Publications by Boris Hinz | EPFL Graph Search

Dispositif et procédé pour l'évaluation de la contraction cellulaire

A method for assessing cell contraction includes adhering contractile cells to an oxidized and cellular adhesion activated surface of a biocompatible silicone elastomer film. The biocompatible silicone elastomer film allows the cells to contract and wrinkl ...

2017

The Nano-Scale Mechanical Properties of the Extracellular Matrix Regulate Dermal Fibroblast Function

Jean-Jacques Meister, Boris Hinz, Josiane Angèle Charlotte Smith-Clerc, Lara Buscemi Estefanell

Changes in the mechanical properties of dermis occur during skin aging or tissue remodeling and affect the activity of resident fibroblasts. With the aim to establish elastic culture substrates that reproduce the variable softness of dermis, we determined ...

Nature Publishing Group2014

The Mechanical Environment Modulates Intracellular Calcium Oscillation Activities of Myofibroblasts

Lysianne Follonier Castella, Boris Hinz, Adriano Garonna

Myofibroblast contraction is fundamental in the excessive tissue remodeling that is characteristic of fibrotic tissue contractures. Tissue remodeling during development of fibrosis leads to gradually increasing stiffness of the extracellular matrix. We pro ...

Public Library Science2013

Triplet imaging of oxygen consumption during the contraction of a single smooth muscle cell (A7r5)

Iwan Märki, Marcel Leutenegger, Theo Lasser, Kai Johnsson, Matthias Geissbühler, Dimitri Nestor Alice Van De Ville, Boris Hinz, Thiemo Spielmann, Aurélie Formey de Saint Louvent

Triplet imaging is a novel optical technique that allows investigating oxy- gen metabolism at the single cell and the sub-cellular level. The method combines high temporal and spatial resolution, which are required for the monitoring of fast kinetics of ox ...

Springer Science+Business Media2012

The Single-Molecule Mechanics of the Latent TGF-beta 1 Complex

Jean-Jacques Meister, Boris Hinz, Josiane Angèle Charlotte Smith-Clerc, Aurélie Formey de Saint Louvent, Lara Buscemi Estefanell, David Ramonet Jimenez

Background: TGF-beta 1 controls many pathophysiological processes including tissue homeostasis, fibrosis, and cancer progression. Together with its latency-associated peptide (LAP), TGF-beta 1 binds to the latent TGF-beta 1-binding protein-1 (LTBP-1), whic ...

Elsevier2011

A novel culture system provides high yields of non-fibrogenic primary dermal fibroblasts for grafting applications

Hicham Majd, Boris Hinz

Amer Soc Cell Biology2011

Tumor cell invasion is promoted by interstitial flow-induced matrix priming by stromal fibroblasts

Boris Hinz, Melody Swartz, Adrian Chung-Kai Shieh

Interstitial flow emanates from tumors into the microenvironment where it promotes tumor cell invasion. Fibroblasts are key constituents of the tumor stroma that modulate the mechanical environment by matrix remodeling and contraction. Here, we explore how ...

American Association for Cancer Research2011

A New Lock-step Mechanism of Matrix Remodelling Based on Subcellular Contractile Events

Jean-Jacques Meister, Lysianne Follonier Castella, Boris Hinz, Lara Buscemi Estefanell

Myofibroblasts promote tissue contractures during fibrotic diseases. To understand how spontaneous changes in the intracellular calcium concentration, Ca2+, contribute to myofibroblast contraction, we analysed both Ca2+ and subcellular contractio ...

Company of Biologists2010

Hypoxia Impairs Skin Myofibroblast Differentiation and Function

Boris Hinz, Giorgio Pietramaggiori

Ischemic wounds are characterized by oxygen levels lower than that of healthy skin ( hypoxia) and poor healing. To better understand the pathophysiology of impaired wound healing, we investigated how switching from high ( 21%) to low ( 2%) oxygen levels di ...

