer, and the first and second s ...

2016

5PDCell-based immunotherapy combining encapsulation cell technology and irradiated autologous tumor cells: A novel technology platform that is both safe and feasible. Results from first in man trial

Nicolas Bouche

Oxford, Oxford University Press2015

System and Method For In Vivo Delivery of Antibodies and Fragments Thereof

Patrick Aebischer, Nicolas Bouche, Maurizio Molinari

The present invention is directed to devices and methods for in situ delivering an antibody or a fragment thereof to a host. In particular, the invention relates to devices and methods for in situ delivering an antibody or a fragment thereof to patient suf ...

2011

Chronic delivery of antibody fragments using immunoisolated cell implants as a passive vaccination tool

Patrick Aebischer, Nicolas Bouche, Jean-Charles Bensadoun, Maria del Carmen Sandi Perez, Rolf Gruetter, Maria Isabel Cordero Campana, Osiris Marroquin Belaunzaran, Veronica Setola, Maurizio Molinari

BACKGROUND: Monoclonal antibodies and antibody fragments are powerful biotherapeutics for various debilitating diseases. However, high production costs, functional limitations such as inadequate pharmacokinetics and tissue accessibility are the current pri ...

Public Library of Science2011

An in vivo culture system for human embryos using an encapsulation technology: a pilot study

Patrick Aebischer, Nicolas Bouche

ACKGROUND: Animal studies have demonstrated better embryo development in vivo than in vitro. This pilot study tested the feasibility of using a novel in utero culture system (IUCS) to obtain normal human fertilization and embryo development. METHODS: The I ...

Oxford University Press2009

In vivo calcium deposition on polyvinyl alcohol matrix used in hollow fiber cell macroencapsulation devices

Patrick Aebischer, Nicolas Bouche

The encapsulation of genetically modified cells represents a promising approach for the delivery of therapeutic proteins. The functionality of the device is dependent on the characteristics of the biomaterials, the procedures used in its confection and the ...

2004

Glial cell line-derived neurotrophic factor released by synthetic guidance channels promotes facial nerve regeneration in the rat

Patrick Aebischer, Nicolas Bouche

Regeneration of the human facial nerve after lesion is often limited, leading to severe functional impairments, in particular when repair is delayed for several months, when cross-facial nerve grafts have to be performed, or in elderly patients. To improve ...

2002

Nerve growth factor- and neurotrophin-3-releasing guidance channels promote regeneration of the transected rat dorsal root

Patrick Aebischer, Eric Garrison Fine, Nicolas Bouche

Dorsal roots have a limited regeneration capacity after transection. To improve nerve regeneration, the growth-promoting effects of the neurotrophins nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and neurotrophin-3 (NT-3) were evalua ...

2001

Hydrogel-based three-dimensional matrix for neural cells

Patrick Aebischer, Nicolas Bouche

The ability to organize cells in three dimensions (3D) is an important component of tissue engineering. This study sought to develop an extracellular matrix (ECM) equivalent with a physicochemical structure capable of supporting neurite extension from prim ...

1995