Publications by Carlotta Guiducci

Electrokinetic microdevices for biological sample processing

Carlotta Guiducci, Pierre-Emmanuel Marie Thiriet, Gloria Porro

Microsystems combining fluid dynamics and electric-field-induced forces have emerged as powerful tools for manipulating and isolating biological species. Advances in electrokinetic theory, combined with optimized microfabrication processes, are at the core ...

Springer Science and Business Media LLC2024

MarrowCellDLD: a microfluidic method for label-free retrieval of fragile bone marrow-derived cells

Carlotta Guiducci, Gloria Porro

Functional bone marrow studies have focused primarily on hematopoietic progenitors, leaving limited knowledge about other fragile populations, such as bone marrow adipocytes (BMAds) and megakaryocytes. The isolation of these cells is challenging due to rup ...

Nature Portfolio2023

Prediction of Dispersion Rate of Airborne Nanoparticles in a Gas-Liquid Dual-Microchannel Separated by a Porous Membrane: A Numerical Study

Carlotta Guiducci, Zohreh Sheidaei

Recently, there has been increasing attention toward inhaled nanoparticles (NPs) to develop inhalation therapies for diseases associated with the pulmonary system and investigate the toxic effects of hazardous environmental particles on human lung health. ...

MDPI2022

Particle-sorting fluidic device and methods for using thereof

Carlotta Guiducci, Kevin Keim, Gloria Porro

Device, system and method for sorting particles comprised into a fluid are disclosed that improve the current settings of deterministic lateral displacement (DLD)-based microfluidic devices, so to sort and detect small particles such as extracellular vesic ...

2022

Device to carry out semiconductor-based diagnostics

Carlotta Guiducci, Saurabh Tomar

The present invention relates to a device to carry out semiconductor based molecular diagnostics comprising a microfluidic chip carrier 1, an elastomer microfluidic module 2 and a CMOS chip 3, one surface of said microfluidic module facing and contacting o ...

2022

Integration of Ultra-Low Volume Pneumatic Microfluidics with a Three-Dimensional Electrode Network for On-Chip Biochemical Sensing

Carlotta Guiducci, Thomas Ernst, Saurabh Tomar

This paper reports a novel miniaturized pseudo reference electrode (RE) design for biasing Ion Sensitive Field Effect Transistors (ISFETs). It eliminates the need for post-CMOS processing and can scale up in numbers with the CMOS scaling. The presented des ...

2021

Dielectrophoresis detection device

Carlotta Guiducci, Pierre-Emmanuel Marie Thiriet

A dielectrophoretic detection device comprising a chip, with a flow channel having at least one inlet and one outlet, and at least a detection area configured to detect analytes trapped on functionalised beads flowing within said flow channel, a first and ...

2021

Device and method for pre-term birth risk assessment

Carlotta Guiducci, Erick Antonio Garcia Cordero, David Baud, Gloria Porro

The invention relates to a vaginal fluid monitoring device embedded into a feminine sanitary pad, said device comprising a stack of the following elements: An absorbent layer (100) configured to be in proximity to, and collect, a vaginal fluid; and A biose ...

2021

Selective Retrieval of Individual Cells from Microfluidic Arrays Combining Dielectrophoretic Force and Directed Hydrodynamic Flow

Bart Deplancke, Carlotta Guiducci, Kevin Keim, Pierre-Emmanuel Marie Thiriet, Gabriele Gambardella, Jörn Pezoldt

Hydrodynamic-based microfluidic platforms enable single-cell arraying and analysis over time. Despite the advantages of established microfluidic systems, long-term analysis and proliferation of cells selected in such devices require off-chip recovery of ce ...

MDPI2020

Rapid Multianalyte Microfluidic Homogeneous Immunoassay on Electrokinetically Driven Beads

Carlotta Guiducci, Pierre-Emmanuel Marie Thiriet, Danashi Imani Medagoda, Gloria Porro

The simplicity of homogeneous immunoassays makes them suitable for diagnostics of acute conditions. Indeed, the absence of washing steps reduces the binding reaction duration and favors a rapid and compact device, a critical asset for patients experiencing ...

