Publications by Romain Wyss | EPFL Graph Search

Use of fourier transform infrared spectroscopy analysis of extracellular vesicles isolated from body fluids for diagnosing, prognosing and monitoring pathophysiological states and method therfor

Horst Vogel, Luigino Grasso, Romain Wyss

The method analyzes biological fluids collected from patients, and uses attenuated total reflecting (ATR) sensors and spectral analysis by Fourier transform infrared spectroscopy (FTIR) to form a spectral fingerprint of extracellular vesicles (EVs) isolate ...

2016

Molecular screening of cancer-derived exosomes by surface plasmon resonance spectroscopy

Horst Vogel, Michel Prudent, Niels Erik Lion, Luigino Grasso, Romain Wyss, Davide Demurtas, Ashwin Thampi, Lorenz Weidenauer

We report on a generic method to detect and iden- tify the molecular profile of exosomes either derived from cultured cell lines or isolated from biofluids. Exosomes are nanovesicles shed by cells into their microenvironment and carry the molecular identit ...

Springer Heidelberg2015

A Quantitative Approach to Evaluate the Impact of Fluorescent Labeling on Membrane-Bound HIV-Gag Assembly by Titration of Unlabeled Proteins

Romain Wyss, Julia Gunzenhäuser, Suliana Manley

The assembly process of the human immunodeficiency virus 1 (HIV-1) is driven by the viral polyprotein Gag. Fluorescence imaging of Gag protein fusions is widely performed and has revealed important information on viral assembly. Gag fusion proteins are com ...

Public Library Science2014

X-ray structure of the mouse serotonin 5-HT3 receptor

Rudolf Hovius, Henning Paul-Julius Stahlberg, Horst Vogel, Cédric Deluz, Luigino Grasso, Romain Wyss, Menno Berend Tol, Ghérici Hassaïne

Neurotransmitter-gated ion channels of the Cys-loop receptor family mediate fast neurotransmission throughout the nervous system. The molecular processes of neurotransmitter binding, subsequent opening of the ion channel and ion permeation remain poorly un ...

Nature Publishing Group2014

Molecular and Dimensional Profiling of Highly Purified Extracellular Vesicles by Fluorescence Fluctuation Spectroscopy

Horst Vogel, Luigino Grasso, Romain Wyss, Davide Demurtas, Camille Wolf, Hans Wolfgang Grosse

Cells secrete extracellular vesicles (EVs) into their microenvironment that act as mediators of intercellulard communication under physiological conditions and in this context also actively participate in spreading various diseases. Large efforts are curre ...

Amer Chemical Soc2014

Mechanisms of HsSAS-6 assembly promoting centriole formation in human cells

Romain Wyss, Pierre Gönczy, Debora Keller, Suliana Manley

SAS-6 proteins are thought to impart the ninefold symmetry of centrioles, but the mechanisms by which their assembly occurs within cells remain elusive. In this paper, we provide evidence that the N-terminal, coiled-coil, and C-terminal domains of HsSAS-6 ...

Rockefeller University Press2014

Spontaneous Cdc42 polarization independent of GDI-mediated extraction and actin-based trafficking

Horst Vogel, Romain Wyss, Romano Tobias Wyss

Amer Soc Cell Biology2014

Downscaling the Analysis of Complex Transmembrane Signaling Cascades to Closed Attoliter Volumes

Rudolf Hovius, Horst Vogel, Joachim Piguet, Luigino Grasso, Romain Wyss, Michael Werner, Ghérici Hassaïne

Cellular signaling is classically investigated by measuring optical or electrical properties of single or populations of living cells. Here we show that ligand binding to cell surface receptors and subsequent activation of signaling cascades can be monitor ...

Public Library of Science2013

Nanostructured Surfaces for the Detection of Single-Particles and Single-Molecules

Romain Wyss

Biomarker detection and diagnosis are important steps in the prevention and the treatment of a disease. There is an increasing need for utmost sensitive and robust sensing devices; in this framework, subwavelength (metal) pores could contribute to a concep ...

EPFL2013

A Zeptoliter Volume Meter for Analysis of Single Protein Molecules

Horst Vogel, Christian Santschi, Cédric Deluz, Olivier Martin, Romain Wyss, Ghérici Hassaïne

American Chemical Society (ACS)2012

DNA Strands from Denatured Duplexes are Translocated through Engineered Protein Nanopores at Alkaline pH

Romain Wyss

Nanopores are under development for the detection of a variety of analytes and the investigation of chemical reactions at the single molecule level. In particular, the analysis of nucleic acid molecules is under intense investigation, including the develop ...

American Chemical Society (ACS)2009