Publications by Nadine Aït Bouziad

An approach to characterize mechanisms of action of anti-amyloidogenic compounds in vitro and in situ

Amyloid aggregation is associated with neurodegenerative disease and its modulation is a focus of drug development. We developed a chemical proteomics pipeline to probe the mechanism of action of anti-amyloidogenic compounds. Our approach identifies putati ...

Springer Science and Business Media LLC2025

Differential role of C-terminal truncations on alpha-synuclein pathology and Lewy body formation

Graham Knott, Mary-Claude Croisier-Coeytaux, Anass Chiki, Nadine Aït Bouziad, Romain Hamelin, Anne-Laure Mahul Mellier, Niran Maharjan, Melek Firat Altay, Jonathan Jean-Pierre Ricci, Somanath Jagannath, Galina Limorenko, Salvatore Novello, Yllza Jasiqi

Alpha-synuclein (aSyn) post-translational modifications (PTM), especially phosphorylation at serine 129 and C-terminal truncations, are highly enriched in Lewy bodies (LB), Lewy neurites, and other pathological aggregates in Parkinson's disease and synucle ...

Springer Science and Business Media LLC2025

Unraveling the complexity of amyloid polymorphism using gold nanoparticles and cryo-EM

Hilal Lashuel, Anass Chiki, Nadine Aït Bouziad, Francesco Stellacci, Paulo Henrique Jacob Silva, Marie Mueller, Zekiye Pelin Güven, Sophie Vieweg, Urszula Beata Cendrowska

Increasing evidence suggests that amyloid polymorphism gives rise to different strains of amyloids with distinct toxicities and pathology-spreading properties. Validating this hypothesis is challenging due to a lack of tools and methods that allow for the ...

National Academy of Sciences2020

Phosphorylation of the overlooked tyrosine 310 regulates the structure, aggregation, and microtubule- and lipid-binding properties of Tau

Hilal Lashuel, Anass Chiki, Nadine Aït Bouziad, Galina Limorenko

The microtubule-associated protein Tau is implicated in the pathogenesis of several neurodegenerative disorders, including Alzheimer's disease. Increasing evidence suggests that post-translational modifications play critical roles in regulating Tau's norma ...

2020

A method for preparing phfs-like tau aggregates

Hilal Lashuel, Nadine Aït Bouziad

The invention relates to a method for preparing PHFs-like Tau aggregates and to a method for identifying compounds that are inhibitors of Tau protein aggregation, blockers of Tau seeding and propagation, and imaging agents that specifically bind PHF. ...

2019

Resolving molecule-specific information in dynamic lipid membrane processes with multi-resonant infrared metasurfaces

Hilal Lashuel, Nadine Aït Bouziad, Hatice Altug, Odeta Limaj, Daniel Rodrigo Lopez, Christopher David Kelly, Andreas Tittl, Aurélian Michel John-Herpin

A multitude of biological processes are enabled by complex interactions between lipid membranes and proteins. To understand such dynamic processes, it is crucial to differentiate the constituent biomolecular species and track their individual time evolutio ...

Nature Research2018

Discovery and characterization of novel stable tau oligomeric complexes: Implications for the role of Tau/phospholipid interactions in regulating its functions in health and disease

Hilal Lashuel, Nadine Aït Bouziad, Gizem Zorludemir, Anne-Laure Mahul Mellier

The microtubule-associated protein Tau plays a central role in the pathogenesis of Alzheimer's disease. Although Tau interaction with membranes is thought to affect some of its physiological functions and its aggregation properties, the sequence determinan ...

Nature Publishing Group2017

Sequence and structural determinants of Tau aggregation and seeding capacity: Implications for Neurofibrillary Tangle formation and Tau toxicity in Alzheimer's disease

Nadine Aït Bouziad

Despite decades of research in Alzheimerâ s disease (AD), no treatment has been found to efficiently prevent or cure its progression. The microtubule binding protein Tau plays central roles in the pathogenesis of AD and is found, in the form of paired hel ...

EPFL2017

Microtubule-Binding R3 Fragment from Tau Self-Assembles into Giant Multistranded Amyloid Ribbons

Raffaele Mezzenga, Hilal Lashuel, Nadine Aït Bouziad

Tau protein and its fragments self-assemble into amyloid fibrils in the presence of polyanions, such as heparin. By combining microscopy, scattering, and spectroscopy techniques, we studied the aggregation of the 26-mer Tau-derived peptide alone, Tau306-32 ...

Wiley-VCH Verlag GmbH2016

Elucidating the Role of Site-Specific Nitration of alpha-Synuclein in the Pathogenesis of Parkinson's Disease via Protein Semisynthesis and Mutagenesis

Hilal Lashuel, Anass Chiki, Nadine Aït Bouziad, Ritwik Burai

Parkinsons disease (PD) is characterized by the loss of dopaminergic neurons in the substantia nigra and the presence of intraneuronal inclusions consisting of aggregated and post-translationally modified a-synuclein (a-syn). Despite advances in the chemic ...

Amer Chemical Soc2015

The novel Parkinson's disease linked mutation G51D attenuates in vitro aggregation and membrane binding of alpha-synuclein, and enhances its secretion and nuclear localization in cells

Hilal Lashuel, Ana Jovicic, Martial Mbefo Kamdem, Nadine Aït Bouziad, Mohamed Bilal Fares

A novel mutation in the alpha-Synuclein (alpha-Syn) gene "G51D" was recently identified in two familial cases exhibiting features of Parkinson's disease (PD) and multiple system atrophy (MSA). In this study, we explored the impact of this novel mutation on ...

Oxford University Press2014