Publications by Nathalie Brandenberg

Injury-induced intestinal stem cell renewal requires capillary morphogenesis gene 2

Béatrice Kunz, Olivier Burri, Françoise Gisou van der Goot Grunberg, Nathalie Brandenberg, Julien Léonard Duc, Romain Guiet, Audrey Geneviève Chuat, Lucie Josepha Marie Bracq

Patients with the rare genetic disorder Hyaline Fibromatosis Syndrome (HFS) often succumb before 18 months of age due to severe diarrhea. As HFS is caused by loss-of-function mutations in the gene encoding capillary morphogenesis gene 2 (CMG2), these sympt ...

Springer Science and Business Media LLC2025

Organoid models: the future companions of personalized drug development

Nathalie Brandenberg, Francesco Piraino, Camilla Carla Alma Ceroni

High failure rates of the current drug development process are driving exemplary changes toward methodologies centered on human disease in-vitro modeling. Organoids are self-organized tissue sub-units resembling their organ of origin and are widely acknowl ...

2024

Assessing the precision of morphogen gradients in neural tube development

Matthias Lütolf, Nathalie Brandenberg

Nature Portfolio2024

Probing the killing potency of tumor-infiltrating lymphocytes on microarrayed colorectal cancer tumoroids

Matthias Lütolf, Damien Saugy, Nathalie Brandenberg, François Réal Rivest, Sylke Höhnel, Krisztian Homicsko, Nicolas Antoine Vincent Broguiere, Devanjali Dutta

Immunotherapy has emerged as a new standard of care for certain cancer patients with specific cellular and molecular makeups. However, there is still an unmet need for ex vivo models able to readily assess the effectiveness of immunotherapeutic treatments ...

2024

Fusion-negative rhabdomyosarcoma 3D organoids to predict effective drug combinations: A proof-of-concept on cell death inducers

Nathalie Brandenberg

Rhabdomyosarcoma (RMS) is the main form of pediatric soft-tissue sarcoma. Its cure rate has not notably improved in the last 20 years following relapse, and the lack of reliable preclinical models has hampered the design of new therapies. This is particula ...

Cell Press2023

Rectal organoid-guided CFTR modulator therapy restores lung function in a cystic fibrosis patient with the rare 1677delTA/R334W genotype

Nathalie Brandenberg, Camilla Carla Alma Ceroni

EUROPEAN RESPIRATORY SOC JOURNALS LTD2022

Microarrayed human bone marrow organoids for modeling blood stem cell dynamics

Matthias Lütolf, Nathalie Brandenberg, Romain Guiet, Moritz Hofer, Sonja Giger

In many leukemia patients, a poor prognosis is attributed either to the development of chemotherapy resistance by leukemic stem cells (LSCs) or to the inefficient engraftment of transplanted hematopoietic stem/progenitor cells (HSPCs) into the bone marrow ...

AIP Publishing2022

Tissue geometry drives deterministic organoid patterning

Matthias Lütolf, Nathalie Brandenberg, Nikolche Gjorevski, Mikhail Nikolaev, Olga Mitrofanova

Epithelial organoids are stem cell-derived tissues that approximate aspects of real organs, and thus they have potential as powerful tools in basic and translational research. By definition, they self-organize, but the structures formed are often heterogen ...

American Association for the Advancement of Science2022

Investigating receptor-mediated antibody transcytosis using blood-brain barrier organoid arrays

Nathalie Brandenberg, Camilla Carla Alma Ceroni

Background The pathways that control protein transport across the blood-brain barrier (BBB) remain poorly characterized. Despite great advances in recapitulating the human BBB in vitro, current models are not suitable for systematic analysis of the molecul ...

BMC2021

Robust Phase Unwrapping via Deep Image Prior for Quantitative Phase Imaging

Matthias Lütolf, Michaël Unser, Nathalie Brandenberg, Thanh-An Michel Pham, Fangshu Yang

Quantitative phase imaging (QPI) is an emerging label-free technique that produces images containing morphological and dynamical information without contrast agents. Unfortunately, the phase is wrapped in most imaging system. Phase unwrapping is the comput ...

2021

Diagnostic tools and CFTR functional assays in cystic fibrosis: utility and availability in Switzerland

Nathalie Brandenberg

Cystic fibrosis (CF) is a genetic disease caused by a bi-allelic mutation of the cystic fibrosis transmembrane conductance regulator (CFTR) gene. When the diagnosis cannot be confirmed by a positive sweat test or/and the identification of two CF-causing va ...

E M H SWISS MEDICAL PUBLISHERS LTD2021

Device for manipulating mammalian cells with high collection capability

Matthias Lütolf, Nathalie Brandenberg, Sylke Höhnel

2021

Hydrogel-based milliwell arrays for standardized and scalable retinal organoid cultures

Matthias Lütolf, Nathalie Brandenberg

The development of improved methods to culture retinal organoids is relevant for the investigation of mechanisms of retinal development under pathophysiological conditions, for screening of neuroprotective compounds, and for providing a cellular source for ...

Nature Research2020

Homeostatic mini-intestines through scaffold-guided organoid morphogenesis

Matthias Lütolf, Yoji Tabata, Nathalie Brandenberg, Nikolche Gjorevski, Mikhail Nikolaev, Olga Mitrofanova, Nicolas Antoine Vincent Broguiere, Bilge Sen Elçi, Devanjali Dutta

Epithelial organoids, such as those derived from stem cells of the intestine, have great potential for modelling tissue and disease biology(1-4). However, the approaches that are used at present to derive these organoids in three-dimensional matrices(5,6)r ...

NATURE PUBLISHING GROUP2020

High-throughput automated organoid culture via stem-cell aggregation in microcavity arrays

Gerardo Turcatti, Matthias Lütolf, Nathalie Brandenberg, Fabien Kuttler, Krisztian Homicsko, Nikolche Gjorevski, George Coukos, Camilla Carla Alma Ceroni

Stem-cell-derived epithelial organoids are routinely used for the biological and biomedical modelling of tissues. However, the complexity, lack of standardization and quality control of stem cell culture in solid extracellular matrices hampers the routine ...

NATURE PUBLISHING GROUP2020

Pharmacological induction of a progenitor state for the efficient expansion of primary human hepatocytes

Matthias Lütolf, Didier Trono, Nathalie Brandenberg, Marc Samuel Friedli, Marco Cassano, Carmen Maria Unzu Ezquerro, Evaristo Jose Planet Letschert

The liver is an organ with strong regenerative capacity, yet primary hepatocytes have a low amplification potential in vitro, a major limitation for the cell-based therapy of liver disorders and for ex vivo biological screens. Induced pluripotent stem cell ...

2019

Towards high throughput drug screening for human retina organoids

Roland Moser, Matthias Lütolf, Nathalie Brandenberg

MARY ANN LIEBERT, INC2018

Organoid tissue engineering

Matthias Lütolf, Yoji Tabata, Nathalie Brandenberg, Nikolche Gjorevski, Mikhail Nikolaev

The invention relates to methods for developing and maintaining organoids and the organoids produced thereby. ...

2018

Organoid arrays

Matthias Lütolf, Nathalie Brandenberg

The invention provides methods for producing arrays of organoids, the arrays thereof and uses of such arrays. ...

2018

Advanced technologies to control stem-cell based organogenesis

Nathalie Brandenberg

Organoids represent the first in vitro cell culture systems that closely resemble native tissues in terms of cellular composition, architecture and key aspects of physiology. Through the discovery of these cultures, researchers could demonstrate that stem ...

EPFL2017