Publications by Anne-Laure Gassner

Validation of the Mass-Extraction-Window for Quantitative Methods Using Liquid Chromatography High Resolution Mass Spectrometry

Bertrand Rochat, Anne-Laure Gassner, Laure Menin

A paradigm shift is underway in the field of quantitative liquid chromatography-mass spectrometry (LC-MS) analysis thanks to the arrival of recent high-resolution mass spectrometers (HRMS). The capability of HRMS to perform sensitive and reliable quantific ...

Amer Chemical Soc2016

Treatment with KLEPTOSE (R) CRYSMEB reduces mouse atherogenesis by impacting on lipid profile and Th1 lymphocyte response

Anne-Laure Gassner

The ability of pharmacological agents to target both "classical" risk factors and inflammation may be key for successful outcomes in the prevention and treatment of atherogenesis. Among the promising drugs interfering with cholesterol metabolism, we invest ...

Elsevier Science Inc2015

Analysis of major milk whey proteins by immunoaffinity capillary electrophoresis coupled with MALDI-MS

Hubert Girault, Anne-Laure Gassner, Natalia Gasilova

Two major milk whey proteins, β-lactoglobulin and α-lactalbumin, are among the main cowmilk allergens and can cause allergy even at a very low concentrations. Therefore, these proteins are interesting targets in food analysis, not only for food quality con ...

Wiley-Blackwell2012

Allergy Diagnostics Using Magnetic Beads in a GRAVI-Cell Microfluidic Device

Hubert Girault, Anne-Laure Gassner, Natalia Gasilova

Schweizerische Chemische Gesellschaft2012

Total serum IgE quantification by microfluidic ELISA using magnetic beads

Hubert Girault, Anne-Laure Gassner

The present work reports on the quantification of total IgE in human serum using a microanalytical device whose fluidics is driven by gravity and capillary forces only. Thanks to the eight parallel microchannels in each microchip, calibration and sample an ...

Springer Verlag2012

Magnetic Core Shell Nanoparticles Trapping in a Microdevice Generating High Magnetic Gradient

Hubert Girault, Anne-Laure Gassner

Magnetic core shell nanoparticles (MCSNPs) 30 nm diameter with a magnetic weight of 10% are usually much too small to be trapped in microfluidic systems using classical external magnets. Here, a simple microchip for efficient MCSNPs trapping and release is ...

Royal Society of Chemistry2011

Magnetic Stationary Phases for Protein Adsorption

Anne-Laure Gassner

Magnetic beads (MB) have now proven to be a powerful tool in both research and biomedical applications. They are available in a wide range of sizes (from nm to several µm) and their surface can be modified with molecules having biological specificities and ...

EPFL2011

Bubble cell for magnetic bead trapping in capillary electrophoresis

Hubert Girault, Anne-Laure Gassner

A bubble cell capillary classically used to extend the optical path length for UV-vis detection is employed here to trap magnetic beads. With this system, a large amount of beads can be captured without inducing a strong pressure drop, as it is the case wi ...

Springer Verlag2011

LEPA: From Proteomics to Energy Conversion

Hubert Girault, Anne-Laure Gassner, Fernando Cortes Salazar, Mohammad Reza Pourhaghighi, Manuel Alejandro Méndez Agudelo, Liang Qiao

This review article summarizes the different fields of research at the Laboratoire d'Electrochimie Physique et Analytique at the Ecole Polytechnique Federale de Lausanne. The research areas covered include charge transfer reactions at soft interfaces, bio- ...

2011

Ring magnets for magnetic beads trapping in a capillary

Hubert Girault, Anne-Laure Gassner

This paper introduces the concept of ring magnets for magnetic beads (MBs) trapping in a capillary. Such magnets enable an easy insertion of a capillary simply like a pearl on a string. With this system, high magnetic forces are obtained thanks to the prox ...

Royal Society of Chemistry2011

Magnetic track array for efficient bead capture in microchannels

Hubert Girault, Anne-Laure Gassner, Mélanie Abonnenc

Magnetism-based microsystems, as those dedicated to immunoaffinity separations or (bio)chemical reactions, take benefit of the large surface area-to-volume ratio provided by the immobilized magnetic beads, thus increasing the sensitivity of the analysis. A ...

Springer Verlag2009

Soft Stylus Probes for Scanning Electrochemical Microscopy

Hubert Girault, Anne-Laure Gassner, Mohamad Hojeij, Gunther Wittstock, Fernando Cortes Salazar

A soft stylus microelectrode probe has been developed to carry out scanning electrochemical microscopy (SECM) of rough, tilted, and large substrates in contact mode. It is fabricated by first ablating a microchannel in a polyethylene terephthalate thin fil ...

2009

Magnetic forces produced by rectangular permanent magnets in static microsystems

Hubert Girault, Joël Stéphane Rossier, Anne-Laure Gassner, Mélanie Abonnenc, Hong-Xu Chen

Finite element numerical simulations were carried out in 2D geometries to map the magnetic field and force distribution produced by rectangular permanent magnets as a function of their size and position with respect to a microchannel. A single magnet, two ...

2009

Remarkable Tuning of the Photophysical Properties of Bifunctional Lanthanide tris(Dipicolinates) and its Consequence on the Design of Bioprobes

Jean-Claude Bünzli, Anne-Sophie Chauvin, Anne-Laure Gassner, Céline Rita Duhot

Derivatives of dipicolinic acid with a polyoxyethylene pendant arm at the pyridine 4-position have been functionalized for potential grafting with biological material. Four ligands with different terminal functions (alcohol, methoxy, phtalimide and amine) ...

2008

Capillary electrophoresis immunoassay using magnetic beads

Hubert Girault, Anne-Laure Gassner, Hong-Xu Chen

Protein A-coated magnetic beads (0.3 μm) have been trapped in a small portion of a neutrally coated capillary (50 μm id). Anti-β-lactoglobulin (β-LG) antibodies have then been immobilized on the beads through strong affinity with protein A to subsequently ...

2008