Publications by Ata Tuna Ciftlik

Computer-Based Intensity Measurement Assists Pathologists in Scoring Phosphatase and Tensin Homolog Immunohistochemistry - Clinical Associations in NSCLC Patients of the European Thoracic Oncology Platform Lungscape Cohort

Thomas Geiger, Ruben Casanova, Ata Tuna Ciftlik

Introduction: Phosphatase and tensin homolog (PTEN) loss is frequently observed in NSCLC and associated with both phosphoinositide 3-kinase activation and tumoral immunosuppression. PTEN immunohistochemistry is a valuable readout, but lacks standardized st ...

2018

Microfluidic network device

Martinus Gijs, Pierre Joris, Ata Tuna Ciftlik, Diego Gabriel Dupouy

Microfluidic network device (2) configured to supply reagents to a biological tissue sampling device (1), comprising a plurality of microfluidic inlet channels (12) connected to respective sources of said reagents, at least one common outlet channel (22), ...

2017

Method for quantitative measurement of a biomarker by in situ immunofluorescence and uses thereof

Martinus Gijs, Déborah Heintze, Ata Tuna Ciftlik, Diego Gabriel Dupouy

The invention relates to a method for quantitative measurement of a biomarker by in situ immunofluorescence and uses thereof. In particular, the invention relates to a method which is a useful tool for use in the field of diagnosis, prevention and/or treat ...

2017

Continuous quantification of HER2 expression by microfluidic precision immunofluorescence estimates HER2 gene amplification in breast cancer

Martinus Gijs, Déborah Heintze, Ata Tuna Ciftlik, Diego Gabriel Dupouy, Maryse Fiche

Chromogenic immunohistochemistry (IHC) is omnipresent in cancer diagnosis, but has also been criticized for its technical limit in quantifying the level of protein expression on tissue sections, thus potentially masking clinically relevant data. Shifting f ...

Nature Research2016

Programming and use of Parylene C fluorescence as a quantitative on-chip reference

Martinus Gijs, Ata Tuna Ciftlik, Diego Gabriel Dupouy, Joan Teixidor

A large number of lab-on-a-chip applications use fluorescence for quantifying biological entities. In such applications, incorporation of a stable on-chip fluorescent reference source would be highly desirable in order to compensate for instrumental parame ...

Royal Soc Chemistry2014

Fabrication of Biocompatible Monolithic Microchannels with High Pressure-Resistance Using Direct Polymerization of PEG-Modified PMMA

Martinus Gijs, Ata Tuna Ciftlik, Elena Bianchi

Withstanding high pressures in polymeric microchannels is an important requirement for many biological applications. Here, a simple direct polymerization through a polyester photomask is applied to obtain monolithic polyethylene glycol (PEG)-modified poly( ...

Wiley-Blackwell2014

Sample processing device with detachable slide

Martinus Gijs, Ata Tuna Ciftlik

A biological and chemical sample processing device that b. comprises a high pressure-resistant, shallow and wide area microfluidic chamber having at least one wall formed by a detachable slide containing samples such as immobilized entities, biological sam ...

2013

Characterization of short incubation time effects on chromogen signal obtained by HER2-expressing breast carcinomas using microfluidic immunohistochemistry

Martinus Gijs, Ata Tuna Ciftlik, Hans-Anton Lehr

2013

Opportunities for high-pressure microfluidics in biomedical applications

Ata Tuna Ciftlik

Pressure lies at the basis of operation of most microfluidic systems, and is a determinant factor in the extent of miniaturization and in limiting the throughput and time constant of the microfluidic assays. Despite the apparent importance given to fluidic ...

EPFL2013

Microfluidic processor allows rapid HER2 immunohistochemistry of breast carcinomas and significantly reduces ambiguous (2+) read-outs

Martinus Gijs, Ata Tuna Ciftlik, Hans-Anton Lehr

Biomarker analysis is playing an essential role in cancer diagnosis, prognosis, and prediction. Quantitative assessment of immunohistochemical biomarker expression on tumor tissues is of clinical relevance when deciding targeted treatments for cancer patie ...

National Academy of Sciences2013

Programmable parylene-C bonding layer fluorescence for storing information on microfluidic chips

Martinus Gijs, Ata Tuna Ciftlik, Diego Gabriel Dupouy

We demonstrate data storage on glass/silicon microfluidic devices fabricated using parylene-C as a bonding layer. In particular, we report intermediate parylene-C bonding layer fluorescence (iPBLF) and its use as an on-chip medium for data storage by dynam ...

Royal Society of Chemistry2013

Demonstration of inertial focusing in straight microfluidic channels with high Reynolds numbers up to turbulence onset

Martinus Gijs, Ata Tuna Ciftlik

2013

Use of a Parylene-C bonding layer fluorescence as reference for on-chip imaging and detection applications

Martinus Gijs, Ata Tuna Ciftlik, Diego Gabriel Dupouy

2013

High Throughput-Per-Footprint Inertial Focusing

Martinus Gijs, Maxime Ettori, Ata Tuna Ciftlik

Matching the scale of microfluidic flow systems with that of microelectronic chips for realizing monolithically integrated systems still needs to be accomplished. However, this is appealing only if such re-scaling does not compromise the fluidic throughput ...

Wiley-V C H Verlag Gmbh2013

Parylene to silicon nitride bonding for post-integration of high pressure microfluidics to CMOS devices

Martinus Gijs, Ata Tuna Ciftlik

High pressure-rated channels allow microfluidic assays to be performed on a smaller footprint while keeping the throughput, thanks to the higher enabled flow rates, opening perspectives for cost-effective integration of CMOS chips to microfluidics circuits ...

Royal Society of Chemistry2012

Dynamically programmable parylene-C bonding layer fluorescence for re-writable data storage on a microfluidic chip

Martinus Gijs, Ata Tuna Ciftlik

Wafer bonding using parylene-C to Si, SiO2 and SiN surfaces has recently been shown to enable high pressure-resistant microfluidic devices with metallization and CMOS-integration capability. Here, we report characterization of intermediate parylene-C bondi ...

2012

Parylene to silicon-nitride bonding for easy post-integration of high-pressure microfluidics to CMOS devices

Martinus Gijs, Maxime Ettori, Ata Tuna Ciftlik

2011

A low-temperature parylene-to-silicon dioxide bonding technique for high-pressure microfluidics

Martinus Gijs, Ata Tuna Ciftlik

We introduce a new low-temperature (280 °C) parylene-to-SiO2 bonding process with high device yield (>90%) for the fabrication and integration of high-pressure-rated microfluidic chips. Pull tests demonstrate a parylene-to-SiO2 bonding strength of 10 ± 3 M ...

Institute of Physics2011

Low temperature pyrex/silicon wafer bonding via a single intermediate parylene layer

Martinus Gijs, Ata Tuna Ciftlik

We introduce a new low temperature (280 °C) parylene-C wafer bonding technique, where parylene-C bonds directly a Pyrex wafer to a silicon wafer with either a Si, SiO2 or Si3N4 surface with a bonding strength up to 23 MPa. The technique uses a single layer ...

IEEE2011

Chaotic mixing using source-sink microfluidic flows in a PDMS chip

Abdeljalil Sayah, Martinus Gijs, Caroline Vandevyver, Venkataragavalu Sivagnanam, Hüseyin Cumhur Tekin, Ata Tuna Ciftlik

We present an active fixed-volume mixer based on the creation of multiple source–sink microfluidic flows in a polydimethylsiloxane (PDMS) chip without the need of external or internal pumps. To do so, four different pressure-controlled actuation chambers a ...

Springer-Verlag2011