Publications by Arne Seitz | EPFL Graph Search

Development of image analysis tool to evaluate Langerhans cell migration after exposure to isothiazolinones

Incidence of allergic contact dermatitis (ACD) is rising worldwide as a consequence of raising chemical exposure, especially from biocides present in common water-based products such as cosmetics and household products. Understanding the biological process ...

SPRINGER HEIDELBERG2025

ABBA+BraiAn, an integrated suite for whole-brain mapping, reveals brain-wide differences in immediate-early genes induction upon learning

Arne Seitz, Johannes Gräff, Nicolas René Chiaruttini

Elsevier BV2025

MarrowQuant 2.0: A Digital Pathology Workflow Assisting Bone Marrow Evaluation in Experimental and Clinical Hematology

Olivier Burri, Giovanna Ambrosini, Bart Deplancke, Arne Seitz, Olaia Maria Naveiras Torres-Quiroga, Frédérica Schyrr, Rita Sarkis, Mariangela Costanza, Martin Weigert

2023

Community-developed checklists for publishing images and image analyses

Arne Seitz

Images document scientific discoveries and are prevalent in modern biomedical research. Microscopy imaging in particular is currently undergoing rapid technological advancements. However, for scientists wishing to publish obtained images and image-analysis ...

NATURE PORTFOLIO2023

An Open-Source Whole Slide Image Registration Workflow at Cellular Precision Using Fiji, QuPath and Elastix

Olivier Burri, Jessica Sordet, Arne Seitz, Romain Guiet, Nicolas René Chiaruttini

Image analysis workflows for Histology increasingly require the correlation and combination of measurements across several whole slide images. Indeed, for multiplexing, as well as multimodal imaging, it is indispensable that the same sample is imaged multi ...

FRONTIERS MEDIA SA2022

QUAREP-LiMi: a community endeavor to advance quality assessment and reproducibility in light microscopy

Arne Seitz

The community-driven initiative Quality Assessment and Reproducibility for Instruments & Images in Light Microscopy (QUAREP-LiMi) wants to improve reproducibility for light microscopy image data through quality control (QC) management of instruments and im ...

2021

Biotin-NeutrAvidin Mediated Immobilization of Polymer Micro- and Nanoparticles on T Lymphocytes

Harm-Anton Klok, Olivier Burri, Maxime Ayer, Arne Seitz, Romain Guiet

Cells are powerful carriers that can help to improve the delivery of nanomedicines. One approach to use cells as carriers is to immobilize the nanoparticulate cargo on the cell surface. While a plethora of chemical conjugation strategies are available to b ...

2021

QUAREP-LiMi: A community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy

Andrea Bassi, Antje Keppler, Arne Seitz

A modern day light microscope has evolved from a tool devoted to making primarily empirical observations to what is now a sophisticated , quantitative device that is an integral part of both physical and life science research. Nowadays, microscopes are fou ...

WILEY2021

T Cell-Mediated Transport of Polymer Nanoparticles across the Blood-Brain Barrier

Harm-Anton Klok, Olivier Burri, Maxime Ayer, Arne Seitz, Romain Guiet, Markus Schuster

Delivery of therapeutics to the central nervous system (CNS) is challenging due to the presence of the blood-brain barrier (BBB). Amongst various approaches that have been explored to facilitate drug delivery to the CNS, the use of cells that have the intr ...

WILEY2020

W2S: Microscopy Data with Joint Denoising and Super-Resolution for Widefield to SIM Mapping

Daniel Sage, Sabine Süsstrunk, Thierry Laroche, Arne Seitz, Ruofan Zhou, Majed El Helou

In fluorescence microscopy live-cell imaging, there is a critical trade-off between the signal-to-noise ratio and spatial resolution on one side, and the integrity of the biological sample on the other side. To obtain clean high-resolution (HR) images, one ...

