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Sarah Wulhfard

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Related publications (5)

Please note that this is not a complete list of this person’s publications. It includes only semantically relevant works. For a full list, please refer to Infoscience.

Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Sarah Wulhfard

Valproic acid (VPA) is a small molecule that inhibits histone deacetylase activity. Here we report that VPA increases recombinant mRNA and protein levels in transiently transfected CHO DG44 cells. In the presence of VPA, transient recombinant antibody yiel ...

Elsevier2010

Transient recombinant protein expression in mammalian cells : the role of mRNA level and stability

Transient gene expression (TGE) is a rapid method for generating recombinant proteins in mammalian cells, but the volumetric productivities for secreted proteins in transiently transfected CHO DG44 cells are typically more than an order of magnitude lower ...

EPFL2009

Mild hypothermia improves transient gene expression yields several fold in Chinese hamster ovary cells

Florian Maria Wurm, Sophie Bouchet, David Hacker, Stéphanie Tissot, Jean Cevey, Sarah Wulhfard

Large-scale transient gene expression (TGE) in mammalian cells is a rapid method to generate recombinant proteins, but the volumetric productivity for secreted proteins is still more than an order of magnitude lower than the yields typically achieved with ...

2008