Publications by Daniel Boss | EPFL Graph Search

Revealing Assembly of a Pore-Forming Complex Using Single-Cell Kinetic Analysis and Modeling

Felix Naef, Daniel Boss, Mirko Bischofberger, Françoise Gisou van der Goot Grunberg, Mircea Ioan Iacovache

Many biological processes depend on the sequential assembly of protein complexes. However, studying the kinetics of such processes by direct methods is often not feasible. As an important class of such protein complexes, pore-forming toxins start their jou ...

Elsevier2016

Astrocyte swelling in response to neural activation: role of cotransporters

Pierre Marquet, Pierre Magistretti, Pascal Jourdain, Daniel Boss, Kaspar Rothenfusser

Wiley-Blackwell2015

Automated segmentation of multiple red blood cells with digital holographic microscopy

Pierre Marquet, Daniel Boss

We present a method to automatically segment red blood cells (RBCs) visualized by digital holographic microscopy (DHM), which is based on the marker-controlled watershed algorithm. Quantitative phase images of RBCs can be obtained by using off-axis DHM alo ...

SPIE Society of Photo-optical Instrumentation Engineers2013

Developments in digital holographic microscopy to explore the role of astrocytes in brain water homeostasis

Daniel Boss

EPFL2013

Automated quantitative analysis of 3D morphology and mean corpuscular hemoglobin in human red blood cells stored in different periods

Pierre Marquet, Daniel Boss

Quantitative phase (QP) images of red blood cells (RBCs), which are obtained by off-axis digital holographic microscopy, can provide quantitative information about three-dimensional (3D) morphology of human RBCs and the characteristic properties such as me ...

Optical Soc Amer2013

Marker-free phase nanoscopy

Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Nicolas Pavillon, Pascal Jourdain, Daniel Boss, Yann Jérôme Michel Pascal Cotte, Muhammed Fatih Toy

We introduce a microscopic method that determines quantitative optical properties beyond the optical diffraction limit and allows direct imaging of unstained living biological specimens. In established holographic microscopy, complex fields are measured us ...

Nature Publishing Group2013

Measurement of absolute cell volume, osmotic membrane water permeability, and refractive index of transmembrane water and solute flux by digital holographic microscopy

Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Pascal Jourdain, Daniel Boss

A dual-wavelength digital holographic microscope to measure absolute volume of living cells is proposed. The optical setup allows us to reconstruct two quantitative phase contrast images at two different wavelengths from a single hologram acquisition. When ...

SPIE Society of Photo-optical Instrumentation Engineers2013

Simultaneous Optical Recording in Multiple Cells by Digital Holographic Microscopy of Chloride Current Associated to Activation of the Ligand-Gated Chloride Channel GABA(A) Receptor

Christian Depeursinge, Pierre Marquet, Pascal Jourdain, Daniel Boss, Benjamin Rappaz, Corinne Moratal

Chloride channels represent a group of targets for major clinical indications. However, molecular screening for chloride channel modulators has proven to be difficult and time-consuming as approaches essentially rely on the use of fluorescent dyes or invas ...

Public Library of Science2012

Automated statistical quantification of three-dimensional morphology and mean corpuscular hemoglobin of multiple red blood cells

Pierre Marquet, Daniel Boss

In this paper, we present an automated approach to quantify information about three-dimensional (3D) morphology, hemoglobin content and density of mature red blood cells (RBCs) using off-axis digital holographic microscopy (DHM) and statistical algorithms. ...

Optical Society of America2012

Spatially-Resolved Eigenmode Decomposition of Red Blood Cells Membrane Fluctuations Questions the Role of ATP in Flickering

Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Dimitri Nestor Alice Van De Ville, Annick Hoffmann, Daniel Boss, Benjamin Rappaz

Red blood cells (RBCs) present unique reversible shape deformability, essential for both function and survival, resulting notably in cell membrane fluctuations (CMF). These CMF have been subject of many studies in order to obtain a better understanding of ...

Public Library Science2012

Realistic 3D coherent transfer function inverse filtering of complex fields

Christian Depeursinge, Daniel Boss, Cristian Arfire, Yann Jérôme Michel Pascal Cotte, Muhammed Fatih Toy, Shanshan Kou

We present a novel technique for three-dimensional (3D) image processing of complex fields. It consists in inverting the coherent image formation by filtering the complex spectrum with a realistic 3D coherent transfer function (CTF) of a high-NA digital ho ...

Optical Society of America2011

Determination of Transmembrane Water Fluxes in Neurons Elicited by Glutamate Ionotropic Receptors and by the Co-transporters KCC2 and NKCC1: A Digital Holographic Microscopy Study

Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Nicolas Pavillon, Pascal Jourdain, Daniel Boss, Benjamin Rappaz, Corinne Moratal

Digital holographic microscopy (DHM) is a noninvasive optical imaging technique that provides quantitative phase images of living cells. In a recent study, we showed that the quantitative monitoring of the phase signal by DHM was a simple label-free method ...

Society for Neuroscience2011

Recognition and classification of red blood cells using digital holographic microscopy and data clustering with discriminant analysis

Pierre Marquet, Daniel Boss

We propose to apply statistical clustering algorithms on a three-dimensional profile of red blood cells (RBCs) obtained through digital holographic microscopy (DHM). We show that two classes of RBCs stored for 14 and 38 days can be effectively classified. ...

2011

Method and apparatus for 3d object shape and surface topology measurements by contour depth extraction acquired in a single shot

Christian Depeursinge, Yves Delacrétaz, Daniel Boss

The invention relates to a method and apparatus for topological or shape measurement of objects of any size, by using an interferometric technique comprising an optical source, with selectable coherence, allowing contouring from single shot interferograms ...

2010

Cell Morphology and Intracellular Ionic Homeostasis explored with a Multimodal Approach combining Epifluorescence and Digital Holographic Microscopy

Christian Depeursinge, Pierre Marquet, Jonas Kühn, Pierre Magistretti, Nicolas Pavillon, Pascal Jourdain, Daniel Boss, Corinne Moratal, Aleksandr Benke

The authors have developed a live-cell multimodality microscope combining epifluorescence with digital holographic microscopy; it has been implemented with a decoupling procedure allowing to separately measure from the quantitative phase important cell par ...

2010

Optical recording of cell electrical activity by Digital Holographic Microscopy

Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Pascal Jourdain, Daniel Boss, Benjamin Rappaz

2009

Red blood cell structure and dynamics explored with digital holographic microscopy

Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Pascal Jourdain, Daniel Boss, Benjamin Rappaz

2009

Spatial analysis of erythrocyte membrane fluctuations by digital holographic microscopy

Christian Depeursinge, Pierre Marquet, Pierre Magistretti, Annick Hoffmann, Daniel Boss, Benjamin Rappaz

Red blood cell (RBC) membrane fluctuations provide important insights into cell states. We present a spatial analysis of red blood cell membrane fluctuations by using digital holographic Microscopy (DHM). This interferometric and dye-free technique, posses ...

Elsevier2009