Publications by Joachim Lingner

Oxidative stress at telomeres triggers internal DNA loops, TRF1 dissociation, and TRF2-dependent R-loops

Joachim Lingner, Wareed Ahmed, Thomas Frédéric Lunardi, Thu Trang Nguyen, Eftychia Kyriacou

Telomeres are the nucleoprotein structures at chromosome ends. Telomeres are particularly sensitive to oxidative stress, which can induce telomere damage, shortening, and premature cellular senescence. How oxidative damage influences telomere structure has ...

2025

Spotiflow: accurate and efficient spot detection for fluorescence microscopy with deep stereographic flow regression

Joachim Lingner, Suliana Manley, Gioele La Manno, Irina Khven, Martin Weigert, Eftychia Kyriacou, Can Aztekin, Albert Dominguez Mantes, Georgios Tsissios, Evangelia Skoufa

Identification of spot-like structures in large, noisy microscopy images is a crucial step for many life-science applications. Imaging-based spatial transcriptomics (iST), in particular, relies on the precise detection of millions of transcripts in low sig ...

2025

TERRA long noncoding RNA: At the interphase of telomere damage, rescue and signaling

Joachim Lingner, Eftychia Kyriacou

TERRA long noncoding RNAs play key roles in telomere function and maintenance. They can orchestrate telomeric chromatin remodeling, regulate telomere maintenance by telomerase and homology-directed repair, and they participate in the telomeric DNA damage r ...

CURRENT BIOLOGY LTD2024

The THO complex counteracts TERRA R-loop-mediated telomere fragility in telomerase(+) cells and telomeric recombination in ALT(+) cells

Joachim Lingner, Rita Valador Fernandes

Telomeres are the nucleoprotein structures at the ends of linear chromosomes. Telomeres are transcribed into long non-coding Telomeric Repeat-Containing RNA (TERRA), whose functions rely on its ability to associate with telomeric chromatin. The conserved T ...

OXFORD UNIV PRESS2023

Detection of TERRA R-Loops at Human Telomeres

Joachim Lingner, Marianna Feretzaki, Galina Glousker, Rita Valador Fernandes

R-loops are three-stranded nucleic acid structures composed of a DNA–RNA hybrid and a displaced DNA strand. The long noncoding RNA TERRA forms R-loops at telomeres influencing the telomeric chromatin composition and impacting on telomere maintenance mechan ...

Springer US2022

TFIIH moonlighting at telomeres

Joachim Lingner, Galina Glousker

Although telomeres are essential for chromosome stability, they represent fragile structures in our genome. Telomere shortening occurs during aging in cells lacking telomerase due to the end replication problem. In addition, recent work uncovered that the ...

COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT2022

The human telomeric proteome during telomere replication

Joachim Lingner, Aleksandra Vancevska, Thomas Frédéric Lunardi, Gérald Joseph Paul Lossaint, Chih-Yi Gabriela Lin

Telomere shortening can cause detrimental diseases and contribute to aging. It occurs due to the end replication problem in cells lacking telomerase. Furthermore, recent studies revealed that telomere shortening can be attributed to difficulties of the sem ...

OXFORD UNIV PRESS2021

Challenging endings: How telomeres prevent fragility

Joachim Lingner, Galina Glousker

It has become apparent that difficulties to replicate telomeres concern not only the very ends of eukaryotic chromosomes. The challenges already start when the replication fork enters the telomeric repeats. The obstacles encountered consist mainly of nonca ...

WILEY2021

The makings of TERRA R-loops at chromosome ends

Joachim Lingner, Marianna Feretzaki, Rita Valador Fernandes

Telomeres protect chromosome ends from nucleolytic degradation, uncontrolled recombination by DNA repair enzymes and checkpoint signaling, and they provide mechanisms for their maintenance by semiconservative DNA replication, telomerase and homologous reco ...

TAYLOR & FRANCIS INC2021

A Role for Human DNA Polymerase λ in Alternative Lengthening of Telomeres

Joachim Lingner, Liuh-Yow Chen

Telomerase negative cancer cell types use the Alternative Lengthening of Telomeres (ALT) pathway to elongate telomeres ends. Here, we show that silencing human DNA polymerase (Pol λ) in ALT cells represses ALT activity and induces telomeric stress. In addi ...

