Publications by Adrian Ranga | EPFL Graph Search

Arrays of discrete cell culture microenvironments, methods of making such arrays and uses thereof

The invention pertains to a combinatorial method of identifying the hydrogel formulations controlling phenotype and fate of difficult-to-culture cell types. ...

2016

Defined three-dimensional microenvironments boost induction of pluripotency

Matthias Lütolf, Yoji Tabata, Adrian Ranga, Yuya Okawa, Alessandra Piersigilli, Massimiliano Caiazzo

Since the discovery of induced pluripotent stem cells (iPSCs), numerous approaches have been explored to improve the original protocol, which is based on a two-dimensional (2D) cell-culture system. Surprisingly, nothing is known about the effect of a more ...

Nature Publishing Group2016

3D niche microarrays for systems-level analyses of cell fate

Matthias Lütolf, Katarzyna Mosiewicz, Adrian Ranga, Samy Gobaa, Andrea Negro, Yuya Okawa

The behaviour of mammalian cells in a tissue is governed by the three-dimensional (3D) microenvironment and involves a dynamic interplay between biochemical and mechanical signals provided by the extracellular matrix (ECM), cell–cell interactions and solub ...

Nature Publishing Group2014

3D Reconstitution of the Patterned Neural Tube from Embryonic Stem Cells

Matthias Lütolf, Adrian Ranga

Inducing organogenesis in 3D culture is an important aspect of stem cell research. Anterior neural structures have been produced from large embryonic stem cell (ESC) aggregates, but the steps involved in patterning such complex structures have been ill def ...

Cell Press2014

Bioengineering approaches to guide stem cell-based organogenesis

Matthias Lütolf, Adrian Ranga, Nikolche Gjorevski

During organogenesis, various molecular and physical signals are orchestrated in space and time to sculpt multiple cell types into functional tissues and organs. The complex and dynamic nature of the process has hindered studies aimed at delineating morpho ...

Company of Biologists2014

Drug discovery through stem cell-based organoid models

Matthias Lütolf, Adrian Ranga, Nikolche Gjorevski

The development of new drugs is currently a long and costly process in large part due to the failure of promising drug candidates identified in initial in vitro screens to perform as intended in vivo. New approaches to drug screening are being developed wh ...

Elsevier2014

Artificial three-dimensional niches deconstruct pancreas development in vitro

Matthias Lütolf, Anne Grapin-Botton, Chiara Greggio, Adrian Ranga, Samy Gobaa, Filippo De Franceschi

In the context of a cellular therapy for diabetes, methods for pancreatic progenitor expansion and subsequent differentiation into insulin-producing beta cells would be extremely valuable. Here we establish three-dimensional culture conditions in Matrigel ...

Company Of Biologists Ltd2013

Heparin-binding domain of fibrin(ogen) binds growth factors and promotes tissue repair when incorporated within a synthetic matrix

Matthias Lütolf, Mikaël Martino, Jeffrey Alan Hubbell, Priscilla Suhasna Maithili Briquez, Adrian Ranga

By binding growth factors (GFs), the ECM tightly regulates their activity. We recently reported that the heparin-binding domain II of fibronectin acts as a promiscuous high-affinity GF-binding domain. Here we hypothesized that fibrin, the provisional ECM d ...

National Academy of Sciences2013

Systems Biology of 3D Stem Cell-Niche Interactions

Adrian Ranga

EPFL2013

Keynote: Deconstructing and reconstructing stem cell niches

Matthias Lütolf, Adrian Ranga, Samy Gobaa

Wiley-Blackwell2012

High-throughput approaches for the analysis of extrinsic regulators of stem cell fate

Matthias Lütolf, Adrian Ranga

The complexity of stem cell niches poses a tremendous challenge to understanding mechanisms of extrinsic regulation of stem cell fate. In order to better understand niche signaling and its effect on stem cell fate choices, in vitro systems are being engine ...

2012

Elucidating the Role of Matrix Stiffness in 3D Cell Migration and Remodeling

Matthias Lütolf, Katarzyna Mosiewicz, Adrian Ranga, Philipp Sebastian Lienemann, Martin Ehrbar

Reductionist in vitro model systems which mimic specific extracellular matrix functions in a highly controlled manner, termed artificial extracellular matrices (aECM), have increasingly been used to elucidate the role of cell-ECM interactions in regulating ...

Elsevier2011

Engineering 3D cell instructive microenvironments by rational assembly of artificial extracellular matrices and cell patterning

Matthias Lütolf, Adrian Ranga, Martin Ehrbar

Engineered artificial microenvironments hold enormous potential as models to study developmental, physiological, pathological, and regenerative processes under highly defined conditions. Such platforms aim at bridging the gap between traditional in vitro 2 ...

2011