2020

Active Posts in Deterministic Lateral Displacement Devices

Carlotta Guiducci, Kevin Keim

Using electrically connected metal-coated posts in a deterministic lateral displacement (DLD) device and applying electric fields, electrokinetics is used to tune separations, significantly decrease the critical size for separation, and increase the dynami ...

Wiley2019

On‐chip technology for single‐cell arraying, electrorotation‐based analysis and selective release

Samuel Kilchenmann, Carlotta Guiducci, Kevin Keim, Paul Karoly Otto Ery, Mohamed Zakarya Alsaid Ali Rashed

This paper reports a method for label-free single-cell biophysical analysis of multiple cells trapped in suspension by electrokinetic forces. Tri-dimensional pillar electrodes arranged along the width of a microfluidic chamber define actuators for single c ...

2019

Isothermal multiple displacement amplification of DNA templates in minimally buffered conditions using phi29 polymerase

Carlotta Guiducci, Enrico Tenaglia

The isothermal amplification of DNA in minimally buffered conditions allows to perform and monitor nucleic acid amplification with minimal technological and operative requirements. We show in this work how phi29 can operate multiple displacement amplificat ...

Royal Society of Chemistry2018

Comparison against current standards of a DNA aptamer for the label-free quantification of tobramycin in human sera employed for therapeutic drug monitoring

Thierry Buclin, Carlotta Guiducci, Anna Ferretti, Enrico Tenaglia

2018

Label-free identification of activated T lymphocytes through tridimensional microsensors on chip

Carlotta Guiducci, Elena Bianchi, Enrica Rollo, George Coukos, Enrico Tenaglia, Alexandre Harari

Label-free approaches to assess cell properties ideally suit the requirements of cell-based therapeutics, since they permit to characterize cells with minimal perturbation and manipulation, at the benefit of sample recovery and re-employment for treatment. ...

Elsevier2017

Metal-Coated SU-8 Structures for High-Density 3-D Microelectrode Arrays

Samuel Kilchenmann, Carlotta Guiducci, Pietro Maoddi, Enrica Rollo

Electric fields can be effectively used to sense, manipulate, and move particles in lab-on-a-chip devices. Nevertheless, the throughput of such devices is a critical issue, which can be effectively improved by increasing the height of the microchannels. Fo ...

Institute of Electrical and Electronics Engineers2016

Multi-Wire Tri-Gate Silicon Nanowires Reaching Milli-pH Unit Resolution in One Micron Square Footprint

Luca Selmi, Enrico Accastelli, Carlotta Guiducci, Thomas Ernst, Paolo Scarbolo, Pierpaolo Palestri

The signal-to-noise ratio of planar ISFET pH sensors deteriorates when reducing the area occupied by the device, thus hampering the scalability of on-chip analytical systems which detect the DNA polymerase through pH measurements. Top-down nano-sized tri-g ...

Mdpi Ag2016

Analysis of Dielectric Microbead Detection by Impedance Spectroscopy with Nanoribbons

Luca Selmi, Enrico Accastelli, Carlotta Guiducci, Thomas Ernst, Paolo Scarbolo

We present a quantitative numerical analysis of dielectric microbead detection experiments with nanoribbons operated in the AC small signal regime. To this purpose, a comprehensive model of nanoribbon operation in electrolyte environment and with DC/AC sig ...

Ieee2016

Label-Free Detection of Tobramycin in Serum by Transmission-Localized Surface Plasmon Resonance

Laurent Décosterd, Thierry Buclin, Carlotta Guiducci, Anna Ferretti, Giulia Cappi, Fabio Mario Spiga, Michael Beyeler

In order to improve the efficacy and safety of treatments, drug dosage needs to be adjusted to the actual needs of each patient in a truly personalized medicine approach. Key for widespread dosage adjustment is the availability of point-of-care devices abl ...

Amer Chemical Soc2015

More DNA–Aptamers for Small Drugs: A Capture–SELEX Coupled with Surface Plasmon Resonance and High-Throughput Sequencing

Carlotta Guiducci, Fabio Mario Spiga

To address limitations in the production of DNA aptamers against small molecules, we introduce a DNA-based capture-SELEX (systematic evolution of ligands by exponential enrichment) protocol with long and continuous randomized library for more flexibility, ...

ACS Publications2015