2021

PRDX1 Counteracts Catastrophic Telomeric Cleavage Events That Are Triggered by DNA Repair Activities Post Oxidative Damage

Joachim Lingner, Wareed Ahmed

Telomeres are prone to damage inflicted by reactive oxygen species (ROS). Oxidized telomeric DNA and nucleotide substrates inhibit telomerase, causing telomere shortening. In addition, ROS can induce telomeric single-strand DNA breaks (SSBs). The peroxired ...

2020

RAD51-dependent recruitment of TERRA lncRNA to telomeres through R-loops

Joachim Lingner, Thomas Frédéric Lunardi, Marianna Feretzaki, Rita Valador Fernandes

Telomeres-repeated, noncoding nucleotide motifs and associated proteins that are found at the ends of eukaryotic chromosomes-mediate genome stability and determine cellular lifespan1. Telomeric-repeat-containing RNA (TERRA) is a class of long noncoding RNA ...

2020

Human shelterin protein POT1 prevents severe telomere instability induced by homology-directed DNA repair

Joachim Lingner, Anna-Sophia Briod, Galina Glousker

The evolutionarily conserved POT1 protein binds single-stranded G-rich telomeric DNA and has been implicated in contributing to telomeric DNA maintenance and the suppression of DNA damage checkpoint signaling. Here, we explore human POT1 function through g ...

WILEY2020

SMCHD1 promotes ATM-dependent DNA damage signaling and repair of uncapped telomeres

Joachim Lingner, Verena Pfeiffer, Wareed Ahmed, Aleksandra Vancevska, Marianna Feretzaki

Structural maintenance of chromosomes flexible hinge domain-containing protein 1 (SMCHD1) has been implicated in X-chromosome inactivation, imprinting, and DNA damage repair, and mutations in SMCHD1 can cause facioscapulohumeral muscular dystrophy. More re ...

WILEY2020

Expression and differential regulation of human TERRA at several chromosome ends

Joachim Lingner, Patricia Renck Nunes, Marianna Feretzaki

The telomeric long noncoding RNA TERRA has been implicated in regulating telomere maintenance by telomerase and homologous recombination, and in influencing telomeric protein composition during the cell cycle and the telomeric DNA damage response. TERRA tr ...

COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT2019

CSL controls telomere maintenance and genome stability in human dermal fibroblasts

Joachim Lingner, Paris Jafari, Thomas Frédéric Lunardi

Genomic instability is a hallmark of cancer. Whether it also occurs in Cancer Associated Fibroblasts (CAFs) remains to be carefully investigated. Loss of CSL/RBP-J kappa, the effector of canonical NOTCH signaling with intrinsic transcription repressive fun ...

Springer2019

When Telomerase Causes Telomere Loss

Joachim Lingner, Galina Glousker

Telomerase counteracts telomere shortening, preventing cellular senescence. Telomerase deficiency causes telomere syndromes because of premature telomere exhaustion in highly proliferative cells. Paradoxically, in a recent issue of Cell, Margalef et al. (2 ...

Cell Press - Elsevier2018

Joachim Lingner

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Oxidative stress at telomeres triggers internal DNA loops, TRF1 dissociation, and TRF2-dependent R-loops

Spotiflow: accurate and efficient spot detection for fluorescence microscopy with deep stereographic flow regression

TERRA long noncoding RNA: At the interphase of telomere damage, rescue and signaling

The THO complex counteracts TERRA R-loop-mediated telomere fragility in telomerase(+) cells and telomeric recombination in ALT(+) cells

Detection of TERRA R-Loops at Human Telomeres

TFIIH moonlighting at telomeres

The human telomeric proteome during telomere replication

Challenging endings: How telomeres prevent fragility

The makings of TERRA R-loops at chromosome ends

A Role for Human DNA Polymerase λ in Alternative Lengthening of Telomeres

PRDX1 Counteracts Catastrophic Telomeric Cleavage Events That Are Triggered by DNA Repair Activities Post Oxidative Damage

RAD51-dependent recruitment of TERRA lncRNA to telomeres through R-loops

Human shelterin protein POT1 prevents severe telomere instability induced by homology-directed DNA repair

SMCHD1 promotes ATM-dependent DNA damage signaling and repair of uncapped telomeres

Expression and differential regulation of human TERRA at several chromosome ends

CSL controls telomere maintenance and genome stability in human dermal fibroblasts

When Telomerase Causes Telomere Loss

PRDX1 and MTH1 cooperate to prevent ROS-mediated inhibition of telomerase

Impact of oxidative stress on telomere biology

Transformation-induced stress at telomeres is counteracted through changes in the telomeric proteome including SAMHD1