Publications by Julien Michel Ghaye

Image processing on reconfigurable hardware for continuous monitoring of fluorescent biomarkers in cell cultures

Fluorescence microscopy is a widespread tool in biological research. It is the primary modality for bioimaging and empowers the study and analysis of multitudes of biological processes. It can be applied to fixed biosamples, that is samples with frozen bio ...

EPFL2015

Quantitative Estimation of Biological Cell Surface Receptors by Segmenting Conventional Fluorescence Microscopy Images

Paolo Silacci, Guy Vergères, Giovanni De Micheli, Julien Michel Ghaye, Sandro Carrara, Chiara Succa, Danilo Demarchi

State-of-the-art techniques for measuring and monitoring gene level expression rely on messenger RNA (mRNA) extraction and quantification, usually based on the concept of reverse transcription polymerase chain reaction. In this paper, we take adva ...

Ieee2014

Empowering Low-Cost CMOS Cameras by Image Processing to Reach Comparable Results with Costly CCDs

Yusuf Leblebici, Giovanni De Micheli, Gözen Köklü, Julien Michel Ghaye, Sandro Carrara

Despite the huge research effort to improve the performance of the complementary metal oxide semiconductor (CMOS) image sensors, charge-coupled devices (CCDs) still dominate the cell biology-related conventional fluorescence microscopic imaging market wher ...

2013

Image thresholding techniques for localization of sub-resolution fluorescent biomarkers

Paolo Silacci, Guy Vergères, Giovanni De Micheli, Julien Michel Ghaye, Sandro Carrara, Madhura Avinash Kamat

In this article, we explore adaptive global and local segmentation techniques for a lab-on-chip nutrition monitoring system (NutriChip). The experimental setup consists of Caco-2 intestinal cells that can be artificially stimulated to trigger an immune res ...

Wiley-Blackwell2013

NutriChip: Nutrition Analysis meets Microfluidics

Martinus Gijs, Paolo Silacci, Guy Vergères, Gözen Köklü, Julien Michel Ghaye, Sandro Carrara, Hamideh Jafarpoorchekab, Christine Ruffert

This focus article introduces the concept of NutriChip, an integrated microfluidic platform for investigating the potential of the immuno-modulatory function of dairy food. The core component of the NutriChip is a miniaturized artificial human gastrointest ...

Royal Society of Chemistry2013

Quantification of Sub-resolution Sized Targets in Cell Fluorescent Imaging

Giovanni De Micheli, Julien Michel Ghaye, Sandro Carrara

We introduce two methods for quantifying and evaluating the amount of surface receptors within a group of cells from fluorescence microscope images. First, the average fluorescence intensity method (AFIM), based on the fluores- cent pixels average intensit ...

2012

Quantitative Comparison of Commercial CCD and Custom-Designed CMOS Camera for Biological Applications

René Beuchat, Yusuf Leblebici, Giovanni De Micheli, Gözen Köklü, Julien Michel Ghaye, Sandro Carrara

In biological applications and systems where even the smallest details have a meaning, CCD cameras are mostly preferred and they hold most of the market share despite their high costs. In this paper, we propose a custom-designed CMOS camera to compete with ...

2012

Simulated biological cells for receptor counting in fluorescence imaging

Giovanni De Micheli, Julien Michel Ghaye, Sandro Carrara

Digital image processing and epi-fluorescence microscopy provide one of the main and basic tools for living biological cells analysis and studying. Developing, testing and comparing those image processing methods properly require the use of a controlled en ...

2012