2020

Hookworms Evade Host Immunity by Secreting a Deoxyribonuclease to Degrade Neutrophil Extracellular Traps

Manuel Kulagin, Arne Seitz, Nicola Harris, Romain Guiet, Beatrice Volpe, Kathleen Shah, Tiffany Babette Angélique Bouchery, Mati André Marie Moyat

Hookworms cause a major neglected tropical disease, occurring after larvae penetrate the host skin. Neutrophils are phagocytes that kill large pathogens by releasing neutrophil extracellular traps (NETs), but whether they target hookworms during skin infec ...

CELL PRESS2020

Development of Sample-Adaptable Holders for Lightsheet Microscopy

Olivier Burri, Thierry Laroche, Arne Seitz, Lalit Kumar Dubey

Multi-user core microscopy facilities are often faced with the challenge to adapt or modify existing instruments. This is essential in order to fulfill the requirements of the user community, who wants to image a wide range of model organisms with varying ...

2019

Cellular Uptake and Intracellular Trafficking of Poly(N-(2-Hydroxypropyl) Methacrylamide)

Harm-Anton Klok, Olivier Burri, Graham Knott, Arne Seitz, Claudia Battistella, Anders Meibom, Romain Guiet, Stéphane Laurent Escrig

Cellular uptake and intracellular trafficking of polymer conjugates or polymer nanoparticles is typically monitored using fluorescence-based techniques such as confocal microscopy. While these methods have provided a wealth of insight into the internalizat ...

American Chemical Society2019

TRACMIT: An effective pipeline for tracking and analyzing cells on micropatterns through mitosis

Olivier Burri, Pierre Gönczy, Arne Seitz, Benita Wolf

The use of micropatterns has transformed investigations of dynamic biological processes by enabling the reproducible analysis of live cells using time-lapse fluorescence microscopy. With micropatterns, thousands of individual cells can be efficiently image ...

2017

A formin-nucleated actin aster concentrates cell wall hydrolases for cell fusion in fission yeast

Thierry Laroche, Arne Seitz, Raphaël Groux, Omaya Pierre Dudin

Cell-cell fusion is essential for fertilization. For fusion of walled cells, the cell wall must be degraded at a precise location but maintained in surrounding regions to protect against lysis. In fission yeast cells, the formin Fus1, which nucleates linea ...

Rockefeller University Press2015

Antibody-Mediated Trapping of Helminth Larvae Requires CD11b and Fc gamma Receptor I

Manuel Kulagin, Arne Seitz, Nicola Harris, Benjamin John Marsland, Julia Esser-von Bieren, Romain Guiet, Beatrice Volpe, Duncan-Bruce Sutherland

Infections with intestinal helminths severely impact on human and veterinary health, particularly through the damage that these large parasites inflict when migrating through host tissues. Host immunity often targets the motility of tissue-migrating helmin ...

American Association of Immunologists ; Oxford University Press2015

Correlative multicolor 3D SIM and STORM microscopy

Mathias Fournier, Pierre Gönczy, Virginie Hamel, Arne Seitz, Isabelle Fluckiger, Paul Guichard, Romain Guiet

Within the last decade, super-resolution methods that surpass the diffraction limit of light microscopy have provided invaluable insight into a variety of biological questions. Each of these approaches has inherent advantages and limitations, such that the ...

Optical Soc Amer2014

Antibodies Trap Tissue Migrating Helminth Larvae and Prevent Tissue Damage by Driving IL-4Rα-Independent Alternative Differentiation of Macrophages

Fei Chen, Arne Seitz, Ilaria Mosconi, Nicola Harris, Julia Esser-von Bieren, Romain Guiet, Beatrice Volpe, Alessandra Piersigilli

Approximately one-third of the world's population suffers from chronic helminth infections with no effective vaccines currently available. Antibodies and alternatively activated macrophages (AAM) form crucial components of protective immunity against chall ...

Public Library of Science2013

Automated analysis of single stem cells in microfluidic traps

Matthias Lütolf, Olivier Burri, Alessandra Griffa, Stefan Kobel, Arne Seitz, Mukul Girotra

We report a reliable strategy to perform automated image cytometry of single (non-adherent) stem cells captured in microfluidic traps. The method rapidly segments images of an entire microfluidic chip based on the detection of horizontal edges of microflui ...

2012