Publications by Livan Bladimir Alonso Sarduy

Molecular Mechanism of Flocculation Self-Recognition in Yeast and Its Role in Mating and Survival

Jens Nielsen, Sandor Kasas, Livan Bladimir Alonso Sarduy

We studied the flocculation mechanism at the molecular level by determining the atomic structures of N-Flo1p and N-Lg-Flo1p in complex with their ligands. We show that they have similar ligand binding mechanisms but distinct carbohydrate specificities and ...

American Society for Microbiology2015

Real-Time Monitoring of Protein Conformational Changes Using a Nano-Mechanical Sensor

Giovanni Dietler, Paolo De Los Rios, Dusan Vobornik, Sandor Kasas, Livan Bladimir Alonso Sarduy, Fabrizio Benedetti, Giovanni Longo

Proteins can switch between different conformations in response to stimuli, such as pH or temperature variations, or to the binding of ligands. Such plasticity and its kinetics can have a crucial functional role, and their characterization has taken center ...

Public Library of Science2014

A universal fluid cell for the imaging of biological specimens in the atomic force microscope

Charles Roduit, Giovanni Dietler, Sandor Kasas, Livan Bladimir Alonso Sarduy, Giovanni Longo, Bhaskar Saha

Recently, atomic force microscope (AFM) manufacturers have begun producing instruments specifically designed to image biological specimens. In most instances, they are integrated with an inverted optical microscope, which permits concurrent optical and AFM ...

Wiley-Blackwell2013

Rapid detection of bacterial resistance to antibiotics using AFM cantilevers as nanomechanical sensors

Giovanni Dietler, Sandor Kasas, Justin Terence Notz Pekkanen, Livan Bladimir Alonso Sarduy, Giovanni Longo, Laura Rio Marques

The widespread misuse of drugs has increased the number of multiresistant bacteria1, and this means that tools that can rapidly detect and characterize bacterial response to antibiotics are much needed in the management of infections. Various techniques, s ...

Nature Publishing Group2013

Nanoscale motion detector

Giovanni Dietler, Sandor Kasas, Livan Bladimir Alonso Sarduy, Giovanni Longo

Motion detector comprising a flexible support (1,5) adapted to hold at least one object (6-9), a sensor (4) for measuring the displacement of said support (1) and processing means for differentiating the fluctuations of said support (1) from those induced ...

2013

Time-Lapse AFM Imaging of DNA Conformational Changes Induced by Daunorubicin

Giovanni Dietler, Sandor Kasas, Livan Bladimir Alonso Sarduy, Giovanni Longo

Cancer is a major health issue that absorbs the attention of a large part of the biomedical research. Intercalating agents bind to DNA molecules and can inhibit their synthesis and transcription; thus, they are increasingly used as drugs to fight cancer. I ...

American Chemical Society2013

Study of Topoisomerase-DNA Interaction Using Atomic Force Microscopy

Livan Bladimir Alonso Sarduy

The aim of this thesis was to explore the topoisomerase-DNA interactions using atomic force microscopy (AFM) in a liquid environment. Firstly, we present a programmable microcontroller-driven injection system for the exchange of an imaging medium while usi ...

EPFL2012

Differential peptide binding to CD40 evokes counteractive responses

Charles Roduit, Giovanni Dietler, Sandor Kasas, Livan Bladimir Alonso Sarduy, Bhaskar Saha

The antigen-presenting cell-expressed CD40 is implied in the regulation of counteractive immune responses such as induction of pro-inflammatory and anti-inflammatory cytokines interleukin (IL)-12 and IL-10, respectively. The mechanism of this duality in CD ...

Elsevier2012

Differential peptide binding to CD40 evokes counteractive responses (vol 73, pg 465, 2012)

Charles Roduit, Giovanni Dietler, Sandor Kasas, Livan Bladimir Alonso Sarduy, Bhaskar Saha

Elsevier2012

FM-AFM constant height imaging and force curves: high resolution study of DNA-tip interactions

Giovanni Dietler, Dusan Vobornik, Susana del Carmen Tobenas Borron, Livan Bladimir Alonso Sarduy, Andrea Cerreta

Interaction of the atomic force microscopy (AFM) tip with the sample can be invasive for soft samples. Frequency Modulation (FM) AFM is gentler because it allows scanning in the non-contact regime where only attractive forces exist between the tip and the ...

Wiley-Blackwell2012

Human topoisomerase II-DNA interaction study by using atomic force microscopy

Charles Roduit, Giovanni Dietler, Sandor Kasas, Livan Bladimir Alonso Sarduy

AFM; DNA topology; Human topoisomerase II; Binding geometry; Time-lapse imaging; Protein–DNA interaction ...

Elsevier2011

Microcontroller-driven fluid-injection system for atomic force microscopy

Giovanni Dietler, Sandor Kasas, Livan Bladimir Alonso Sarduy

We present a programmable microcontroller-driven injection system for the exchange of imaging medium during atomic force microscopy. Using this low-noise system, high-resolution imaging can be performed during this process of injection without disturbance. ...

American Institute of Physics2010