Nature Publishing Group2010

Coated medical device and method of coating a medical device to reduce, fibrosis and capsule formation

Hicham Majd, Boris Hinz, Giorgio Pietramaggiori

Coating for a medical device comprising cell-adhesive proteins having the ability to reduce fibrous reaction, said coating being in the form of separate islets having an individual area which is less than 12 [mu]m2 and wherein the distance between the isle ...

2010

Myofibroblasts work best under stress

Boris Hinz, Pierre Jean Arnould Wipff

Myofibroblasts are reparative connective tissue cells that contribute to the reconstruction of injured tissue by secreting new extracellular matrix and by exerting high contractile force. Deregulation of these activities results in tissue contracture and d ...

Elsevier2009

The covalent attachment of adhesion molecules to silicone membranes for cell stretching applications

Hicham Majd, Jean-Jacques Meister, Boris Hinz, Pierre Jean Arnould Wipff, Lara Buscemi Estefanell

Strain devices with expandable polydimethylsiloxane (PDMS) culture membranes are frequently used to stretch cells in vitro, mimicking mechanically dynamic tissue environments. To immobilize cell-adhesive molecules to the otherwise non-adhesive PDMS substra ...

Elsevier2009

The effect of lactose-conjugated silk biomaterials on the development of fibrogenic fibroblasts

Boris Hinz

Surface properties of implanted biomaterials can cause fibrotic tissue reactions by stimulating differentiation of host fibroblasts into contractile myofibroblasts. Silk fibroin (SF) protein has been used as biomaterial in pure and blended form. however, i ...

Elsevier2008

Myofibroblast communication is controlled by intercellular mechanical coupling

Jean-Jacques Meister, Lysianne Follonier Castella, Sébastien Schaub, Boris Hinz

Neoformation of intercellular adherens junctions accompanies the differentiation of fibroblasts into contractile myofibroblasts, a key event during development of fibrosis and in wound healing. We have previously shown that intercellular mechanical couplin ...

Company of Biologists2008

Integrins and the activation of latent transforming growth factor beta1 - an intimate relationship

Boris Hinz, Pierre Jean Arnould Wipff

Integrins are crucial for the ability of cells to sense mechanical perturbations and to transmit intracellular stress to their environment. We here review the more recently discovered role of integrins in activating the pleiotrophic cytokine transforming g ...

Elsevier2008

Lkb1 is required for TGFβ-mediated myofibroblast differentiation

Boris Hinz, Pierre Jean Arnould Wipff

Inactivating mutations of the tumor-suppressor kinase gene LKB1 underlie Peutz-Jeghers syndrome (PJS), which is characterized by gastrointestinal hamartomatous polyps with a prominent smooth-muscle and stromal component. Recently, it was noted that PJS-typ ...

Company of Biologists2008

Fibrogenic fibroblasts increase intercellular adhesion strength by reinforcing individual OB-cadherin bonds

Andrzej Kulik, Jean-Jacques Meister, Philippe Pittet, Boris Hinz, Kyumin Lee

We have previously shown that the switch from N-cadherin to OB-cadherin expression increases intercellular adhesion between fibroblasts during their transition from a migratory to a fibrogenic phenotype. Using atomic force microscopy we here show that part ...

2008

A novel method of dynamic culture surface expansion improves mesenchymal stem cell proliferation and phenotype

Hicham Majd, Boris Hinz, Pierre Jean Arnould Wipff, Lara Buscemi Estefanell

Repeated passaging in conventional cell culture reduces pluripotency and proliferation capacity of human mesenchymal stem cells (MSC). We introduce an innovative cell culture method whereby the culture surface is dynamically enlarged during cell proliferat ...

2008

Grab, stick, pull and digest: The functional diversity of actin-associated matrix-adhesion structures: Workshop on Invadopodia, Podosomes and Focal Adhesions in Tissue Invasion

Boris Hinz

2008

The myofibroblast - friend or foe in regenerative medicine?

Boris Hinz

2008