Publications by Joseph Rutkowski

Differential role of nicotinamide adenine dinucleotide deficiency in acute and chronic kidney disease

Joseph Rutkowski, Renuga Devi Rajaram, Elena Katsyuba, Johan Auwerx, Adrienne Joëlle Laurence Mottis

Background. Nicotinamide adenine dinucleotide (NAD(+)) is a ubiquitous coenzyme involved in electron transport and a cosubstrate for sirtuin function. NAD(+) deficiency has been demonstrated in the context of acute kidney injury (AKI). Methods. We studied ...

2021

VEGFR-3 Neutralization Inhibits Ovarian Lymphangiogenesis, Follicle Maturation, and Murine Pregnancy

Jeffrey Alan Hubbell, Melody Swartz, Jong Eun Ihm, Joseph Rutkowski, Miriella Carmela Pasquier, Didier Trono, Seung Tae Lee, Witold Waldemar Kilarski, Vivianne Greenwood

Lymphatic vessels surround follicles within the ovary, but their roles in folliculogenesis and pregnancy, as well as the necessity of Lymphangiogenesis in follicle maturation and health, are undefined. We used systemic delivery of mF4-31C1, a specific anta ...

Elsevier Science Inc2013

Normal Dendritic Cell Mobilization to Lymph Nodes under Conditions of Severe Lymphatic Hypoplasia

Melody Swartz, Joseph Rutkowski

To address the requirement for lymphatic capillaries in dendritic cell (DC) mobilization from skin to lymph nodes (LNs), we used mice bearing one inactivated allele of vascular endothelial growth factor receptor 3 (VEGFR3) where skin lymphatic capillaries ...

American Association of Immunologists ; Oxford University Press2013

Impaired Humoral Immunity and Tolerance in K14-VEGFR-3-Ig Mice That Lack Dermal Lymphatic Drainage

Melody Swartz, Joseph Rutkowski, Miriella Carmela Pasquier, Susan Thomas

Lymphatic vessels transport interstitial fluid, soluble Ag, and immune cells from peripheral tissues to lymph nodes (LNs), yet the contribution of peripheral lymphatic drainage to adaptive immunity remains poorly understood. We examined immune responses to ...

American Association of Immunologists ; Oxford University Press2012

Normal dendritic cell mobilization to lymph nodes under conditions of severe lymphatic hypoplasia

Melody Swartz, Joseph Rutkowski

Wiley-Blackwell2012

Dermal collagen and lipid deposition correlate with tissue swelling and hydraulic conductivity in murine primary lymphedema

Melody Swartz, Joseph Rutkowski

Primary lymphedema is a congenital pathology of dysfunctional lymphatic drainage characterized by swelling of the limbs, thickening of the dermis, and fluid and lipid accumulation in the underlying tissue. Two mouse models of primary lymphedema, the Chy mo ...

American Society for Investigative Pathology2010

Transmural flow modulates cell and fluid transport functions of lymphatic endothelium

Melody Swartz, Joseph Rutkowski, Dimana Miteva, James Brandon Dixon, Witold Waldemar Kilarski

RATIONALE: Lymphatic transport of peripheral interstitial fluid and dendritic cells (DCs) is important for both adaptive immunity and maintenance of tolerance to self-antigens. Lymphatic drainage can change rapidly and dramatically on tissue injury or infl ...

American Heart Association2010

Hypercholesterolemic Mice Exhibit Lymphatic Vessel Dysfunction and Degeneration

Melody Swartz, Joseph Rutkowski, Sai Reddy

Lymphatic vessels are essential for lipid absorption and transport. Despite increasing numbers of observations linking lymphatic vessels and lipids, little research has been devoted to address how dysregulation of lipid balance in the blood, ie, dyslipidem ...

American Society for Investigative Pathology2009

Transmural flow modulates cell and fluid transport functions of lymphatic endothelium: An early indicator of injury and inflammation?

Melody Swartz, Joseph Rutkowski, Dimana Miteva, James Brandon Dixon, Witold Waldemar Kilarski

Karger2009

Altered immune responses in mice without initial dermal lymphatics

Melody Swartz, Joseph Rutkowski, Sachiko Hirosue, Susan Thomas

Karger2009

Molecular and mechanical regulators of lymphatic biology

Joseph Rutkowski

Lymphatic vessels exist in nearly all tissues, yet, despite their omnipresence, there remains a large knowledge gap between the described fundamental roles of lymphatic capillaries and our understanding of their functional biology, adaptive ability, and pa ...

EPFL2008

Regulation of lymphatic capillary regeneration by interstitial flow in skin

Melody Swartz, Joseph Rutkowski

Decreased interstitial flow (IF) in secondary lymphedema is coincident with poor physiological lymphatic regeneration. However, both the existence and direction of causality between IF and lymphangiogenesis remain unclear. This is primarily because the rol ...

2007

A driving force for change: interstitial flow as a morphoregulator

Melody Swartz, Joseph Rutkowski

Dynamic stresses that are present in all living tissues drive small fluid flows, called interstitial flows, through the extracellular matrix. Interstitial flow not only helps to transport nutrients throughout the tissue, but also has important roles in tis ...

Elsevier2007

Cooperative and redundant roles of VEGFR-2 and VEGFR-3 signaling in adult lymphangiogenesis

Melody Swartz, Joseph Rutkowski, Miriella Carmela Pasquier

Activation of vascular endothelial growth factor ( VEGF) receptor-3 (VEGFR-3) by VEGF-C initiates lymphangiogenesis by promoting lymphatic proliferation and migration. However, it is unclear whether VEGFR-3 signaling is required beyond these initial stages ...

Federation of American Society of Experimental Biology2007

Characterization of lymphangiogenesis in a model of adult skin regeneration

Melody Swartz, Joseph Rutkowski

To date, adult lymphangiogenesis is not well understood. In this study we describe the evolution of lymphatic capillaries in regenerating skin and correlate lymphatic migration and organization with the expression of matrix metalloproteinases (MMPs), immun ...

2006

Secondary lymphedema in the mouse tail: Lymphatic hyperplasia, VEGF-C upregulation, and the protective role of MMP-9

Melody Swartz, Joseph Rutkowski

Disturbances in the microcirculation can lead to secondary lymphedema, a common pathological condition that, despite its frequency, still lacks a cure. Lymphedema is clinically well described, but while the genetic underpinnings that cause lymphatic malfor ...

2006